火箭推进剂单推-Ⅲ对大鼠肝、肾的毒性效应Studies on the Hepatotoxicity and Nephrotoxicity in Rats Induced by Rocket Propellant DT-Ⅲ
侯立安;张伟;杨丹凤;林本成;马力;袭著革;
摘要(Abstract)：
为探讨新型火箭推进剂单推-Ⅲ(主成分为肼)对大鼠肝、肾的毒性效应及其作用机制,将24只雄性Wistar大鼠随机分为4组:生理盐水对照组,低剂量组、中剂量组和高剂量组,采用灌胃方式染毒,单次染毒剂量分别为0、0.70、2.35、7.05mg·kg-1(以单推-Ⅲ中的肼计算),每天1次,连续染毒7d,眼眶静脉丛取血后腹主动脉放血处死大鼠,称肝、肾重量计算肝、肾的脏器系数,测定大鼠血清中反映肝、肾功能的生化指标,并对肝、肾进行病理学观察.结果表明,1)单推-Ⅲ染毒可导致大鼠出现后肢运动障碍,并显著提高大鼠肝、肾脏器系数及血糖水平;2)病理学观察发现,单推-Ⅲ染毒可导致大鼠肝脏灶性及汇管区少量炎症细胞浸润,肝细胞脂肪变性,肾脏则无明显改变;3)单推-Ⅲ染毒可导致大鼠肝功能异常,部分肝功能指标如谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、直接胆红素(D-BIL)等出现显著升高或降低;4)单推-Ⅲ染毒可导致大鼠肾功能异常,部分肾功能指标如尿素氮(BUN)、尿酸(UA)、肌酐(CREA)等出现显著升高或降低.综合以上实验结果可以看出火箭推进剂单推-Ⅲ对大鼠肝、肾组织可产生一定的毒性效应,并可造成一定程度的中枢神经损伤。
关键词(KeyWords)： 单推-Ⅲ;肼;肝毒性;肾毒性;脏器系数;大鼠
基金项目(Foundation): 军事医学科学院创新基金重大项目623
作者(Author): 侯立安;张伟;杨丹凤;林本成;马力;袭著革;
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参考文献(References)：
- Cao B,Peng J H,Gao N.2001.Studies on acute toxicitymechanism of unsymmetrical dimethylhydrazine onγ-amin obutyricacid receptor[J].Journal of Health Toxicology,5:14-16(inChinese)
- Gu Y P,Guo X Y,Xu L,Xia Y D.1994.The biochemicaleffect of inhaled hydrazine on liver and lung tissue of rats[J].Journal of Health Toxicology,8:15-16(in Chinese)
- Helmers S,Ruland R T,Jacob L N.2004.Epithelioid sarcomaof the thumb associated with hydrazine fuel exposure:a casereport[J].Military Medicine,169:41-44
- Hussain S M,Frazier J M.2001.In vitro toxicity assessment ofa new series of high energy compounds[J].The Science of theTotal Environment,274:151-160
- Hussain S M,Frazier J M.2003.Involvement of apoptosis inhydrazine induced toxicity in rat primary hepatocytes[J].Toxicology in Vitro,17:343-355
- Kao Y H,Chong C H,Ng W T,Lim D.2007.Hydrazineinhalation hepatotoxicity[J].Occupational Medicine,57:535-537
- Li W,Ma Y M,Huang L C,Qin X M,Zhou J,Pan C Y.2002.In situ experimental study on rat injuries induced by liquidpush substanc[J].Chinese Occupational Medicine,29:26-28(in Chinese)
- Makarovsky I,Markel G,Dushnitsky T,Eisenkraft A.2008.Hydrazine—the space era agent[J].The Israel Medical AssociationJournal,10:302-306
- Wu F L,Zhang G Y,Yan X,Hu W X.2001.Advance in theresearch of the analysis and detection technology of hydrazinespropellant[J].Modern Instruments,(6):13-17(in Chinese)
- Yang R,Liu J H,Li S M.2005.Toxicology of unsymmetricaldimethyl hydrazine and protection for exposed staff[J].Journalof Labour Medicine,22:556-558(in Chinese)
- 曹波,彭俊华,高宁.2001.火箭推进剂偏二甲基肼对大鼠γ-氨基丁酸受体的影响[J].卫生毒理学杂志,5:14-16
- 顾依平,郭绪益,徐亮,夏亚东.1994.吸入肼对大鼠肝肺组织某些生化指标的影响[J].卫生毒理学杂志,8:15-16
- 贾庆军,刘天鹏,郭魁亮,冯长龙.2005.液体火箭推进剂的毒理学研究[J].白求恩军医学院学报,3:173-175
- 李伟,马遥明,黄莉纯,秦锡梅,周静,潘春颖.2002.液体推进剂对大鼠机体影响的现场实验研究[J].中国职业医学,29:26-28
- 吴福丽,张光友,延玺,胡文祥.2001.肼类火箭推进剂分析检测技术研究进展[J].现代仪器,(6):13-17
- 杨蓉,刘建华,李松梅.2005.偏二甲肼的毒理和接触人员的安全防护[J].环境与职业医学,22:556-558
- 杨蓉,王煊军.2003.肼类燃料毒性毒理分析及安全防护[J].航天发射技术,4:36-42
- 张捷,刘刚,闺庆旺,李进利,王海涛,姜韧,谭生建.2003.维生素B6在肼类火箭推进剂中毒救治中的应用[J].药学实践杂志,23:53-55