双齿围沙蚕对芘的生物可利用性研究Bioavailablility of Pyrene in the Perinereis aibuhitensis
蔡富才;赵欢;李艳楠;张文龙;杨大佐;周一兵;
摘要(Abstract):
为近海岸沉积质中PAHs的生态风险评价及其污染生境的修复提供理论依据,以双齿围沙蚕为研究对象,以芘为暴露污染物,开展双齿围沙蚕对芘的生物可利用性研究,分别考察了芘对双齿围沙蚕抗氧化酶活性的影响以及芘在双齿围沙蚕体内的生物富集特征。结果表明,双齿围沙蚕SOD、CAT和GSH-PX活性均随诱导时间的增加而升高,且SOD活性随芘浓度的增加出现显著性抑制,CAT和GSH-PX活性则随芘浓度的增加出现显著性升高。芘在双齿围沙蚕体内的生物富集特征如下:双齿围沙蚕体内芘的蓄积量随暴露浓度的增加而增加,各处理组双齿围沙蚕体内芘的蓄积量随时间的变化均呈类凸形的二次曲线关系,在第10天达到蓄积的最大值,最大蓄积量依次为(6.18±0.62)mg·kg~(-1)、(12.37±1.23)mg·kg~(-1)和(24.76±1.51)mg·kg~(-1)。生物富集系数BCF随芘浓度的增加而减小,分别为0.0692、0.0684和0.0674。上述研究结果表明,在一定浓度范围内,双齿围沙蚕抗氧化酶活性对芘的暴露具有很好的指示作用;此外,在短期内,双齿围沙蚕对芘具有明显的富集效应。
关键词(KeyWords): 芘;双齿围沙蚕;生物可利用性;抗氧化酶;生物富集
基金项目(Foundation): 国家自然科学基金资助项目(41306138);; 海洋公益性行业专项(201305002,201305043);; 辽宁省教育厅一般项目(L201608)
作者(Author): 蔡富才;赵欢;李艳楠;张文龙;杨大佐;周一兵;
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DOI:
参考文献(References):
- [1]Tsai W T,Mi H H,Chang Y M,et al.Polycyclic aromatic hydrocarbons(PAHs)in bio-crudes from induction-heating pyrolysis of biomass wastes[J].Bioresource Technology,2007,98(5):1133-1137
- [2]Durant J L,Busby Jr W F,Lafleur A L,et al.Human cell mutagenicity of oxygenated,nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols[J].Mutation Research/Fundamental&Molecular Mechanisms of Mutagenesis,1996,371(3-4):123-157
- [3]Chen Y Y,Zhu L Z,Zhou R B.Characterization and distribution of polycyclic aromatic hydrocarbon in surface water and sediment from Qiantang River,China[J].Journal of Hazardous Materials,2007,141(1):148-155
- [4]梁慧姣,刘小龙,白莉,等.多环芳烃在水生环境食物链中的研究进展[J].安徽农业科学,2014,29:10261-10265Liang H J,Liu X L,Bai L,et al.Research progress of polycyclic aromatic hydrocarbons(PAHs)in food chain of aquatic environment[J].Journal of Anhui Agricultural Sciences,2014,29:10261-10265(in Chinese)
- [5]Haritash A K,Kaushik C P.Biodegradation aspects of polycyclic aromatic hydrocarbons(PAHs):A review[J].Journal of Hazardous Materials,2009,169(1-3):1-15
- [6]Zhang J,Lin X G,Zeng J,et al.In-situ phytoremediation of PAHs contaminateds soils in a mixed cropping system[J].Chinese Journal of Environmental Engineering,2012,6(1):341-346
- [7]Keith L,Telliard W.ES&T special report:Priority pollutants:I-a perspective view[J].Environmental Science&Technology,1979,13(4):416-423
- [8]宋玉芳,周启星,许华夏,等.菲、芘、1,2,4-三氯苯对蚯蚓的急性毒性效应[J].生态与农村环境学报,2003,19(1):36-39Song Y F,Zhou Q X,Xu H X,et al.Effect of acute toxicity of phenanthrene,pyrene and 1,2,4-trichlorobenzene on earthworms in soils[J].Rural Eco-Environment,2003,19(1):36-39(in Chinese)
- [9]吴尔苗,王军良,赵士良,等.菲和芘单一及复合污染对蚯蚓抗氧化酶活性和丙二醛含量的影响[J].环境科学学报,2011,31(5):1077-1085Wu E M,Wang J L,Zhao S L,et al.Effect of single and combined pollution of Phe,Pyr on SOD,CAT activities and MDA content of Eisenia foeada in soils[J].Acta Scientiae Circumstantiae,2011,31(5):1077-1085(in Chinese)
- [10]Lopez G R,Levinton J S.Ecology of deposit-feeding animals in marine sediments[J].The Quarterly Review of Biology,1987,62(3):235-260
- [11]Matozzo V,GagnéF,Marin M G,et al.Vitellogenin as a biomarker of exposure to estrogenic compounds in aquatic invertebrates:A review[J].Environment International,2008,34(4):531-545
- [12]Amiard J C,Geffard A,Amiard-Triquet C,et al.Relationship between the lability of sediment-bound metals(Cd,Cu,Zn)and their bioaccumulation in benthic invertebrates[J].Estuarine Coastal&Shelf Science,2007,72(3):511-521
- [13]Tian Y L,Liu H J,Wang Q X,et al.Acute and chronic toxic effects of Pb2+on polychaete Perinereis aibuhitensis:Morphological changes and responses of the antioxidant system[J].Journal of Environmental Sciences,2014,26(8):1681-1688
- [14]Jrgensen A,Giessing A M,Rasmussen L J,et al.Biotransformation of polycyclic aromatic hydrocarbons in marine polychaetes[J].Marine Environmental Research,2008,65(2):171-186
- [15]崔艳芳,滕彦国,刘晶,等.生物可利用性及其在重金属污染生态风险评价中的作用[J].环境保护科学,2008,34(1):44-46Cui Y F,Teng Y G,Liu J,et al.Bioavailability and its significance for ecological risk assessment of heavy metal pollution[J].Environmental Protection Science,2008,34(1):44-46(in Chinese)
- [16]Juhasz A L,Megharaj M,Naidu R.Bioavailability:The major challenge(constraint)to bioremediation of organically contaminated soils[M]//Wise D L.eds.Bioremediation of Contaminated Soils.New York:Marcel Dekker Inc,2000:217-242
- [17]宋莹莹,袁秀堂,张升利,等.苯并(a)芘、镉单一及复合污染对双齿围沙蚕3刚节疣足幼体发育的影响[J].海洋环境科学,2011,30(3):333-336Song Y Y,Yuan X T,Zhang S L,et al.Single and joint toxic effects of benzo(a)pyrene and cadmium on development of three-setiger juvenile of polychaete Pernereis aibuhitensis Grube[J].Marine Environmental Science,2011,30(3):333-336(in Chinese)
- [18]赵欢,赵新达,岳宗豪,等.苯并(a)芘对双齿围沙蚕抗氧化酶活性和细胞色素P450基因表达的影响[J].大连海洋大学学报,2014(4):342-346Zhao H,Zhao X D,Yue Z H,et al.Effects of B(a)P exposure on antioxidant enzyme activity and CYP450gene expression in sandworm Perinereis aibuhitensis[J].Journal of Dalian Fisheries University,2014(4):342-346(in Chinese)
- [19]Fornari R E,Alessi P,Kikic I.Chem Inform Abstract:High pressure fluid phase equilibria:Experimental methods and systems investigated(1978-1987)[J].Chem Inform,1990,57(47):1-33
- [20]陶宇,蔡富才,赵欢,等.沙蚕对翅碱蓬-降油细菌系统降解菲效果的影响[J].应用与环境生物学报,2016,22(5):891-896Tao Y,Cai F C,Zhao H,et al.Effect of Perinereis aibuhitensis on the degradation of phenanthrene in Suaeda heteroptera and oil-degradation bacteria system[J].Journal of Applied&Environmental Biology,2016,22(5):891-896(in Chinese)
- [21]孙闰霞,柯常亮,林钦,等.超声提取/气相色谱-质谱法测定海洋生物中的多环芳烃[J].分析测试学报,2013,32(1):57-63Sun R X,Ke C L,Lin Q,et al.Determination of polycyclic aromatic hydrocarbons(PAHs)in marine organisms by ultrasonic extraction and gas chromatography-mass spectrometry[J].Journal of Instrumental Analysis,2013,32(1):57-63(in Chinese)
- [22]Phillips D J H.The common mussel Mytilus edulis as an indicator of pollution by zinc,cadmium,lead and copper.I.Effects of environmental variables on uptake of metals[J].Marine Biology,1976,38(1):59-69
- [23]Pastor A,Medina J,Del R J,et al.Determination of lead in treated crayfish Procambarus clarkii:Accumulation in different tissues[J].Bulletin of Environmental Contamination and Toxicology,1988,41(3):412-418
- [24]Papadimitriou E,Loumbourdis N S.Exposure of the frog Rana ridibunda to copper:Impact on two biomarkers,lipid peroxidation,and glutathione[J].Bulletin of Environmental Contamination and Toxicology,2002,69(6):885-891
- [25]Sarkar S,Yadav P,Trivedi R,et al.Cadmium-induced lipid peroxidation and the status of the antioxidant system in rat tissues[J].Journal of Trace Elements in Medicine and Biology,1995,9(3):144-149
- [26]闫博,王兰,李涌泉,等.镉对长江华溪蟹肝胰腺抗氧化酶活力的影响[J].动物学报,2007,53(6):1121-1128Yan B,Wang L,Li Y Q,et al.Effects of cadmium on hepatopancreatic antioxidant enzyme activity in a freshwater crab Sinopotamon yangtsekiense[J].Current Zoology,2007,53(6):1121-1128(in Chinese)
- [27]SoléM,Porte C,Albaigés J.Mixed-function oxygenase system components and antioxidant enzymes in different marine bivalves:Its relation with contaminant body burdens[J].Aquatic Toxicology,1994,30(3):271-283
- [28]Mourente G,Daz-Salvago E,Bell J G,et al.Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream(Sparus aurata L.)fed dietary oxidised oil:Attenuation by dietary vitamin E[J].Aquaculture,2002,214(1):343-361
- [29]任加云,潘鲁青,苗晶晶.苯并(a)芘和苯并(k)荧蒽混合物对栉孔扇贝毒理学指标的影响[J].环境科学学报,2006,26(7):1180-1186Ren J Y,Pan L Q,Miao J J.Effects of benzo(a)pyrene and benzo(k)fluoranthene mixture on the toxicology parameter of scallop Chlamys farreri[J].Acta Scientiae Circumstantiae,2006,26(7):1180-1186(in Chinese)
- [30]丁正峰,薛晖,王晓丰,等.乐斯本对克氏原螯虾肝胰脏抗氧化系统的影响[J].大连海洋大学学报,2013,28(1):72-76Ding Z F,Xue H,Wang X F,et al.Effects of Lorsban on antioxidant system in hepatopancreas of red swamp crayfish Procambarus clarkii[J].Journal of Dalian Fisheries University,2013,28(1):72-76(in Chinese)
- [31]Livingstone D,Archibald S,Chipman J,et al.Antioxidant enzymes in liver of dab Limanda limanda from the North Sea[J].Marine Ecology Progress,1992,91(1-3):97-104
- [32]陶宇.沙蚕对翅碱蓬-降油细菌组合降解三种多环芳烃效果的影响[D].大连:大连海洋大学,2016:15-17Tao Y.Effect of Perinereis aibuhitensis on the degradation of three kinds of PAHs in Suaeda heteroptera and oildegradation bacteria models[D].Dalian:Dalian Ocean University,2016:15-17(in Chinese)
- [33]Meador J P,Ernest D W,Kagley A N.A comparison of the non-essential elements cadmium,mercury,and lead found in fish and sediment from Alaska and California[J].Science of The Total Environment,2005,339(1-3):189-205
- [34]薛秋红,孙耀.紫贻贝对石油烃的生物富集动力学参数的测定[J].渔业科学进展,2001,22(1):32-36Xue Q H,Sun Y.Determination of kinetic parameters for bioconcentration of petroleum hydrocar bons by Mytilus edulis[J].Marine Fisherics Research,2001,22(1):32-36(in Chinese)
- [35]姜朝军,乔庆林,蔡友琼,等.菲律宾蛤仔对石油烃的污染动力学和阈值研究[J].海洋渔业,2006,28(4):314-320Jiang C J,Qiao Q L,Cai Y Q,et al.Kinetic features and threshold value of petroleum hydrocarbons for Ruditapes philippinarum[J].Marine Fisheries,2006,28(4):314-320(in Chinese)