张雅晶;缪恒锋;张晓夏;阮文权;

• 1:江南大学环境与土木工程学院
• 2:江苏省厌氧生物技术重点实验室
• 3:江苏省水处理技术与材料协同创新中心

摘要(Abstract)：

为了探究消毒副产物(DBPs)的氧化损伤和神经毒性作用,选择幼年斑马鱼作为模式生物,研究了市政污水处理厂二级出水和经次氯酸钠(NaClO)消毒后的二级出水对斑马鱼体内过氧化氢酶(CAT)活性、丙二醛(MDA)含量和乙酰胆碱酯酶(Ach E)活性的影响。结果表明,暴露时间达到10 d时,MDA含量显著高于对照,且消毒后MDA含量增幅(91.43%)显著高于消毒前(44.36%);暴露时间达到15 d时,CAT活性被显著抑制,且消毒后抑制率(40.22%)显著高于消毒前(15.56%);说明消毒后暴露组对斑马鱼的氧化损伤强于消毒前。另外,消毒前后污水对Ach E活性抑制率分别为38.49%和48.50%,说明消毒后污水对斑马鱼的神经毒性更大。因此,经NaClO消毒后的市政污水处理厂二级出水中DBPs对斑马鱼的抗氧化防御系统和神经系统具有潜在影响。

关键词(KeyWords)： 消毒副产物;幼年斑马鱼;氧化损伤;神经毒性;联合毒性

Abstract:

Keywords:

基金项目(Foundation): 水体污染控制与治理科技重大专项(2017ZX07203-003)

作者(Author): 张雅晶;缪恒锋;张晓夏;阮文权;

Email:

参考文献(References)：

• [1]李亮,黄种买,张传国.UASB-SBR工艺处理城市生活污水的研究[J].工业水处理,2005,25(10):21-23Li L,Huang Z M,Zhang C G.Application of UASB-SBRprocess to the domestic sewage treatment[J].Industrial Water Treatment,2005,25(10):21-23(in Chinese)
• [2]Liu J L,Li X Y,Xie Y F,et al.Characterization of soluble microbial products as precursors of disinfection byproducts in drinking water supply[J].Science of the Total Environment,2014,472:818-824
• [3]Fukushima T,Hara-Yamamura H,Urai M,et al.Toxicity assessment of chlorinated wastewater effluents by using transcriptome-based bioassays and Fourier transform mass spectrometry(FT-MS)analysis[J].Water Research,2014,52:73-82
• [4]罗妮娜.新型消毒副产物碘代乙酸急性暴露诱导斑马鱼肝脏氧化损伤的机理研究[J].环境科学学报,2016,36(8):3088-3094Luo N N.Oxidative damage mechanism ofzebrafish(Danio rerio)liver tissue after acute exposure to iodine acetic acid in vivo[J].Acta Scientiae Circumstantiae,2016,36(8):3088-3094(in Chinese)
• [5]Yang M T,Zhang X R.Comparative developmental toxicity of new aromatic halogenated DBPs in a chlorinated saline sewage effluent to the marine polychaete Platynereis dumerilii[J].Environmental Science&Technology,2013,47(19):10868-10876
• [6]Krasner S W,Weinberg H S,Richardson S D,et al.The occurrence of a new generation of disinfection byproducts[J].Environmental Science&Technology,2016,40(23):7175-7185
• [7]da Costa J B,Rodgher S,Daniel L A,et al.Toxicity on aquatic organisms exposed to secondary effluent disinfected with chlorine,peracetic acid,ozone and UV radiation[J].Ecotoxicology,2014,23:1803-1813
• [8]李丽君,刘振乾,徐国栋,等.工业废水的鱼类急性毒性效应研究[J].生态科学,2006,25(1):43-47Li L J,Liu Z Q,Xu G D,et al.Study on acute-toxicity of six kind industrial wastewaters to zebrafish[J].Ecologic Science,2006,25(1):43-47(in Chinese)
• [9]李敏,赵月,曹志会,等.链霉素废水对斑马鱼POD活性和GSH含量的影响[J].安全与环境工程,2015,22(3):1-5,11Li M,Zhao Y,Cao Z H,et al.Impact of streptomycin wastewater on the activity of peroxidase(POD)and the content of glutathione(GSH)in the muscle tissue of Danio rerio[J].Safety and Environmental Engineering,2015,22(3):1-5,11(in Chinese)
• [10]Shi L,Wang D,Cao D,et al.Is A/A/O process effective in toxicity removal?Case study with coking wastewater[J].Ecotoxicology and Environmental Safety,2017,142:363-368
• [11]吉贵祥,石利利,刘济宁,等.BDE-47对斑马鱼胚胎-幼鱼的急性毒性及氧化应激作用[J].生态毒理学报,2013,8(5):731-736Ji G X,Shi L L,Liu J N,et al.Acute toxicity and oxidative stress effects of BDE-47 on zebrafish embryos and larvae[J].Asian Journal of Ecotoxicology,2013,8(5):731-736(in Chinese)
• [12]何桢,牟文,周巧巧,等.Hg Cl2对斑马鱼SOD,ACh E酶活性和基因相对转录量的影响[J].生态毒理学报,2011,6(6):649-654He Z,Mou W,Zhou QQ,et al.Toxic effects of Hg Cl2on SOD and ACh E of activities and gene transcription of zebrafish[J].Asian Journal of Ecotoxicology,2011,6(6):649-654(in Chinese)
• [13]张元园.氧化乐果对斑马鱼的毒性作用[D].青岛:青岛科技大学,2016:10-12Zhang Y Y.Toxicity effect of omethoate toDanio rerio[D].Qingdao:Qingdao University of Technology,2016:10-12(in Chinese)
• [14]王隽媛,边红枫,金香琴,等.萘对斑马鱼(Danio rerio)内脏团抗氧化防御系统的胁迫与生物响应[J].环境科学,2009,30(2):516-521Wang J Y,Bian H F,Jin X Q,et al.Stress and biological response of naphthalene on the antioxidant defense system in visceral mass of zebrafish(Danio rerio)[J].Environmental Science,2009,30(2):516-521(in Chinese)
• [15]国家环境保护总局.水和废水监测分析方法4版[M].北京:中国环境科学出版社,2002:请补充引用页码
• [16]Bronk D A,Lomas M W,Glibert P M,et al.Total dissolved nitrogen analysis:Comparisons between the persulfate,UV and high temperature oxidation methods[J].Marine Chemistry,2000,69(1-2):163-178
• [17]张赛,胡学斌,古励,等.高藻水臭氧预氧化过程有机物转化及消毒副产物生成势[J].环境科学,2017,38(3):1038-1045Zhang S,Hu X B,Gu L,et al.Peroxidation of high algaeladen water by ozone:Algae organic matter transformation and disinfection by-products formation[J].Environmental Science,2017,38(3):1038-1045(in Chinese)
• [18]中华人民共和国环境保护部.HJ 585-2010水质游离氯和总氯的测定N,N-二乙基1,4-苯二胺滴定法[S].北京:中国环境科学出版社,2010
• [19]US EPA.Method 551.Determination of chlorination disinfection byproducts and chlorinated solvents in drinking water by liquid-liquid extraction and gas chromatography with electron-capture detection[S].Washinton DC:USEPA,1990
• [20]US EPA.Method 552.3.Determination of haloacetic acids and dalapon in drinking water by liquid-liquid microextraction,derivatization,and gas chromatography with electron capture detection[S].Washinton DC:USEPA,2003
• [21]国家环境保护总局.HJ/T83-2001水质可吸附有机卤素(AOX)的测定离子色谱法[S].北京:中国标准出版社,2001
• [22]中华人民共和国卫生部.中国国家标准化管理委员会.GB 5749-2006.生活饮用水卫生标准[S].北京:中国标准出版社,2006
• [23]World Health Organization.Guidelines for drinking-water quality[S].Geneva:World Health Organization,2011
• [24]Woo Y T,Lai D,McLain J L,et al.Use of mechanismbased structure-activity relationship analysis incarcinogenic potential ranking for drinking water disinfection by-products[J].Environmental Health Perspectives,2002,110(1):75-87
• [25]江瑞,黄璜,吴乾元,等.再生水氯消毒过程中三氯丙酮生成特性及其前体物组成[J].给水排水,2013(S1):171-176Jiang R,Huang H,Wu Q Y,et al.Trichloroacetone formation from the chlorination of reclaimed water and the characteristic of its precursor component[J].Water&Wastewater Engineering,2013(S1):171-176(in Chinese)
• [26]杨帆,楚文海,张永吉,等.饮用水有机类消毒副产物毒理学研究方法进展[J].生态毒理学报,2012,7(1):35-43Yang F,Chu W H,Zhang Y J,et al.Recent advances in toxicological methods for testing toxicity of organic disinfection by-products(DBPs)in drinking water[J].Asian Journal of Ecotoxicology,2012,7(1):35-43(in Chinese)
• [27]Zhang B B,Xian Q M,Zhu J P,et al.Characterization,DBPs formation,and mutagenicity of soluble microbial products(SMPs)in wastewater under simulated stressful conditions[J].Chemical Engineering Journal,2015,279:258-263
• [28]Lin T,Zhou D J,Dong J,et al.Acute toxicity of dichloroacetonitrile(DCAN),a typical nitrogenous disinfection by-product(N-DBP),on zebrafish(Danio rerio)[J].Ecotoxicology and Environmental Safety,2016,133:97-104
• [29]Zheng J,Lin T,Chen W.Removal of the precursors of N-nitrosodiethylamine(NDEA),an emerging disinfection byproduct,in drinking water treatment process and its toxicity to adult zebrafish(Danio rerio)[J].Chemosphere,2018,191:1028-1037
• [30]Yu S L,Lin T,Chen W,et al.The toxicity of a new disinfection by-product,2,2-dichloroacetamide(DCA-c Am),on adult zebrafish(Danio rerio)and its occurrence in the chlorinated drinking water[J].Chemosphere,2015,139:40-46
• [31]Gong T T,Zhang X R,Li Y,et al.Formation and toxicity of halogenated disinfection byproducts resulting from linear alkylbenzene sulfonates[J].Chemosphere,2016,149:70-75
• [32]Yang M M,Liu J Q,Zhang X R,et al.Comparative toxicity of chlorinated saline and freshwater wastewater effluents to marine organisms[J].Environmental Science&Technology,2015,49(24):14475-14483
• [33]Tao L T,Zhou D G,Yu S L,et al.The removal process of 2,2-dichloroacetamide(DCAc Am),a new disinfection by-product,in drinking water treatment process and its toxicity on zebrafish[J].Chemosphere,2016,159:403-411
• [34]汪鹏鹏,张松林,靳佳,等.辛硫磷和敌百虫协同抑制斑马鱼乙酰胆碱酯酶(ACh E)活性[J].生态毒理学报,2017,12(1):298-304Wang P P,Zhang S L,Jin J,et al.Phoxim and trichlorfon synergistically reduce acetylcholinesterase(Ach E)activity of zebrafish(Brachydanio rerio)[J].Asian Journal of Ecotoxicology,2017,12(1):298-304(in Chinese)