刘晓晖;李振伟;刘畅;朱春艳;钱波;邵静;胡宏;

• 1:大连医科大学公共卫生学院劳动卫生与环境卫生教研室
• 2:大连医科大学附属二院检验科

摘要(Abstract)：

为了研究新型持久性有机污染物全氟辛烷磺酸(peruorooctane sulfonate,PFOS)的神经毒性及其机制,将PC12细胞暴露于31.25、62.5、125、250、500μmol·L-1的PFOS,利用噻唑蓝(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT)法检测细胞存活率、二氯二氢荧光素-乙酰乙酸酯(2’,7’-Dichlorofluorescin diacetate,DCFH-DA)荧光探针检测活性氧含量、Hoechst 33342/PI试剂盒检测细胞凋亡及坏死情况、溶酶体特异性荧光染料Lyso-Tracker Red监测细胞内溶酶体数量;利用实时荧光定量PCR(quantitative real-time reverse transcription-polymerase chain reaction,q RT-PCR)技术对自噬发生的代表性基因Atg5、Atg 12、Beclin 1的表达情况进行考察。结果发现,与对照组比较,250、500μmol·L-1PFOS显著降低PC12细胞存活率(P<0.05);62.5、125、250、500μmol·L-1PFOS诱导活性氧含量升高(P<0.05);62.5、125、250、500μmol·L-1PFOS诱导PC12细胞凋亡(P<0.05);125、250、500μmol·L-1PFOS升高溶酶体数量及Atg 5、Atg 12、Beclin 1基因表达(P<0.05)。结果表明,PFOS能够诱导PC12细胞死亡,其机制可能为干扰自噬与凋亡过程。

关键词(KeyWords)： PFOS;PC12;神经毒性;凋亡;自噬

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81273031;81302400);; 大连理工大学工业生态与环境工程教育部重点实验室开放基金(KLIEEE-12-04)

作者(Author): 刘晓晖;李振伟;刘畅;朱春艳;钱波;邵静;胡宏;

Email:

DOI:

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