斜带石斑鱼alpha型与rho型GST基因cDNA全序列的克隆与分析Cloning and Sequence Analysis of the Alpha and Rho-Class GST Genes in Orange-Spotted Grouper (Epinephelus coioides)
胡永乐;梁旭方;林群;李光照;李观贵;王琳;
摘要(Abstract)：
为从分子水平上研究II相去毒酶谷胱甘肽S-转移酶(Glutathione S-transferase,GST)在斜带石斑鱼(Epinephelus coioides)等海水鱼类机体内代谢去毒过程中的作用及机制,采用RT-PCR及RACE法,分离、克隆得到斜带石斑鱼肝脏alpha-GST(GSTA)、rho-GST(GSTR)基因cDNA全序列.序列分析结果表明,斜带石斑鱼肝脏GSTA基因cDNA全长1008bp,编码223个氨基酸;GSTR基因cDNA全长1002bp,编码225个氨基酸.构建系统进化树发现,斜带石斑鱼与鲤鱼、斑马鱼GSTA氨基酸同源性较高,而GSTR为鱼类所特有并共同占据进化树上独立的分枝,与大鼠、小鼠、人等哺乳动物GST现有所有类型同源性均较低.
关键词(KeyWords)： 斜带石斑鱼;谷胱甘肽S-转移酶;基因克隆;序列分析
基金项目(Foundation): 国家科技部863项目(No. 2007AA09Z437);; 国家自然科学基金项目(No. 30670367);; 广东省科技计划项目(No. 2007B020701002);; 广东省科技计划项目(No. 2005B20301005);; 广州市科技计划(No. 2006Z3-E0551);; 教育部留学回国人员科研启动基金项目
作者(Author): 胡永乐;梁旭方;林群;李光照;李观贵;王琳;
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DOI:
参考文献(References)：
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