全氟辛酸对斜生栅藻细胞膜特性影响研究Effect of Perfluorooctane Acid on Cell Membrane Properties of Scenedesmus obliquus
冯政;郑新梅;刘红玲;龚伊;于洋;于红霞;
摘要(Abstract)：
斜生栅藻暴露于全氟辛酸(PFOA)中,其细胞膜特性可通过不同荧光染料染色的荧光信号变化来反映.应用流式细胞仪检测荧光信号,研究了PFOA对斜生栅藻细胞膜的完整性、选择透过性和膜电位指标的影响效应.实验结果显示,全氟辛酸对表征细胞膜完整性的碘化丙啶(PI)染料荧光强度起到显著刺激作用,栅藻细胞膜受损几率随暴露浓度升高呈现上升趋势;低暴露浓度下栅藻细胞膜选择透过性增强,但在高浓度暴露下这一细胞膜特性受到明显抑制,具体表现为表征酯酶活性的二乙酸荧光素(FDA)荧光强度出现先升后降的变化;与FDA荧光强度变化趋势相反,反映膜电位的碘代3,3’-二己氧基羰花青(DIOC6(3))荧光强度则先降后升,预示高浓度PFOA暴露将导致细胞膜处于异常的生理状态.
关键词(KeyWords)： 全氟辛酸;斜生栅藻;流式细胞术;细胞膜
基金项目(Foundation): 国家科技重大专项资助(No.2008ZX08526-003);; 江苏省省级环保科技项目(No.2008005);; 江苏省环境监测科研基金项目(No.0809)
作者(Author): 冯政;郑新梅;刘红玲;龚伊;于洋;于红霞;
Email:
参考文献(References)：
- Dietz R,Bossi,R,Riget,F F,Sonne C,Born E W.2008.Increasing perfluoroalkyl contaminants in east Greenland polarbears(Ursus maritimus):A new toxic threat to the Arctic bears[J].Environmental Science&Technology,42(7):2701-2707
- Hansen K J,Johnson H O,Eldridge J S,Butenhoff J L,Dick LA.2002.Quantitative characterization of trace levels of PFOSand PFOA in the Tennessee River[J].Environmental Science&Technology,36(8):1681-1685
- Ji K,Kim Y,Oh S,Ahn B,Jo H,Choi K.2008.Toxicity ofperfluorooctane sulfonic acid and perfluorooctanoic acid onfreshwater macroinvertebrates(Daphnia magna and Moinamacrocopa)and fish(Oryzias latipes)[J].EnvironmentalToxicology and Chemistry,27(10):2159-2168
- Jones P D,Hu W,De Coen W,Newsted J L,Giesy J P.2003.Binding of Perfluorinated fatty acids to serum proteins[J].Environmental Toxicology and Chemistry,22(11):2639-2649
- Lien N P H,Fujii S,Tanaka S,Nozoe M,Tanaka H.2008.Contamination of perfluorooctane sulfonate(PFOS)andperfluorooctanoate(PFOA)in surface water of the Yodo Riverbasin(Japan)[J].Desalination,226(1-3):338-347
- Loos R,Wollgast J,Huber T,Hanke G.2007.Polar herbicides,pharmaceutical products,perfluorooctanesulfonate(PFOS),perfluorooctanoate(PFOA),and nonylphenol and its carboxylatesand ethoxylates in surface and tap waters around Lake Maggiorein Northern Italy[J].Analytical and Bioanalytical Chemistry,387(4):1469-1478
- Nakayama S,Harada K,Inoue K,Sasaki K,Seery B,Saito N,Koizumi A.2005.Distributions of perfluorooctanoic acid(PFOA)and perfluorooctane sulfonate(PFOS)in Japan and their toxicities[J].Environmental Science&Technology,12(6):293-313
- Pistocchi A,Loos R.2009.A map of European emissions andconcentrations of PFOS and PFOA[J].Environmental Science&Technology,43(24):9237-9244
- Qazi M R,Bogdanska J.2009.High-dose,short-term exposureof mice to perfluorooctanesulfonate(PFOS)or perfluorooctanoate(PFOA)affects the number of circulating neutrophils differently,but enhances the inflammatory responses of macrophages tolipopolysaccharide(LPS)in a similar fashion[J].Toxicology,262(3):207-214
- Skutlarek D,Exner M,Farber H.2006.Perfluorinated surfactantsin surface and drinking water[J].Environmental Science andPollution Research,13(5):299-307
- Wang Y,Yeung L W Y,Yamashita N,Taniyasu S,So M K,Murphy M B,Lam P K S.2008.Perfluorooctane sulfonate(PFOS)and related fluorochemicals in chicken egg in China[J].Chinese Science Bulletin,53(4):501-507
- Ye L,Wu L L,Jiang Y X,Zhang C J,Chen L.2009.Toxicological study of PFOS/PFOA to Zebrafish(Brachydanio rerio)embryos[J].Environmental Science,30(6):1727-1732(in Chinese)
- Yeung L W Y,Guruge K S.2007.Differential expression ofchicken hepatic genes responsive to PFOA and PFOS[J].Toxicology,237(1-3):111-125
- Yu J,Hu J Y.2009.Perfluorooctane sulfonate(PFOS)andperfluorooctanoic acid(PFOA)in sewage treatment plants[J]Water Research,43(9):2399-2408
- Yu Y,Kong F X,Wang M L,Shi X L.2006.Effect of copperon membrane integrality and esterase activity of microcystisaeruginosa using flow cytometry[J].Chinese Journal of Applied&Environmental Biology,12(5):706-709(in Chinese)
- 叶露,吴玲玲,蒋雨希,张超杰,陈玲.2009.PFOS/PFOA对斑马鱼(Brachydanio rerio)胚胎致毒效应研究[J].环境科学,30(6):1727-1732
- 于洋,孔繁翔,王美林,史小丽.2006.应用流式细胞技术研究铜对藻细胞膜完整性及脂酶活性的影响[J].应用与环境生物学报,12(5):706-709