全氟辛酸对斜生栅藻细胞膜特性影响研究Effect of Perfluorooctane Acid on Cell Membrane Properties of Scenedesmus obliquus
冯政;郑新梅;刘红玲;龚伊;于洋;于红霞;
摘要(Abstract)：
斜生栅藻暴露于全氟辛酸(PFOA)中,其细胞膜特性可通过不同荧光染料染色的荧光信号变化来反映.应用流式细胞仪检测荧光信号,研究了PFOA对斜生栅藻细胞膜的完整性、选择透过性和膜电位指标的影响效应.实验结果显示,全氟辛酸对表征细胞膜完整性的碘化丙啶(PI)染料荧光强度起到显著刺激作用,栅藻细胞膜受损几率随暴露浓度升高呈现上升趋势;低暴露浓度下栅藻细胞膜选择透过性增强,但在高浓度暴露下这一细胞膜特性受到明显抑制,具体表现为表征酯酶活性的二乙酸荧光素(FDA)荧光强度出现先升后降的变化;与FDA荧光强度变化趋势相反,反映膜电位的碘代3,3’-二己氧基羰花青(DIOC6(3))荧光强度则先降后升,预示高浓度PFOA暴露将导致细胞膜处于异常的生理状态.
关键词(KeyWords)： 全氟辛酸;斜生栅藻;流式细胞术;细胞膜
基金项目(Foundation): 国家科技重大专项资助(No.2008ZX08526-003);; 江苏省省级环保科技项目(No.2008005);; 江苏省环境监测科研基金项目(No.0809)
作者(Author): 冯政;郑新梅;刘红玲;龚伊;于洋;于红霞;
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DOI:
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