王晓艳;蒋凤华;冯丽娟;高伟;赵美丽;郑立;

• 1:中国海洋大学化学化工学院
• 2:国家海洋局第一海洋研究所海洋生态研究中心
• 3:国家海洋局活性物质与现代分析技术重点实验室

摘要(Abstract)：

为研究石油烃对海洋生物的毒性效应,将栉孔扇贝(Chlamys farreri)暴露于0.08、0.21和0.88mg·L-1石油烃中,采用单细胞凝胶电泳实验(彗星实验)技术检测不同暴露时间扇贝血淋巴细胞的DNA损伤程度,对照组中石油烃背景浓度为0.04mg·L-1。结果显示,低浓度(0.08mg·L-1)的石油烃短期(<7d)内即可导致栉孔扇贝血淋巴细胞的DNA损伤,并且随石油烃浓度的增大和暴露时间的延长,DNA损伤程度增加,石油烃浓度达0.88mg·L-1时,DNA损伤程度已非常严重。3d恢复实验后,各浓度组DNA损伤又均有不同程度的恢复。研究表明,彗星实验是检测石油烃对海洋贝类DNA损伤的一种有效手段,贝类血淋巴细胞DNA损伤有望成为石油烃污染的一种生物标志物,用于海洋污染的早期预警监测。

关键词(KeyWords)： 石油烃;栉孔扇贝;血淋巴细胞;DNA损伤;单细胞凝胶电泳;彗星实验

Abstract:

Keywords:

基金项目(Foundation): 海洋公益性科研专项(200705011;200805039;201105013);; 国家海洋局第一海洋研究所基本科研业务费项目(2010T04);国家海洋局青年海洋科学基金项目(2012534)

作者(Author): 王晓艳;蒋凤华;冯丽娟;高伟;赵美丽;郑立;

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DOI:

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