荧光法监测硝普钠释放NO过程及其对微藻生长的影响Fluorescence Dynamic Monitoring of Nitric Oxide Release Process Following Administration of Sodium Nitroprusside and Its Effect on the Growth of Microalga
刘春颖;房爽;赵敏;白效静;韩洋;李培峰;
摘要(Abstract)：
以硝普钠(Sodium nitroprusside,SNP)为一氧化氮(Nitric oxide,NO)供体研究了NO对海洋微藻生长的影响.对不同浓度SNP在海水介质中释放NO的过程进行了监测;对所培养的亚心形扁藻(Platymonas subcordiformis)和中肋骨条藻(Skeletonema costatum)进行藻密度测定,观测NO对微藻生长的影响.结果表明:5、10和100μmol·L-1的SNP释放NO浓度大约分别为6×10-9、9×10-9和2×10-7mol·L-1左右,而释放时间分别为4、5.5和7.5h.研究表明,NO对不同微藻有明显不同的作用规律:NO持续作用下,对亚心形扁藻的最佳作用浓度在10-8mol·L-1数量级;对赤潮藻中肋骨条藻的最佳作用浓度在10-9mol·L-1数量级;赤潮藻对NO的响应比非赤潮藻更灵敏,NO可能是海洋生态系中微藻生长重要的调节因子.
关键词(KeyWords)： 一氧化氮;微藻;硝普钠;荧光法
基金项目(Foundation): 国家自然科学基金(No.40706040;No.40376022);; 山东省中青年科学家科研奖励基金(No.2007BS08015);; 山东省自然科学基金(No.Y2008E04)
作者(Author): 刘春颖;房爽;赵敏;白效静;韩洋;李培峰;
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参考文献(References)：
- A-H-Mackerness S,John C F,Jordan B,Thomas B.2001.Early signaling components in ultraviolet-B responses:distinctroles for different reactive oxygen species and nitric oxide[J].FEBS Letters,489(2-3):237-242
- Ferrer M A,Ros BarcelóA..1999.Differential effects of nitricoxide on peroxidase and H2O2production by the xylem of Zinniaelegans[J].Plant,Cell&Environment,22(7):891-897
- Gow A J,Ischiropoulos H.2001.Nitric oxide chemistry andcellular signaling[J].Journal of Cellular Physiology,187(3):277-282
- Guan Y Y,Lin M J.1999.The role of NO in human immunitysystem[J].New Medicine,1:11-22
- Guo F Q,Okamoto M,Crawford N M.2003.Identification of aplant nitric oxide synthase gene involved in hormonal signaling[J].Science,302(5642):100-103
- Ignarro L J,Buga G M,Wood K S Byrns R E,Chaudhuri G.1987.Endothelium-derived relaxing factor produced and releasedfrom artery and vein is nitric oxide[J].Proceedings of NationalAcademy of Sciences of the United States of America,84(24):9265-9269
- Kopyra M G,Gwóz'dz'E A.2003.Nitric oxide stimulates seedgermination and counteracts the inhibitory effect of heavy metalsand salinity on root growth of Lupinus liteus[J].PlantPhysiology and Biochemistry,41(11-12):1011-1017
- Liu C Y.2006.Studies on the effect laws of nitric oxide on thephytoplankton growth in seawater[D].Qingdao:Ocean Universityof China,209(in Chinese)
- Liu C Y,Zhang Z B,Chen X R.2005.Mutual effects of nitricoxide and iron on the growth of marine algae[J].ActaOceanolagica Sinica,24(5):100-109
- Liu C Y,Zhang Z B,Li P F,Huang H W.2006.Growtheffect of exogenous nitric oxide on Platymonas subcordiformisand spectrum study[J].Environmental Science,27(6):1062-1067(in Chinese)
- Mallick N,Mohn F H,Soeder C J.2000.Impact ofphysiological stresses on nitric oxide formation by green alga,Scenedesnues obligus[J].Microbiology and Biotechnology,110(2):300-307
- Mayer B,Hemmens B.1997.Biosynthesis and action of nitricoxide inmammalian cells[J].Trends in Biochemical Sciences,22(12):477-481
- Palmer R M J,Ferrige A G,Moncada S.1987.Nitric oxiderelease accounts for the biological activity of endothelium-derlvedrelaxing factor[J].Nature,327:524-526
- Raven J A.1997.The role of marine biota in the evolution ofterrestrial biota:Gases and genes[J].Biogeochemistry,39(2):139-164
- Soeder C J,Mohn F H,Pappke H,Rockel P.1996.Scenedesmuscanproduce nitric oxide(NO)[Z].Abstracts of the First EuropeanPhycological Congress,Cologne,July1996,p.52.Cambridge University Press
- Yamasaki H,Sakihama Y,Takahashi S.1999.An alternativepathway for nitric oxide production in plants:new features of anold enzyme[J].Trends in Plant Science,4(4):128-1291
- Zafiriou O C,McFarland M,Bromund R H.1980.Nitric oxidein seawater[J].Science,207(4431):637-639
- Zhang Z B,Lin C,Liu C Y,Xing L,Wu Z Z,Sun F.2005.Study on patterns and chemical features of NO effect on marinephytoplankton growth[J].Science in China Series B:(Chemistry),48(4):376-384
- Zhang Z B,Xing L,Wu Z Z,Liu C Y,Lin C,Liu L S.2006.Discovery of nitric oxide in marine ecological system andthe chemical characteristics of nitric oxide[J].Science in ChinaSeries B:(Chemistry),49(5):475-480
- Zhang Z B,Lin C,Liu C Y,Sun M Y,Ding H B.2003.Theeffect of nitric oxide on the growth of marine phytoplankton[J].Journal of Ocean University of Qingdao,2(2):185-1881
- 刘春颖.2006.海水中一氧化氮对浮游植物生长影响的规律研究[D].青岛:中国海洋大学,209
- 刘春颖,张正斌,李培峰,皇华伟.2006.外源一氧化氮对亚心形扁藻的生长效应及光谱研究[J].环境科学,27(6):1062-1067