荧光法监测硝普钠释放NO过程及其对微藻生长的影响Fluorescence Dynamic Monitoring of Nitric Oxide Release Process Following Administration of Sodium Nitroprusside and Its Effect on the Growth of Microalga
刘春颖;房爽;赵敏;白效静;韩洋;李培峰;
摘要(Abstract)：
以硝普钠(Sodium nitroprusside,SNP)为一氧化氮(Nitric oxide,NO)供体研究了NO对海洋微藻生长的影响.对不同浓度SNP在海水介质中释放NO的过程进行了监测;对所培养的亚心形扁藻(Platymonas subcordiformis)和中肋骨条藻(Skeletonema costatum)进行藻密度测定,观测NO对微藻生长的影响.结果表明:5、10和100μmol·L-1的SNP释放NO浓度大约分别为6×10-9、9×10-9和2×10-7mol·L-1左右,而释放时间分别为4、5.5和7.5h.研究表明,NO对不同微藻有明显不同的作用规律:NO持续作用下,对亚心形扁藻的最佳作用浓度在10-8mol·L-1数量级;对赤潮藻中肋骨条藻的最佳作用浓度在10-9mol·L-1数量级;赤潮藻对NO的响应比非赤潮藻更灵敏,NO可能是海洋生态系中微藻生长重要的调节因子.
关键词(KeyWords)： 一氧化氮;微藻;硝普钠;荧光法
基金项目(Foundation): 国家自然科学基金(No.40706040;No.40376022);; 山东省中青年科学家科研奖励基金(No.2007BS08015);; 山东省自然科学基金(No.Y2008E04)
作者(Author): 刘春颖;房爽;赵敏;白效静;韩洋;李培峰;
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