曹璐璐;李斐;吴惠丰;赵建民;

• 1:中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室
• 2:中国科学院大学

摘要(Abstract)：

2,2’,4,4’-四溴联苯醚(BDE-47)是生物体中含量最高且毒性最强的PBDEs之一,有关BDE-47对肾细胞的毒性及其作用机制的研究仍有待补充。选取3个剂量组(低:10-6mol·L-1、中:10-5mol·L-1、高:10-4mol·L-1)及溶剂对照组,研究了BDE-47对人胚肾细胞(HEK293)的细胞凋亡率及活性氧(ROS)水平的影响;并从分子水平对细胞氧化损伤、凋亡相关蛋白(APE1及p53)及凋亡相关基因m RNA(p53、Bax、Caspase 3、Caspase 8)的表达量进行测定。实验结果显示:与对照组相比,中、高剂量组细胞凋亡率显著增加(P<0.05);ROS水平在中剂量组显著上升(P<0.01);随BDE-47浓度的变化,APE1蛋白表达量与细胞ROS水平存在一致性;p53、Bax、Caspase 8 m RNA表达量与BDE-47的浓度间存在剂量-效应关系。结果表明,BDE-47可诱导HEK293细胞凋亡及氧化应激,APE1可能是细胞ROS升高与细胞凋亡间重要的中介因子;BDE-47可以通过影响Caspase 8及线粒体途径中p53及Bax的表达诱导细胞凋亡。

关键词(KeyWords)： 2,2’,4,4’-四溴联苯醚(BDE-47);人胚肾细胞;细胞凋亡;氧化损伤;p53;Bax

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21107136);; 国际科学基金(F/5230-1);; 中国科学院重点部署项目(KZZD-EW-14);; 中国科学院战略性先导科技专项(A类)(XDA11020405)

作者(Author): 曹璐璐;李斐;吴惠丰;赵建民;

Email:

DOI:

参考文献(References)：

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