张弛;闫会萍;陆一帆;翁凯翔;武铁男;胡兵;王娟娟;刘一凝;魏东洋;许振成;

• 1:北京体育大学运动康复系
• 2:环境保护部华南环境科学研究所

摘要(Abstract)：

观察8周中等强度游泳运动对2,3,7,8-四氯二苯并二恶英(2,3,7,8-tetrachlorodibenzo-p-dioxin,2,3,7,8-TCDD)急性暴露大鼠肝脏氧化应激的影响。以8周龄雄性Sprague Dawley大鼠为研究对象,将大鼠随机分为玉米油静养组(NC组)、玉米油运动组(EC组)、TCDD静养组(NT)和TCDD运动组(ET组)。将TCDD溶于玉米油中,NT和ET组大鼠按照10μg·kg-1(以单位体重计)腹腔注射TCDD,NC和EC组大鼠注射等量玉米油。正式实验开始后,EC和ET组大鼠进行运动(尾部负重5%游泳30min),每周运动5 d,共8周,NC和NT组大鼠不进行任何运动干预。8周后,称重并宰杀大鼠,收集血清和肝组织样本,待测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的活性;肝组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)以及谷胱甘肽过氧化物酶(GSH-Px)的活性。将数据进行多因素方差分析,结果表明,染毒可升高大鼠血清AST的活性,增加肝脏MDA的含量,降低肝脏SOD、CAT和GSH-Px的活性;运动可降低大鼠肝脏GSH-Px的活性;染毒后运动可减少肝脏MDA的含量,升高肝脏SOD、CAT和GSH-Px的活性。研究表明,TCDD急性暴露可导致大鼠肝细胞功能受损,导致大鼠肝脏发生氧化应激。8周有氧运动改善TCDD急性暴露诱导的肝细胞损伤,改善肝脏氧化应激,这可能是运动改善TCDD肝毒性的机制之一。

关键词(KeyWords)： 2,3,7,8-四氯二苯并二恶英;大鼠;肝脏抗氧化能力;运动

Abstract:

Keywords:

基金项目(Foundation): 北京体育大学自主科研课题(2014YB015);; 全国重点地区环境与健康专项调查(21111011101EHH(2011)-505)

作者(Author): 张弛;闫会萍;陆一帆;翁凯翔;武铁男;胡兵;王娟娟;刘一凝;魏东洋;许振成;

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参考文献(References)：

• [1]Malisch R,Kotz A.Dioxins and PCBs in feed and foodreview from European perspective[J].Science of the Total Environment,2014,491-492:2-10
• [2]Sorg O.Ah R signalling and dioxin toxicity[J].Toxicology Letters,2014,230(2):225-233
• [3]Diliberto J J,Jackson J A,Birnbaum L S.Comparison of2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)disposition following pulmonary,oral,dermal,and parenteral exposures to rats[J].Toxicology and Applied Pharmacology,1996,138(1):158-68
• [4]Ciftci O,Vardi N,Ozdemir I.Effects of quercetin and chrysin on 2,3,7,8-tetrachlorodibenzo-p-dioxin induced hepatotoxicity in rats[J].Environmental Toxicology,2013,28(3):146-154
• [5]Turkez H,Geyikoglu F,Yousef M I.Beneficial effect of astaxanthin on 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced liver injury in rats[J].Toxicology&Industrial Health,2013,29(7):591-599
• [6]李军汉,苏全生,孙君志,等.内质网应激介导细胞凋亡在运动预防非酒精性脂肪肝形成中的作用[J].北京体育大学学报,2015,10:8Li J H,Su Q S,Sun J Z,et al.Effects of exercise on endoplasmic reticulum stress induced apoptosis of liver cells in rats with non-alcoholic fatty liver diseases model[J].Journal of Beijing Sport University,2015,10:8(in Chinese)
• [7]晋娜,陈文鹤.中小强度有氧运动对肥胖症患者脂肪肝的影响[J].上海体育学院学报,2013,36(6):58-61Jin N,Chen W H.The influence of aerobic exercise with moderate and low intensity on fatty liver of obese patients[J].Journal of Shanghai University of Sport,2013,36(6):58-61(in Chinese)
• [8]张裕中,王银晖,陈晓光,等.游泳运动和白藜芦醇对Ⅱ型糖尿病大鼠肝糖原及肝脏GLUT2的影响[J].中国运动医学杂志,2014,33(2):135-140Zhang Y Z,Wang Y H,Chen X G,et al.Effects of swimming and resveratrol adminnstrarion on hepatic glycogen and GLU2 in the liver of type 2 diabetic rat[J].Chinese Journal of Sports Medicine,2014,33(2):135-140(in Chinese)
• [9]Zsolt R,Zhongfu Z,Erika K,et al.Oxygen consumption and usage during physical exercise:The balance betw een oxidative stress and ROS-dependent adaptive signaling[J].Antioxidants&Redox Signaling,2013,18(10):1208-1246
• [10]蔡爱芳.运动对二恶英致大鼠脂质合成代谢障碍的干预作用及其机制研究[D].北京:北京体育大学,2013Cai A F.Intervention effect and mechanism of exercise on dioxin caused lipid anabolism disorders in rats[D].Beijing:Beijing Sport University,2013(in Chinese)
• [11]Kalaiselvan I,Samuthirapandi M,Govindaraju A,et al.Olive oil and its phenolic compounds(hydroxytyrosol and tyrosol)ameliorated TCDD-induced heptotoxicity in rats via inhibition of oxidative stress and apoptosis[J].Pharmaceutical Biology,2016,54(2):1-9
• [12]Lu H,Cui W,Klaassen C D.Nrf2 protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)-induced oxidative injury and steatohepatitis[J].Toxicology and Applied Pharmacology,2011,256(2):122-135
• [13]Sorg O.Ah R signalling and dioxin toxicity[J].Toxicology Letters,2014,230:225-233
• [14]Watson J D,Prokopec S D,Smith A B,et al.TCDD dysregulation of 13 AHR-target genes in rat liver[J].Toxicology and Applied Pharmacology,2014,274(3):445-454
• [15]Pierre S,Cheval ier A,Teixeira-Clerc F,et al.Aryl hydrocarbon receptor-dependent induction of liver fibrosis by dioxin[J].Toxicological Sciences,2014,137(1):114-124
• [16]Türkez H,Geyikoglu F,Yousef M I.Ameliorative effect of docosahexaenoic acid on 2,3,7,8-tetrachlorodibenzo-pdioxin-induced histological changes,oxidative stress,and DNA damage in rat liver[J].Toxicology&Industrial Health,2012,28(8):687-696
• [17]Aly H A,El-Shitany N A,El-Beshbishy H A,et al.Ameliorative effect of lycopene against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced rat liver microsomal toxicity:An in vitro study[J].Toxicology&Industrial Health,2015,31(10):938-950
• [18]赵丽,熊开宇,王正珍,等.运动与毒物兴奋效应[J].中国老年学杂志,2011,31(11):2134-2136Zhao L,Xiong K Y,Wang Z Z,et al.Exercise and hormesis[J].Chinese Journal of Gerontology,2011,31(11):2134-2136(in Chinese)
• [19]Vezzoli A,Pugliese L,Marzorati M,et al.Time-course changes of oxidative stress response to high-intensity discontinuous training versus moderate-intensity continuous training in masters runners[J].Plo S one,2014,9(1):e87506
• [20]Sugama K,Suzuki K,Yoshitani K,et al.Changes of thioredoxin,oxidative stress markers,inflammation and muscle/renal damage follow ing intensive endurance exercise[J].Exercise Immunology Review,2015,21:130-142
• [21]Barcelos R P,Souza M A,Amaral G P,et al.Caffeine supplementation modulates oxidative stress markers in the liver of trained rats[J].Life Sciences,2014,96(1-2):40-45
• [22]Hu X,Duan Z,Hu H,et al.Proteomic profile of carbonylated proteins in rat liver:Exercise attenuated oxidative stress may be involved in fatty liver improvement[J].Proteomics,2013,13(10-11):1755-1764
• [23]Kwon T D,Lee M W,Kim K H.The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat[J].Journal of Exercise Nutrition&Biochemistry,2014,18(1):9-17