运动对2,3,7,8-四氯二苯并二恶英急性暴露大鼠肝脏抗氧化能力的影响Effect of Exercise on the Activity of Liver Antioxidant Ability in 2,3,7,8-Tetrachlorodibenzo-p-dioxin( TCDD)-Exposed Rats
张弛;闫会萍;陆一帆;翁凯翔;武铁男;胡兵;王娟娟;刘一凝;魏东洋;许振成;
摘要(Abstract):
观察8周中等强度游泳运动对2,3,7,8-四氯二苯并二恶英(2,3,7,8-tetrachlorodibenzo-p-dioxin,2,3,7,8-TCDD)急性暴露大鼠肝脏氧化应激的影响。以8周龄雄性Sprague Dawley大鼠为研究对象,将大鼠随机分为玉米油静养组(NC组)、玉米油运动组(EC组)、TCDD静养组(NT)和TCDD运动组(ET组)。将TCDD溶于玉米油中,NT和ET组大鼠按照10μg·kg-1(以单位体重计)腹腔注射TCDD,NC和EC组大鼠注射等量玉米油。正式实验开始后,EC和ET组大鼠进行运动(尾部负重5%游泳30min),每周运动5 d,共8周,NC和NT组大鼠不进行任何运动干预。8周后,称重并宰杀大鼠,收集血清和肝组织样本,待测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的活性;肝组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)以及谷胱甘肽过氧化物酶(GSH-Px)的活性。将数据进行多因素方差分析,结果表明,染毒可升高大鼠血清AST的活性,增加肝脏MDA的含量,降低肝脏SOD、CAT和GSH-Px的活性;运动可降低大鼠肝脏GSH-Px的活性;染毒后运动可减少肝脏MDA的含量,升高肝脏SOD、CAT和GSH-Px的活性。研究表明,TCDD急性暴露可导致大鼠肝细胞功能受损,导致大鼠肝脏发生氧化应激。8周有氧运动改善TCDD急性暴露诱导的肝细胞损伤,改善肝脏氧化应激,这可能是运动改善TCDD肝毒性的机制之一。
关键词(KeyWords): 2,3,7,8-四氯二苯并二恶英;大鼠;肝脏抗氧化能力;运动
基金项目(Foundation): 北京体育大学自主科研课题(2014YB015);; 全国重点地区环境与健康专项调查(21111011101EHH(2011)-505)
作者(Author): 张弛;闫会萍;陆一帆;翁凯翔;武铁男;胡兵;王娟娟;刘一凝;魏东洋;许振成;
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