酶催化降解四溴双酚A反应引起的碳纳米管急性毒性变化The Toxic Effect of Multiwall Carbon Nanotubes Modified by the Peroxidase-Mediated Tetrabromobisphenol A Reaction on Daphnia magna and Scenedesmus obliquus
卢坤;董仕鹏;高士祥;毛亮;
摘要(Abstract):
辣根过氧化物酶催化去除四溴双酚A的反应能够改变多壁碳纳米管(multiwall carbon nanotubes,MWCNTs)在水环境中的稳定性。为探究该反应对碳纳米管生态毒性效应的影响,选择大型蚤(Daphnia magna)和斜生栅藻(Scenedesmus obliquus)作为受试生物,分别考察了反应前后MWCNTs对大型蚤的急性毒性以及对斜生栅藻的生长抑制和藻细胞色素的影响。结果表明,在1~10 mg·L-1的暴露浓度下,与原始的MWCNTs相比,反应后的MWCNTs对大型蚤的急性毒性明显减弱。在10 mg·L-1的暴露剂量下,原始的MWCNTs造成大型蚤的死亡率高达65%,而反应后的MWCNTs引起大型蚤的死亡率仅为23%。此外,反应前后的MWCNTs对斜生栅藻的生长均有抑制作用。随着暴露浓度的增大,藻细胞密度、叶绿素a和b的含量逐渐降低。与原始MWCNTs相比,反应后的MWCNTs对斜生栅藻的毒性明显减弱。上述研究结果为评价MWCNTs的环境风险提供了基础数据。
关键词(KeyWords): 碳纳米管;四溴双酚A;大型蚤;斜生栅藻;毒性;环境风险
基金项目(Foundation): 江苏省自然科学基金(BK2011576)
作者(Author): 卢坤;董仕鹏;高士祥;毛亮;
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DOI:
参考文献(References):
- [1]Popov V.Carbon nanotubes:Properties and application[J].Materials Science and Engineering:Reports,2004,43(3):61-102
- [2]Qu X L,Alvarez P J,Li Q L.Applications of nanotechnology in water and wastewater treatment[J].Water Research,2013,47(12):3931-3946
- [3]De Volder M F,Tawfick S H,Baughman R H,et al.Carbon nanotubes:Present and future commercial applications[J].Science,2013,339(6119):535-539
- [4]Petersen E J,Zhang L W,Mattison N T,et al.Potential release pathways,environmental fate,and ecological risks of carbon nanotubes[J].Environmental Science&Technology,2011,45(23):9837-9856
- [5]Schlagenhauf L,Buerki-Thurnherr T,Kuo Y Y,et al.Carbon nanotubes released from an epoxy-based nanocomposite:Quantification and particle toxicity[J].Environmental Science&Technology,2015,49(17):10616-10623
- [6]Boncel S,Kyziol-Komosinska J,Krzyzewska I,et al.Interactions of carbon nanotubes with aqueous/aquatic media containing organic/inorganic contaminants and selected organisms of aquatic ecosystems-A review[J].Chemosphere,2015,136:211-221
- [7]Su Y,Yan X M,Pu Y B,et al.Risks of single-walled carbon nanotubes acting as contaminants-carriers:Potential release of phenanthrene in Japanese medaka(Oryzias latipes)[J].Environmental Science&Technology,2013,47(9):4704-4710
- [8]Chen W,Zhu D Q,Zheng S,et al.Catalytic effects of functionalized carbon nanotubes on dehydrochlorination of 1,1,2,2-tetrachloroethane[J].Environmental Science&Technology,2014,48(7):3856-3863
- [9]Fu H Y,Guo Y,Chen W,et al.Reductive dechlorination of hexachloroethane by sulfide in aqueous solutions mediated by graphene oxide and carbon nanotubes[J].Carbon,2014,72:74-81
- [10]Liu Y,Zhao Y L,Sun B Y,et al.Understanding the toxicity of carbon nanotubes[J].Accounts of Chemical Research,2012,46(3):702-713
- [11]Hu X G,Zhou M,Zhou Q X.Ambient water and visible-light irradiation drive changes in graphene morphology,structure,surface chemistry,aggregation,and toxicity[J].Environmental Science&Technology,2015,49(6):3410-3418
- [12]Mu L,Gao Y,Hu X G.Characterization of biological secretions binding to graphene oxide in water and the specific toxicological mechanisms[J].Environmental Science&Technology,2016,50(16):8530-8537
- [13]Vamos-Vigyazo L.Polyphenol oxidase and peroxidase in fruits and vegetables[J].Critcal Review in Food Science Nutrition,1981,15(1):49-127
- [14]Tien M,Kirk T K.Lignin peroxidase of phanerochaete chrysosporium[J].Methods in Enzymology,1988,161:238-249
- [15]Huang Q G,Webe W J.Transformation and removal of bisphenol A from aqueous phase via peroxidase-mediated oxidative coupling reactions:Efficacy,products,andpathways[J].Environmental Science&Technology,2005,39(16):6029-6036
- [16]Lu K,Huang Q G,Wang P,et al.Physicochemical changes of few-layer graphene in peroxidase-catalyzed reactions:Characterization and potential ecological effects[J].Environmental Science&Technology,2015,49(14):8558-8565
- [17]Li J H,Peng J B,Zhang Y,et al.Removal of triclosan via peroxidases-mediated reactions in water:Reaction kinetics,products and detoxification[J].Journal of Hazardous Materials,2016,310:152-160
- [18]Colosi L M,Pinto R A,Huang Q G,et al.Peroxidasemediated degradation of perfluorooctanoic acid[J].Environmental Toxicology and Chemistry,2009,28(2):264-271
- [19]De Wit C A.An overview of brominated flame retardants in the environment[J].Chemosphere,2002,46(5):583-624
- [20]Feng Y P,Colosi L M,Gao S X,et al.Transformation and removal of tetrabromobisphenol A from water in the presence of natural organic matter via laccase-catalyzed reactions:Reaction rates,products,and pathways[J].Environmental Science&Technology,2013,47(2):1001-1008
- [21]Lu K,Huang Q G,Xia T,et al.The potential ecological risk of multiwall carbon nanotubes was modified by the radicals resulted from peroxidase-mediated tetrabromobisphenol A reactions[J].Environmental Pollution,2017,220:263-273
- [22]Chen K L,Elimelech M.Relating colloidal stability of fullerene(C60)nanoparticles to nanoparticle charge and electrokinetic properties[J].Environmental Science&Technology,2009,43(19):7270-7276
- [23]Mitchell B G,Kiefer D A.Determination of Absorption and Fluoresence Excitation Spectra for Phytoplankton[M].Berlin:Springer Berlin Heidelberg,1984,8:157-169
- [24]Zhu X,Zhu L,Chen Y S,et al.Acute toxicities of six manufactured nanomaterial suspensions to Daphnia magna[J].Journal of Nanoparticle Research,2009,11(1):67-75
- [25]Petersen E J,Pinto R A,Mai D J,et al.Influence of polyethyleneimine graftings of multi-walled carbon nanotubes on their accumulation and elimination by and toxicity to Daphnia magna[J].Environmental Science&Technology,2011,45(3):1133-1138
- [26]杨晓静,陈灏,闫海,等.纳米二氧化钛和单壁碳纳米管对普通小球藻生长的抑制效应[J].生态毒理学报,2010,5(1):38-43Yang X,Chen H,Yan H,et al.Effects of nano-Ti O2and single-walled carbon nanotubes on the growth of Chlorella vulgaris[J].Asian Journal of Ecotoxicology,2010,5(1):38-43(in Chinese)
- [27]王莉,唐丽雅,魏晨曦,等.邻苯二甲酸二(2-乙基)己酯对斜生栅藻的生态毒性作用[J].生态毒理学报,2009,3(4):452-456Wang L,Tang L Y,Wei C X,et al.Ecological toxicity of di-(2-ethylhexyl)phthalate on Scenedesmus obliquus[J].Asian Journal of Ecotoxicology,2009,3(4):452-456(in Chinese)
- [28]Geoffroy L,Dewez D,Vernt G,et al.Different physiological parameters used in evaluation of oxyfluorfen effect on S.obliquus:Validity of parameters as biomarkers[J].Archives of Environmental Contamination and Toxicology,2003,45(4):439-454