3种氯酚类化合物对河蚬的毒性和氧化应激Toxicity and Oxidative Stress of Three Chlorophenols to Freshwater Clam Corbicula fluminea
金小伟;查金苗;许宜平;王子健;
摘要(Abstract):
以河蚬(Corbicula fluminea)为试验生物,研究了2,4-DCP、2,4,6-TCP和PCP的急性毒性和21d慢性毒性,并在同一条件下测试了这3种氯酚类化合物对河蚬软体组织抗氧化系统的影响.结果显示:2,4-DCP、2,4,6-TCP和PCP对河蚬96hLC50值分别为19.25(17.58~21.20)、41.98(35.48~58.24)和0.23(0.18~0.27)mg·L-1;以致死作为观察指标,其21d无观察效应浓度(NOEC)和最低观察效应浓度(LOEC)值分别为1.00、2.00、0.06mg·L-1和2.00、4.00、0.08mg·L-1.3种氯酚类化合物对河蚬软体组织抗氧化系统几种酶的活性均表现出了不同程度的影响,其中对SOD和GSH-Px的影响最为明显.2,4,6-TCP和PCP对SOD均表现为抑制作用,对GSH-Px表现为先诱导后抑制;2,4-DCP对SOD和GSH-Px表现为明显的诱导作用;3种氯酚类化合物对CAT均表现为明显的诱导作用;对GST活性也有一定影响,但与对照组相比变化范围不大(62.95%~137.04%).3种氯酚类化合物暴露后生物体抗氧化酶有不同的响应,表明不同氯酚类化合物所导致的氧化损伤及其应激机制不同.
关键词(KeyWords): 氯酚类化合物;河蚬;毒性;抗氧化酶活性
基金项目(Foundation): 环保公益性行业科研专项(No.200709034);; 国家自然科学基金(No.40703025);; 国家“863”计划资助项目(No.2007AA06A405);; 环境模拟与污染控制国家重点联合实验室导向课题(No.S133)
作者(Author): 金小伟;查金苗;许宜平;王子健;
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参考文献(References):
- Aldrigde D C,Muller S J.2001.The asiatic clam,Corbiculafluminea,in Britain:Current status and potential impacts[J].Journal of Conchology,37(2):177-182
- Bradford M M.1976.A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding[J].Analytical Biochemistry,72:248-254
- Chen C Y,Lin K C.1997.Optimization and performanceevaluation of the continuous algal toxicity test[J].EnvironmentalToxicology and Chemistry,16:1337-1344
- Czaplicka M.2004.Sources and transformations of chlorophenolsin the natural environment[J].Science of the Total Environment,322(1-3):21-39
- Daniel V,Huber W,Bauer K,Opelz G.1995.Impaired in-vitrolymphocyte responses in patients with elevated pentachlorophenol(PCP)blood levels[J].Archives of Environmental Health,50(4):287-292
- Drevet J R.2006.The antioxidant glutathione peroxidase famlilyand spermatozoa:A complex story[J].Molecular and CellularEndocrinology,250(1-2):70-79
- Elstner E F,Heupel A.1976.Inhibition of nitrite formation fromhydroxylam-moniumchloride:a simple assay for superoxidedismutase[J].Analytical Biochemistry,70:616-620
- Gao J,Liu L,LiuX,Zhou H,Huang S,Wang Z.2008.Levelsand spatial distribution of chlorophenols2,4-dichlorophenol,2,4,6-trichlorophenol,and pentachlorophenol in surface water ofChina[J].Chemosphere,71(6):1181-1187
- Gao X J,Fang Y J,Wang C H,Gao Z X,Yu S Y,Ou G R.2009.Toxicity effects of two kinds of typical chlorophenols onhepatocytes of Gobiocypris rarus[J].Asian Journal of Ecotoxicology,4(5):682-687(in Chinese)
- Habig W H,Pabst M J,Jakoby W B.1974.Glutathione S-transferases.The first enzymatic step in mercapturic acid formation[J].Journal of Biological Chemistry,249(22):7130-7139
- Hafeman D G,Sunde R A,Hoekstra W G.1973.Effect ofdietary selenium and erythrocyte and liver glutathione peroxidasein the rat[J].Journal of Nitrition,104:580-587
- Lei B L,Jin X W,Huang S B,Wang Z J.2009.Discussion ofquality criteria for three chlorophenols in Taihu Lake[J].AsianJournal of Ecotoxicology,4(1):40-49(in Chinese)
- Li T Y,Huang S B,Sun F,Zha J M,Qiao M,Wang Z J.2008.Bioaccumulation by Corbicula fluminea of polycyclicaromatic hydrocarbons from sediments in Meiliang Bay,TaihuLake[J].Acta Scientiae Circumstantiae,28(11):2354-2360(inChinese)
- Liu M,Xiong B X.2008.Ecological characteristics of Corbiculafluminea and its effect on the heavy metals accumulation[J].Journal of Anhui Agricultural Sciences,36(1):221-224(inChinese)
- Mather-Mihaich E,Di Giulio R T.1991.Oxidant,mixedfunction oxidase and peroxisomal responses in channel catfishexposed to a bleached kraft mill effluent[J].Archives ofEnvironmental Contamination and Toxicology,20(3):391-397
- Ministry of Environmental Protection of the People’s Republic ofChina.1986.Technical Criteria for Biological Monitoring(WaterEnvironment)[R].Beijing:China Environmental Science Press,95(in Chinese)
- Ministry of Health of the People’s Republic of China,Standardization Administration of the People’s Republic of China.2007.GB5749-2006Standards for Drinking Water Quality[S].Beijing:Standards Press of China(in Chinese)
- Petushok N,Gabryelak T,Pa覥ecz D,Zavodnik L,Szollosi VargaI,Deér K A.2002.Comparative study of the xenobioticmetabolising system in the digestive gland of the bivalvemolluscs in different aquatic ecosystems and in aquariaexperiments[J].Aquatic Toxicology,61(1-2):65-72
- Rodriguez-Ariza A.1993.Biochemical indicators of oxidativestress in fish from polluted littoral areas[J].Canadian Journal ofFisheries and Aquatic Sciences,50:2568-2573
- Shi Y J,LüY L,Ren H C,Liang D.2003.Recent progress insecientific research on persistent organic pollutants(POPs)[J].World Sci-Tech R&D,25(2):73-78(in Chinese)
- Tugiyono,Gagnon M M.2002.Metabolic disturbances in fishexposed to sodium pentachlorophenate(NaPCP)and3,3′,4,4′,5-pentachlosobiphenyl(PCB126),individually or combined[J].Comparative Biochemistry and Physiology,Part C,132(4):425-435
- U.S.EPA.1985.Guidelines for Deriving Numerical NationalWater Quality Criteria for the Protection of Aquatic Organismsand Their Uses[R].National Technical Information ServiceAccession Number PB85-227049.Washington,D C:U.S.Environmental Protection Agency
- Vidal M L,Basseres A,Narbonne J F.2001.Interest of amultibiomarker approach in the assessment of freshwaterecosystem quality:laboratory and field studies[J].Water Scienceand Technology,44(223):305-312
- Winston G W,Di Guilio R J.1991.Prooxidant and antioxidantmechanisms in aquatic organisms[J].Aquatic Toxicology,19:137-161
- Xia Q,Chen Y Q,Liu X B.2004.Criteria and Standards forDrinking Water Quality[M].Beijing:Standards Press of China(in Chinese)
- Yin D Q,Hu S Q,Jin H J,Yu L W.2003a.Derivingfreshwater quality criteria for2,4,6-trichlorophenol for protectionof aquatic life in China[J].Chemosphere,52(1):67-73
- Yin D Q,Jin H J,Yu L W,Hu S Q.2003b.Derivingfreshwater quality criteria for2,4-dichlorophenol for protection ofaquatic life in China[J].Environmental Pollution,122(2):217-222
- Zeng F,Wang X G,Li F B,Li X F,Liu L,Gu M H.2007.Reductive transformation of pentachlorophenol in the interface ofpaddy soil colloids and water[J].Asian Journal of Ecotoxicology,2(1):49-56(in Chinese)
- Zha J M,Wang Z J,Schlenk D.2006.Effects of pentachlorophenolon the reproduction of Japanese medaka(Oryzias latipes)[J].Chemico-Biological Interactions,161(1):26-36
- Zhang J F,Shen H,Wang X,Wu J,Xue Y.2004.Effects ofchronic exposure of2,4-dichlorophenol on the antioxidant systemin liver of freshwater fish Carassius auratus[J].Chemosphere,55(2):167-174
- Zhang J F,Wang X R.2003.Effects of long-term exposure oflow-level2,4-dechlorophenol on the antioxidant defense systemin liver of Carassius auratus[J].Environmental Science,24(5):136-140(in Chinese)
- Zhang X Y,Zha J M,Li W,Yang L,Wang Z J.2008.Effects of2,4-dichlorophenol on the expression of vitellogeninand estrogen receptor genes and physiology impairments inChinese rare minnow(Gobiocypris rarus)[J].EnvironmentalToxicology,23(6):694-701
- 高先军,房彦军,王彩红,高志贤,俞诗源,欧国荣.2009.两种典型氯酚类化合物暴露致稀有鮈鲫肝细胞的损伤[J].生态毒理学报,4(5):682-687
- 国家环境保护局.1986.生物监测技术规范(水环境部分)[R].北京:中国环境科学出版社,95
- 雷炳莉,金小伟,黄圣彪,王子健.2009.太湖流域3种氯酚类化合物水质基准的探讨[J].生态毒理学报,4(1):40-49
- 李天云,黄圣彪,孙凡,查金苗,乔敏,王子健.2008.河蚬对太湖梅梁湾沉积物多环芳烃的生物富集[J].环境科学学报,28(11):2354-2360
- 刘敏,熊邦喜.2008.河蚬的生态习性及其对重金属的富集作用[J].安徽农业科学,36(1):221-224
- 史雅娟,吕永龙,任鸿昌,梁丹.2003.持久性有机污染物研究国际发展动态[J].世界科技研究与发展,25(2):73-78
- 夏青,陈艳卿,刘宪兵.2004.水质基准与水质标准[M].北京:中国标准出版社
- 曾芳,王旭刚,李芳柏,黎晓峰,刘亮,顾明华.2007.2种水稻土胶体界面五氯酚的还原脱氯转化[J].生态毒理学报,2(1):49-56
- 张景飞,王晓蓉.2003.2,4-二氯苯酚低浓度长期暴露对鲫鱼肝脏抗氧化系统的影响[J].环境科学,24(5):136-140
- 中华人民共和国卫生部,国家标准化管理委员会.2007.GB5749-2006生活饮用水卫生标准[S].北京:中国标准出版社