全氟辛烷磺酸对哮喘小鼠炎症反应的影响Effects of Perfluooctane Sulfonate on Inflammatory Responses in Asthmatic Mice
曹文锐;任静;贺妮楠;陈静;秦小迪;董光辉;曾晓雯;
摘要(Abstract):
探讨全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)暴露对哮喘小鼠Th1/Th2炎症反应的影响。选择36只雄性C57BL/6J小鼠,随机分为6组:对照组、卵清蛋白(ovalbumin,OVA)单独致敏组、PFOS染毒组(0.1、1、5、10 mg·kg~(-1)·d~(-1) PFOS+OVA致敏),每组6只。染毒和诱发哮喘后第26天收集肺泡灌洗液(BALF)进行有核细胞总数和嗜酸性粒细胞计数;用Luminex技术测定BALF中细胞因子白细胞介素-5(interleukin-5,IL-5)、白细胞介素-4(interleukin-4,IL-4)和γ干扰素(interferon-γ,IFN-γ)水平;肺组织切片染色观察其病理变化。随PFOS暴露浓度的增加,嗜酸粒细胞总数呈上升趋势(P<0.05),IL-5/IFN-γ比值增加(P<0.05);其中10 mg·kg~(-1)·d-1PFOS+OVA组BALF中的白细胞总数显著高于OVA单独组(P<0.05),IL-4水平及IL-4/IFN-γ比值显著高于OVA单独组(P<0.05),且与单独OVA组相比小鼠气道粘膜及粘膜下层厚度更厚,炎症细胞浸润更明显,PAS染色阳性的气道上皮细胞明显更高。PFOS能加重哮喘小鼠肺部的炎症反应并诱导Th2型细胞因子的表达。
关键词(KeyWords): 全氟辛烷磺酸;哮喘小鼠;炎症反应;哮喘;Th1/Th2型细胞因子
基金项目(Foundation): 中山大学青年教师重点培育项目(17ykzd14);中山大学实验室开放基金项目(20160225);; 国家自然科学基金项目(81472936,81673127);; 有机地球化学国家重点实验室开放基金(NO.SKLOG-201608)
作者(Author): 曹文锐;任静;贺妮楠;陈静;秦小迪;董光辉;曾晓雯;
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