磁铁矿纳米颗粒与Pb~(2+)复合物对大鼠肾细胞的毒性研究The Cytotoxic Effects of Magnetite Nanoparticle-Pb~(2+) Complex on Rat Kidney Cells
陈力可;郭昌胜;刘淼;李专;徐建;
摘要(Abstract):
磁铁矿纳米颗粒(magnetite nanoparticles, MNPs)是一种环境友好型吸附剂,广泛应用于废水中Pb~(2+)的处理。目前,有不少关于MNPs毒性的研究,但对MNPs处理Pb~(2+)形成的复合物的毒性却鲜有报道,其复合毒性亟待深入研究。本文以大鼠肾细胞(NRK)作为细胞模型,系统研究不同Pb含量的MNPs-Pb复合物,以及相应浓度的MNPs和Pb~(2+),对大鼠肾细胞活性、细胞形态和密度的影响,考察细胞对纳米颗粒的摄取以及细胞凋亡的作用机制,评估MNPs-Pb的毒性效应。结果表明,在本实验浓度和暴露时间(12 h)条件下,Pb~(2+)能够显著抑制细胞活性,改变细胞形态,促进细胞凋亡,对细胞有显著的毒性效应,并且呈现剂量相关性;利用MNPs吸附水环境中Pb~(2+)形成的复合物MNPs-Pb对大鼠肾细胞没有显著的毒性作用(P<0.05),MNPs对Pb~(2+)的吸附可能是Pb~(2+)的细胞毒性降低的原因。
关键词(KeyWords): MNPs;Pb~(2+);复合物;大鼠肾细胞;细胞毒性;细胞凋亡;复合污染
基金项目(Foundation): 国家自然科学基金资助项目(51208482)
作者(Author): 陈力可;郭昌胜;刘淼;李专;徐建;
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DOI:
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