李峰;石辉;

• 1:西安建筑科技大学环境与市政工程学院

摘要(Abstract)：

为了探讨一次性递增负荷运动及不同浓度细颗粒物(PM2.5)暴露对大鼠己糖激酶(hexokinase,HK)-丙酮酸激酶(pyruvatekinase,PK)及异柠檬酸脱氢酶(isocitratedehydrogenase,IDH)活性影响的机制,将30只雄性Wistar SPF(Specific Pathogen Free,SPF)大鼠随机分为安静(QC)、运动对照组(EC)、低剂量PM2.5+运动组(LPE)、中剂量PM2.5+运动组(MPE)、高剂量PM2.5+运动组(HPE),采用一次性气管滴注染毒后进行递增负荷跑台训练,并通过酶联免疫法(enzyme-linked immunosorbent assay,ELISA)测定大鼠血清及肝脏组织HK,PK,IDH活性。结果表明,与安静组相比,运动对照组HK,PK酶活性下降,IDH活性升高,差异有统计学意义(p<0.01)。和EC组相比,LPE,MPE,HPE组各组织中HK,PK,IDH活性均下降,差异有统计学意义(p<0.05或p<0.01)。实验表明,运动可以降低大鼠的HK,PK活性而增强IDH活性;随着浓度的增加,HK,PK,IDH活性与PM2.5暴露剂量具有相关性降低。

关键词(KeyWords)： 细颗粒物;糖代谢;限速酶;糖酵解

Abstract:

Keywords:

基金项目(Foundation): 青年科技基金(No.QN1043)

作者(Author): 李峰;石辉;

Email:

DOI:

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