黄益宗;隋立华;王玮;耿春梅;殷宝辉;

• 1:中国科学院生态环境研究中心
• 2:中国石油化工股份有限公司青岛安全工程研究院
• 3:中国环境科学研究院

摘要(Abstract)：

臭氧(O3)被认为是重要的气污染物之一,水稻又是主要的粮食作物,因而准确地评估O3浓度升高对水稻生长发育的影响具有十分重要的意义。采用开顶式气室法模拟研究了O3对水稻叶片可见伤害症状、氮代谢、脯氨酸和谷胱甘肽含量的影响。结果显示,O3污染胁迫会导致水稻叶片产生明显的伤害症状,具体表现为:老叶叶鞘褪绿,有褐斑,直至完全干枯;稻穗小且黄化,籽粒不饱满;水稻成熟期提前等。O3浓度升高对水稻叶片的硝酸还原酶活性有显著影响。当O3浓度为40、80和120nL.L-1时,水稻叶片硝酸还原酶活性与对照组相比均降低,其中,分蘖期分别降低了25.3%、67.4%和86.3%;拔节期分别降低了57.4%、75.7%和97.8%;抽穗期分别降低了91.0%、97.2%和99.3%;乳熟期分别降低了89.5%、89.5%和96.7%。水稻叶片铵态氮和硝态氮含量随着O3浓度的升高而显著地降低,例如当O3浓度为40、80和120nL.L-1时,与对照相比,水稻叶片硝态氮含量分别降低46.3%、52.7%和65.7%,铵态氮含量分别降低6.5%、12.9%和43.4%。O3污染胁迫下水稻叶片脯氨酸含量在不同生长期变化不同,分蘖期、拔节期和抽穗期脯氨酸含量在40nL.L-1浓度O3熏蒸下急剧地提高,但是随着O3浓度的增加,脯氨酸含量又不断地降低。在水稻乳熟期,脯氨酸含量均随着O3浓度的增加而显著地下降。O3污染胁迫导致水稻叶片还原型谷胱甘肽(GSH)含量显著低于对照组,而氧化型谷胱甘肽(GSSG)含量显著高于对照组。当O3浓度为40、80和120nL.L-1时,乳熟期水稻叶片GSH含量分别比对照组降低68.7%、80.2%和78.2%,GSSG含量分别比对照提高494.4%、527.2%和439.8%。研究表明,O3污染胁迫对水稻叶片氮代谢和抗氧化系统产生了极显著的影响。

关键词(KeyWords)： 臭氧;水稻;氮代谢;硝酸还原酶;抗氧化系统;谷胱甘肽

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(41071336);; 国家环保公益性行业科研专项(200809152)

作者(Author): 黄益宗;隋立华;王玮;耿春梅;殷宝辉;

Email:

参考文献(References)：

• [1]Vingarzan R.A review of surface ozone background levels and trends[J].Atmospheric Environment,2004,38(21):3431-3442
• [2]Selin N E,Wu S,Nam K M,et al.Global health and eco-nomic impacts of future ozone pollution[J].Environmen-tal Research Letters,2009,4(4):1-9
• [3]金明红.大气O3浓度变化对农作物影响的实验研究[D].北京:中国科学院研究生院,2001:1-107Jin M H.Experimental study on the impacts of O3con-centration change on crops[D].Beijing:Graduate School of the Chinese Academy of Sciences,2001:1-107(in Chinese)
• [4]白建辉,王明星,Graham J,等.鼎湖山臭氧、氮氧化物和太阳可见光辐射相互关系的研究[J].环境科学学报,2000,20(2):173-178Bai J H,Wang M X,Graham J,et al.The study on the relation of surface ozone,NO Xand solar visible radiation in Dinghushan station[J].Acta Scientiae Circumstantiae,2000,20(2):173-178(in Chinese)
• [5]张文方.北京城区臭氧浓度变化规律及预测方法研究[D].北京:北京林业大学,2010:30-45Zhang W F.Study on the variation and predietion method of ozone concentration in Beijing urban area[D].Beijing:Beijing Forestry University,2010:30-45(in Chinese)
• [6]Shao M,Tang X,Zhang Y,et al.City clusters in China:Air and surface water pollution[J].Frontiers in Ecology and the Environment,2006,4(7):353-361
• [7]Cho K,Tiwari S,Agrawal S B,et al.Tropospheric ozone and plants:Absorption,responses,and consequences[J].Reviews of Environmental Contamination&Toxicology,2011,212:61-111
• [8]Adams R M,Glyer J,Mccarl B.The NCLAN Economic Assessment:Approach,Findings and Implications[M].London,UK:Elsevier,Assessment of Crop Loss fromAir Pollutants,1988:473-504
• [9]Holland M,Mills G,Hayes F.Economic Assessment of Crop Yield Losses from Ozone Exposure.Part of the UNECE International Cooperative Programme on Vegeta-tion[R].Progress Report(April2001～March2002)Contract EPG,2002,1(3):170
• [10]Feng Z,Jin M,Zhang F Z,et al.Effects of ground-level ozone(O3)pollution on the yields of rice and winter wheat in the Yangtze River Delta[J].Journal of Environ-mental Sciences,2003,15(3):360-362
• [11]白月明,郭建平,王春乙,等.水稻与冬小麦对臭氧的反应及敏感性实验研究[J].中国生态农业学报,2002,10(1):13-16Bai Y M,Guo J P,Wang C Y,et al.The reaction and sensitivity experiment of O3on rice and winter wheat[J].Chinese Journal of Eco-Agriculture,2002,10(1):13-16(in Chinese)
• [12]金明红,黄益宗.臭氧污染胁迫对农作物生长与产量的影响[J].生态环境,2003,12(4):482-486Jin M H,Huang Y Z.Review of crops damaged and yield loss by ozone stress[J].Ecology and Environment,2003,12(4):482-486(in Chinese)
• [13]姚芳芳,王效科,陈展,等.农田冬小麦生长和产量对臭氧动态暴露的响应[J].植物生态学报,2008,32(1):212-219Yao F F,Wang X K,Chen Z,et al.Response of photo-synthesis,growth and yield of field-grown winter wheat to ozone exposure[J].Acta Phytoecologica Sinica,2008,32(1):212-219(in Chinese)
• [14]金东艳,赵天宏,付宇,等.臭氧浓度升高对大豆光合作用及产量的影响[J].大豆科学,2009,28(4):632-635Jin D Y,Zhao T H,Fu Y,et al.Effects of elevated ozone concentration on soybean photosynthesis and yield[J].Soybean Science,2009,28(4):632-635(in Chinese)
• [15]石春红,郑有飞,吴芳芳,等.大气中臭氧浓度增加对根际和非根际土壤微生物的影响[J].土壤学报,2009,46(5):894-898Shi C H,Zheng Y F,Wu F F,et al.Effects of simulated elevated atmospheric O3concentration on the quantity of microorganisms in winter wheat rhizospheric and non-rhi-zospheric soils[J].Acta Pedologica Sinica,2009,46(5):894-898(in Chinese)
• [16]刘水长.我国稻米需求和生产情况[J].粮食问题研究,2005(3):16-18Liu S C.Production and demand of rice in China[J].Grain Issues Research,2005,(3):16-18(in Chinese)
• [17]Taehibana S,Konishi N.Diurnal variation of in vivo andin vitro reductase activity in cucumber plants[J].Journal of the Japanese Society for Horticultural Science,1991,60(3):593-599
• [18]吕伟仙,葛滢,吴建之,等.植物中硝态氮、氨态氮、总氮测定方法的比较研究[J].光谱学与光谱分析,2004,24(2):204-206Lv W X,Ge Y,Wu J Z,et al.Study on the method for the determination of nitric nitrogen,ammoniacal nitrogen and total nitrogen in plant[J].Spectroscopy and Spectral Analysis,2004,24(2):204-206(in Chinese)
• [19]李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:258-260Li H S,Sun Q,Zhao S J.The Experimental Principle and Technology of Plant Physiology[M].Beijing:Higher Ed-ucation Press,2000:258-260(in Chinese)
• [20]Brehe J,Burch H.Enzymatic assay for glutathione[J].Analytical Biochemistry,1976,74(1):189-197
• [21]白月明,郭建平,刘玲,等.臭氧对水稻叶片伤害、光合作用及产量的影响[J].气象,2001,27(6):17-22Bai Y M,Guo J P,Liu L,et al.Influences of O3on the leaf injury photosynthesis and yield of rice[J].Meteoro-logical Monthly,2001,27(6):17-22(in Chinese)
• [22]董志强,解振兴,薛金涛,等.苗期叶面喷施6-BA对玉米硝酸还原酶活力的影响[J].玉米科学,2008,16(5):54-57Dong Z Q,Xie Z X,Xue J T,et al.The effect of6-BA on NRA of corn seedling by leaf spraying[J].Journal of Maize Sciences,2008,16(5):54-57(in Chinese)
• [23]Johnson D,Cheng W,Ball J.Effects of CO2and N ferti-lization on decomposition and N immobilization in pon-derosa pine litter[J].Plant and Soil,2000,224(1):115-122
• [24]门中华,李生秀.CO2浓度对冬小麦氮代谢的影响[J].中国农业科学,2005,38(2):320-326Men Z H,Li S X.Effect of CO2concentration on nitro-gen metabolism of winter wheat[J].Scientia Agricultura Sinica,2005,38(2):320-326(in Chinese)
• [25]伍文,黄益宗,李明顺,等.O3浓度升高对麦田土壤氨氧化细菌、氨氧化古菌和硝化细菌数量的影响[J].农业环境科学学报,2012,31(3):491-497Wu W,Huang Y Z,Li M S,et al.Effects of elevated o-zone on soil ammonium oxidation bacteria,ammonium ox-idation ancient bacteria and nitrobacteria in wheat field[J].Journal of Agro-Environment Science,2012,31(3):491-497(in Chinese)
• [26]李全胜,林先贵,胡君利,等.近地层臭氧浓度升高对稻田土壤氨氧化与反硝化细菌活性的影响[J].生态环境学报,2010,19(8):1789-1793Li Q S,Lin X G,Hu J L,et al.Elevated surface O3con-centration effects on soil ammonia-oxidizing and denitrif-ying bacterial activities in a rice field[J].Ecology and En-vironmental Sciences,2010,19(8):1789-1793(in Chi-nese)
• [27]Teri K,Palojarvi A,Ramo K,et a1.A3-year exposure to CO2and O3induced minor changes in soil N cycling in a meadow ecosystem[J].Plant and Soil,2006,286(1/2):61-73
• [28]郑有飞,石春红,吴芳芳,等.大气臭氧浓度升高对冬小麦根际土壤酶活性的影响[J].生态学报,2009,29(8):4386-4391Zheng Y F,Shi C H,Wu F F,et a1.Effects of simulated elevated atmospheric O3concentration on soil enzyme ac-tivity in winter wheat rhizospheric[J].Acta Ecologica Sinica,2009,29(8):4386-4391(in Chinese)
• [29]Luwe M W F,Takahama U,Heber U.Role of ascorbate in detoxifying ozone in the apoplast of spinach(Spinacia oleraceaL.)leaves[J].Plant Physiology,1993,101(3):969-976
• [30]Castillo F J,Greppin H.Extracellular ascorbic acid and enzyme-activities related to ascorbic acid metabolism in Sedum album L.leaves after ozone exposure[J].Environ-mental and Experimental Botany,1988,28(3):231-238
• [31]Tanaka K,Saji H,Kondo N.Immunological properties of spinach glutathione-reductase and inductive biosynthesis of the enzyme with ozone[J].Plant and Cell Physiology,1988,29(4):637-642
• [32]Mahalingam R,Jambunathan N,Gunjan S,et al.Analysis of oxidative signalling induced by ozone in Arabidopsis thaliana[J].Plant,Cell&Environment,2006,29(7):1357-1371