7种金属离子对中国林蛙和中华大蟾蜍蝌蚪的急性毒性比较研究Comparison Study on the Acute Toxicity of 7 Metal Ions on the Rana chensinensis and Bufo gargarizans Tadpoles
马丽;吴民耀;王宏元;
摘要(Abstract):
为评估Cu2+,Hg2+,Cr6+,Cd2+,Li+,Al3+和Co2+7种金属离子对中国林蛙(Rana Chensinensis)和中华大蟾蜍(Bufo gargarizans)蝌蚪的急性毒性效应,采用生物毒性试验方法对中国林蛙和中华大蟾蜍36期蝌蚪,进行上述7种金属离子的急性毒性试验,分别测定了这7种金属离子对中国林蛙蝌蚪和中华大蟾蜍蝌蚪的半数致死浓度(LC50)。此外,分析了中国林蛙和中华大蟾蜍36期蝌蚪的肥满度、肝指数等形态指标。结果显示,Cu2+、Hg2+、Cr6+、Cd2+、Li1+、Al3+、Co2+对中国林蛙蝌蚪的96h-LC50分别为0.270 mg·L-1、0.803 mg·L-1、2.375 mg·L-1、7.351 mg·L-1、11.273 mg·L-1、17.265 mg·L-1和20.973 mg·L-1。对中华大蟾蜍蝌蚪的96 h-LC50分别0.593 mg·L-1、0.593 mg·L-1、2.827 mg·L-1、2.592 mg·L-1、12.656 mg·L-1、14.020 mg·L-1和57.435 mg·L-1。中国林蛙蝌蚪对Cu2+、Cr6+、Li+、Co6+4种金属离子的敏感性相对较高,而中华大蟾蜍蝌蚪对Hg2+、Cd2+、Al3+3种金属离子的敏感性相对较高。形态指标的差异是中国林蛙与中华大蟾蜍蝌蚪对同一金属离子敏感性差异的原因之一。
关键词(KeyWords): 中国林蛙蝌蚪;中华大蟾蜍蝌蚪;金属离子;LC50
基金项目(Foundation): “秦巴山区可持续发展协同创新中心”资助
作者(Author): 马丽;吴民耀;王宏元;
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参考文献(References):
- [1]彭玉龙,王永敏,覃蔡清,等.重庆主城区降水中重金属的分布特征及其沉降量[J].环境科学,2014,35(7):2490-2496Peng Y L,Wang Y M,Qin C Q,et al.Concentrations and deposition fluxes of heavy metals in precipitation in core urban areas,Chongqing[J].Environment Science,2014,35(7):2490-2496(in Chinese)
- [2]Toppi L S,Gabbrielli R.Response to cadmium in higher plants[J].Environmental and Experimental Botany,1999,41(2):105-130
- [3]黄益宗,郝晓伟,雷鸣,等.重金属污染土壤修复技术及其修复实践[J].农业环境科报,2013,32(3):409-417Huang Y Z,Hao X W,Lei M,et al.The remediation technology and remediation practice of heavy metals contaminated soil[J].Journal of Agro-Environment Science,2013,32(3):409-417(in Chinese)
- [4]Chen T H,Gross J A,Karasov W H.Adverse effects of chronic copper exposure in larval northern leopard frogs(RANA PIPIENS)[J].Environmental Toxicology and Chemistry,2007,26(7):1470-1475
- [5]Lv Y T.Effect of zinc on the growth and development of larvae of bay scallop argopecten irradians[J].Chinese Journal of Oceanology and Limnology,1988,7(4):318–326
- [6]Ramachandran S,Patel T R,Colbo M H.Effect of copper and cadmium on three Malaysian tropical estuarine invertebrate larvae[J].Ecotoxicology and Environmental Safety,1997,36(2):183–188
- [7]Ricardo B,Edouard H,Matthias N L.Seaman.Effects of storage temperature and duration on toxicity of sediments assessed by the Crassostrea gigas oyster embryo bioassay[J].Environmental Toxicology and Chemistry,1998,17(10):2100–2105
- [8]柴丽红,王宏元,吴民耀,等.氟对不同发育时期中华大蟾蜍蝌蚪的急性毒性研究[J].安全与环境学报,2013,13(4):5-9Chai L H,Wang H Y,Wu M Y,et al.On the acute toxicity of fluoride to the larvae of Bufo gargarizans at different developmental stages[J].Journal of Safety and Environment,2013,13(4):5-9(in Chinese)
- [9]Kerby J L,Richards-Hrdlicka K L,Storfer A,et al.An examination of amphibian sensitivity to environmental contaminants:are amphibians poor canaries[J].Ecology Letters,2010,13(1):60-67
- [10]Hopkins W A.Amphibians as models for studying environmental change[J].ILAR Journal,2007,48(3):270-277
- [11]秦晓飞,秦占芬,徐晓白.前哨动物在环境污染物人体健康风险评价中的应用[J].生态毒理学报,2007,2(4):476-480Qin X F,Qin Z F,Xv X B.Application of sentinel animals to human health risk assessment of environmental contaminants[J].Asian Journal of Ecotoxicology,2007,2(4):270-277(in Chinese)
- [12]Gosner K L.A simplified table for staging anuran embryos and larvae with notes on identification[J].Herpetologica,1960,16(3):183-190
- [13]Sarah S,Bina Perl R G,Anna R,et al.Larval mor-phology and development of the Malagasy frog Mantidactylus betsileanus[J].Salamandra,2013,49(4):186-200
- [14]廉静,李丕鹏,陆宇燕,等.辽宁产中华大蟾蜍和花背蟾蜍蝌蚪形态特征的比较和分析[J].四川动物,2009,28(4):499-504Lian J,Li P P,Lu Y Y,et al.Comparison and analysis of tadpoles between Bufo gargarizans and B.raddei from Liaoning[J].Sichuan Journal of Zoology,2009,28(4):499-504(in Chinese)
- [15]Saka M.Examination of an amphibian-based assay using the larvae of Xenopus laevis and Ambystoma mexicanum[J].Ecotoxicology and Environmental Safety,2003,55(1):38-45
- [16]刘静,柴丽红,吴民耀,等.中华蟾蜍蝌蚪变态过程中甲状腺的组织学变化[J].西北农林科技大学学报:自然科学版,2012,40(3):7-12Liu J,Chai L H,Wu M Y,et al.Changes of thyroid gland histological structures during the metamorphosis of Bufo gargarizans tadpole[J].Journal of Northwest A&F University(Natural Science Edition),2012,40(3):7-12(in Chinese)
- [17]Mc Grath P,Seng W L,Willett C.et al.Determination of LD50and assessment of drug induced developmental toxicity in zebrafish[J].Journal of Pharmacological and Toxicological Methods,2008,58(2):150-150
- [18]Han S S,Chow W K.Calculating FED and LC50for testing toxicity of materials in bench-scale tests with a cone calorimeter[J].Polymer Testing,2005,24(7):920-924
- [19]周永欣,章宗涉.水生生物毒性试验方法[M].北京:中国农业出版社,1989Zhou Y X,Zhang Z S.Methods of Toxicity Test to Aquatic Organisms[M].Beijing:China Agriculture Press,1989(in Chinese)
- [20]李春瑜.重金属铜、镉对海陆蛙蝌蚪毒性作用的初步研究[D].海南师范大学,2013Li C Y.Preliminary study on toxicity of copper and cadmium on Fejervaya cancrivora tadpoles[D].Hai Nan Normal University,2013(in Chinese)
- [21]Sunita R N,John L,Fitzpatrick N F,et al.Toxicity of dissolved Cu,Zn,Ni and Cd to developing embryos of the blue mussel(Mytilus trossolus)and the protective effect of dissolved organic carbon[J].Comparative Biochemistry and Physiology,Part C:Toxicology&Pharmacology,2009,149(3):340-348
- [22]Martin M,Osborn K E,Billig P,et al.Toxicities of ten metals to Crassostrea gigas and Mytilus edulis embryos and Cancer magister larvae[J].Marine Pollution Bulletin,1981,12(9):305-308
- [23]王爱国,陈学敏,鲁文清,等.低硒高镉对大鼠组织中谷胱甘肽过氧化物酶活性影响的动态观察[J].中国地方病学杂志,1995,14(6):337-340Wang A G,Chen X M,Lu W Q,et al.Dynamie observation of effect of low-dose selenium and high-dose cadmium on activity of Glutathione Peroxiase in rat tissues[J].Chinese Journal of Endemiology,1995,14(6):337-340(in Chinese)
- [24]Vogiatzis A K,Loumbourdis N S.A study of glycogen,lactate,total fats,protein,and glucose concentration in the liver of the frog Rana ridibunda,after exposure to cadmium for 30 days[J].Environmental Pollution,1999,104(3):335-340
- [25]Papadimitriou E,Loumbourdis N S.Exposure of the frog Rana ridibunda to copper:Impacton two biomarkers,lipid peroxidation and glutathione[J].Bulletin of Environmental Contamination and Toxicology,2002,69(6):885-891
- [26]Roy J I.Environmental contaminants encyclopedia chromium(in general)entry[EB/OL].National park service(1997-07-01)[1998-03-01].http://www.nature.nps.gov/hazardssafety/toxic/index.cfm
- [27]Khangarot B.S,Ray P.K.Sensitivity of toad tadpoles,Bufo melanostictus(Schneider)to heavy metals[J].Bulletin of Environmental Contamination and Toxicology,1987,38(3):523-527
- [28]石戈,王健鑫,武佳,等.Cu2+和Cr6+对中国林蛙蝌蚪的急性毒性[J].东北师大学报(自然科学版),2007,39(2):116-121Shi G,Wang J X,Wu J,et al.Acute toxicity of Cu2+and Cr6+to Rana chensinensis tadpoles[J].Journal of Northeast Normal University(Natural Science Edition),2007,39(2):116-121(in Chinese)
- [29]王寿兵,郭锐,屈云芳,等.Cu对中国林蛙蝌蚪的急性毒性[J].应用生态学报,1998,9(3):309-312Wang S B,Guo R,Qu Y F,et al.Acute toxicity of Cu2+to Rana chensinensis tadpole[J].Chinese Journal of Applied Ecology,1998,9(3):309-312
- [30]卢祥云,张燕萍,吴海东,等.汞离子和铜离子对中华大蟾蜍蝌蚪联合毒性研究[J].四川动物,2006,25(2):379-381Lu X Y,Zhang Y P,Wu H D,et al.Study on joint toxicity of mercury ion and copper ion to Bufo gargarizans tadpole[J].Sichuan Journal of Zoology,2006,25(2):379-381(in Chinese)
- [31]Li X Y,Xiao N,Zhang,Y H.Toxic effects of octylphenol on the expression of genes in liver identified by suppression subtractive hybridization of Rana chensinensis[J].Ecotoxicology,2014,23(1):1-10
- [32]Formicki G,Stawarz R,Lukac N,et al.Combined effects of cadmium and ultraviolet radiation on mortality and mineral content in common frog(Rana temporaria)larvae[J].Journal of Environmental Science and Health.Part A:Toxic/Hazardous substances&Environmental Engineering,2008,43(10):1174-1183
- [33]马瑜,李勃,张育辉.敌百虫对中国林蛙蝌蚪生长发育的毒性效应[J].生态毒理学报,2014,9(3):531-537Ma Y,Li B,Zhang Y H.Toxicity effect of trichlorfon on the growth and development of tadpoles Rana chensinensis[J].Asian Journal of Ecotoxicology,2014,9(3):531-537(in Chinese)
- [34]Ken O,Osamu T,Akihiko K,et al.Metal ion-responsive transgenic Xenopus laevis as an environmental monitoring animal[J].Environmental Toxicology and Pharmacology,2003,13(3):153-161
- [35]Marcantonio A S,Tavares R M J,Franca F M,et al.Toxicity of zinc sulphate for tadpoles of bullfrogs(Lithobates catesbeianus):acute toxicity,chronic toxicity and hematological parameters[J].Boletim do Instituto de Pesca,2011,37(2):143-154
- [36]李勃,王维君,李忻怡,等.铬对中国林蛙蝌蚪生长发育的毒性效应[J].生态学杂志,2013,32(3):648-654Li B,Wang W J,Li X Y,et al.Toxic effect of Chromium on the growth and development of Rana Chensinensis tadpoles[J].Chinese Journal of Ecology,2013,32(3):648-654(in Chinese)
- [37]Phillip L W,Robert C J,Stephen M R,et al.Principles of Toxicology[M].The United States of America:A Wiley-Interscience Publication,2000:325-343