李娟英;李振华;崔昱;胡谦;陈美娜;郑奕兴;

• 1:水产动物遗传育种中心上海市协同创新中心

摘要(Abstract)：

菊酯类农药已广泛用于农用、卫生、渔业等领域,因高疏水性累积于沉积物中的此类污染物的生物有效性评价对环境风险研究具有重要意义。采用PDMS(聚二甲基硅氧烷)高聚物作为固相微萃取材料,基于热力学平衡微损耗性方法测定了沉积物孔隙水中高效氯氟氰菊酯、联苯菊酯、氯菊酯和甲氰菊酯的自由溶解态浓度(Cfree);同时将PDMS上菊酯类农药的浓度与菲律宾蛤仔体内生物累积浓度的相关性进行了比较研究。结果表明,该方法可以准确测定菊酯类农药在沉积物孔隙水中的Cfree(23.3~255 ng·L-1)并用于评价此类物质(菊酯类农药)的生物有效性;菲律宾蛤仔对四种菊酯类农药的富集系数(BCF)为27.8~301,相对较小;菊酯类农药在PDMS上的浓度和菲律宾蛤仔体内的浓度满足lg Cb,lip=11.64lg CPDMS-51.29(R2=0.980)的定量关系,相关性极显著(P=0.009)。但生物—沉积物富集系数(BSAF)和PDMS-沉积物富集系数(PSAF)的分析比较发现,基于PDMS和生物体内菊酯浓度极显著相关的仿生应用还有待污染物在菲律宾蛤仔体内生物转化数据的进一步补充和校正。

关键词(KeyWords)： 菊酯类农药;沉积物;生物有效性;聚二甲基硅氧烷;菲律宾蛤仔

Abstract:

Keywords:

基金项目(Foundation): 上海海洋大学科技发展专项资助(A2-0209-14-200055);; 农业部淡水水产种质资源重点实验室开放课题

作者(Author): 李娟英;李振华;崔昱;胡谦;陈美娜;郑奕兴;

Email:

参考文献(References)：

• [1]叶玫,姜琳琳,余颖,等.气相色谱法测定水产养殖区表层沉积物中拟除虫菊酯残留量[J].渔业科学进展,2012,33(5):109-115Ye M,Jiang L L,Yu Y,et al.Determination of pyrethroids residues in surface sediment from aquaculture area by gas chromatography[J].Progress in Fishery Sciences,2012,33(5):109-115(in Chinese)
• [2]黄向丽.草鱼体内菊酯类杀虫剂的残留与消除规律研究[D].西南大学,2009Huang X L.The studies on determination ofpyrethroid pesticides residues and their residual eliminations in grass carp[D].Southwest University,2009(in Chinese)
• [3]谢湘云.海洋环境样品中痕量有机氯和拟除虫菊酯农药的测定方法[D].厦门大学,2007Xie X Y.Determination method of the trace organochlorine and pyrethroid pesticide in marine environmental samples[D].Xiamen University,2007(in Chinese)
• [4]Zhao D,Liu X,Shi W,et al.Determination of cypermethrin residues in crucian carp tissues by MSPD/GCECD[J].Chromatographia,2011,73:1021-1025
• [5]杨琳,温裕云,弓振斌.加速溶剂萃取-液相色谱-串级质谱法测定近岸及河口沉积物中的拟除虫菊酯农药[J].分析化学研究报告,2010,38(7):968-972Yang L,Wen Y Y,Gong Z B.Determination of pyrethroid pesticides in estuarine and coastal sediments byaccelerated solvent extraction and liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Analytical Chemistry,2010,38(7):968-972(in Chinese)
• [6]Lao W,Tiefenthaler L,Greenstein D J,et al.Pyrethroids in southern California coastal sediments[J].Environmental Toxicology and Chemistry,2012,31(7):1649-1656
• [7]You J,Harwood A D,Li H,et al.Chemical techniques for assessing bioavailability of sediment-associated contaminants:SPME versus Tenax extraction[J].Journal of Environmental Monitoring,2011,13(4):792-800
• [8]胡霞林,刘景富,卢士燕,等.环境污染物的自由溶解态浓度与生物有效性[J].化学进展,2009,21(2):514-523Hu X L,Liu J F,Lu S Y,et al.Freely dissolved concentration and bioavailability of environmental pollutants[J].Progress in Chemistry,2009,21(2):514-523(in Chinese)
• [9]Cornelissen G,Pettersen A,Broman D,et al.Field testing of equilibrium passive samplers to determine freely dissolved native polycyclic aromatic hydrocarbon concentrations[J].Environmental Toxicology and Chemistry,2008,27(3):499-508
• [10]Lee J N,Park C,Whitesides G M.Solvent compatibility of poly(dimethylsiloxane)-based microfluidic devices[J].Analytical Chemistry,2003,75(23):6544-6554
• [11]Amanda D H,Peter F L,Michael J L.Can SPME fiber and Tenax methods predict the bioavailability of biotransformed insecticides[J].Environmental Science&Technology,2012,46(4):2413-2419
• [12]Hunter W,Xu Y,Spurlock F,et al.Using disposable polydimethylsiloxane fibers to assess the bioavailability of permethrin in sediment[J].Environmental Toxicology and Chemistry,2008,27(3):568-75
• [13]You J,Brennan A,Lydy M J.Bioavailability and biotransformation of sediment-association pyrethroid insecticides in Lumbriculus variegates[J].Chemosphere,2009,75:1477-1482
• [14]夏欢.应用固相微萃取技术测定聚二甲基硅氧烷/水分配系数及沉积物孔隙水中自由溶解态疏水性有机物[D].中国科学院广州地球化学研究所,2007Xia H.Applications of solid-phasemicroextraction in measuring partition coefficients between poly(dimethy)siloxane-coated fiber and water and freely dissolved concentration of hydrophobic organic chemicals in sediment porewater[D].Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,2007(in Chinese)
• [15]方淑红,陈鹏,卞京娜,等.太湖及辽河流域表层沉积物中拟除虫菊酯的浓度水平及毒性评估[J].环境科学学报,2012,32(10):2600-2606Fang S H,Chen P,Bian J N,et al.Levals and toxicity assessment of pyrethroids in the surface sediments of Taihu Lake and Liaohe River[J].Acta Scientiae Circumstantiae,2012,32(10):2600-2606(in Chinese)
• [16]李天云.利用河蚬研究沉积物中有机氯农药和多环芳烃在生物体内的累积效应[D].西南大学,2008Li T Y.Bioaccumulation of organochlorine pesticides(OCPs)and polycyclic aromatic hydrocarbons(PAHs)for corbicula flumina from sediment in sewer channel of Tianjin[D].Southwest University,2008(in Chinese)
• [17]Li J,Tang J Y M,Jin L,et al.Undestanding bioavailbility and toxicity of sediment-associated contaminants by combining passive sampling with in vitro bioassays in an urban river catchment[J].Environmental Toxicology and Chemistry,2013,32(12):2888-2896
• [18]Ding Y,You Y,Lydy M J.Analysis of pyrethroid insecticides in Chironomus dilutes using matrix solid phase dispersion extraction[J].Bulletin of Environmental Contamination Toxicology,2009,83(3):388-392
• [19]Hunter W,Yang Y,Reichenberg F,et al.Measuring pyrethroids in sediment pore water using matrix-solid phase microextraction[J].Environmental Toxicology and Chemistry,2009,28(1):36-43
• [20]胡霞林,刘景富.微损耗固相微萃取技术及其在有机物自由溶解态浓度测定中的应用[J].环境化学,2011,30(1):252-262Hu X L,Liu J F.Negligible depletion solid-phasemicroextraction and its application in the determination of freely dissolved concentration of organic pollutants[J].Environmental Chemistry,2011,30(1):252-262(in Chinese)
• [21]Pistocchi A,Vizcaino P,Hauck M.A GIS modelbased screening of potential contamination of soil and water by pyrethroids in Europe[J].Journal of Environmental Management,2009,90(11):3410-3421
• [22]Sabljic A,Gusten H,verhaar H,et al.QSAR modeling of soil sorption-improvements and systemations of log Koc vs log Kow correlation[J].Chemosphere,1995,31:4489-4514
• [23]He L M,Troiano J,Wang A,et al.Environmental chemistry,ecotoxicity,and fate of lambda-cyhalothrin[J].Reviews of Environmental Contamination and Toxicology,2008,195:71-91
• [24]Katagi T.Environmental behavior of synthetic pyrethroids[J].Topics in Current Chemistry,2012,314:167-202
• [25]Feo M L,Ginebreda A,Eljarrat E,et al.Presence of pyrethroid pesticides in water and sediments of Ebro River Delta[J].Journal of Hydrology,2010,393:156-162
• [26]姚书春,孙成,陈达,等.拟除虫菊酯类化合物的QSAR研究[J].中国环境科学,2001,21(4):309-312Yao S C,Sun C,Chen D,et al.Study on quantitative structure-relationships forpyrethroid pesticides compounds[J].China Environmental Science,2001,21(4):309-312(in Chinese)
• [27]You J,Pehkonen S,Landrum P F,et al.Desorption of hydrophobic compounds from laboratory-spiked sediments measured by tenax absorbent and matrix solidphase microextraction[J].Environmental Science&Technology,2007,41(16):5672-5678
• [28]Jia F,Gan J.Comparing black carbon types in sequestering polybrominated diphenyl ethers(PBDEs)in sediments[J].Environmental Pollution,2014,184:131-137
• [29]徐晓阳,孙红文,张志远.半透膜微萃取方法的建立及其在测定土壤间隙水中憎水性有机污染物的应用[J].农药环境科学学报,2010,29(4):801-805Xu X Y,Sun H W,Zhang Z Y.Semi-permeable membrance based micro-extraction technique and its application in determination of hydrophobic organic contaminants in soil pore-water[J].Journal of Agro-Environment Science,2010,29(4):801-805(in Chinese)
• [30]耿雪冰.溴氰菊酯残留的检测方法及对河蟹的毒性研究[D].南京农业大学,2010Geng X B.Studies on detection method and toxicity of deltamethrin in river crabs,Eriocheir sinensis[D].Nanjing Agricultural University,2010(in Chinese)
• [31]沈坚.三种农药对底栖软体动物的毒性效应和生物富集性研究[D].浙江大学,2013Shen J.The toxicity and bio-concentration of three pesticides to benthicanimals mollusks[D].Zhejiang University,2013(in Chinese)
• [32]Ding Y,Weston D P,You J,et al.Toxicity of sediment-associated pesticides to Chironomus dilutes and Hyalella azteca[J].Archives Environmental Contamination Toxicology,2011,61(1):83-92
• [33]Fojut T F,Palumbo A J,Tjeerdema R S.Aquatic life water quality criteria derived via the UC Davis Method:Ⅱ.Pyrethroid insecticides[J].Reviews of Environmental Contamination and Toxicology,2012,216:51-103
• [34]李玲玉,刘艳,颜冬云,等.拟除虫菊酯类农药的降解与代谢研究进展[J].环境科学与技术,2010,33(4):65-71Li L Y,Liu Y,Yan D Y,et al.Research progress on degradation and metabolism of pyrethroid insecticides[J].Environmental Science&Technology,2010,33(4):65-71(in Chinese)
• [35]Pehkonen S,You J,Akkanen J,et al.Influence of black carbon and chemical planarity on bioavailability of sediment-associated contaminants[J].Environmental Toxicology and Chemistry,2010,29(9):1976-1983
• [36]王帅.罗非鱼及水体中拟除虫菊酯类农药残留量的测定[D].中南林业科技大学,2013Wang S.The study on determination of pyrethroid pesticide residues in tilapia and water[D].Central South University of Forestry&Technology,2013(in Chinese)
• [37]Escher B I,Hermens J L.Modes of action in ecotoxicology:Their role in body burdens,species sensitivity,QSARs,and mixture effect[J].Environmental Science&Technology,2002,36(20):4201-4217
• [38]Ding Y,Landrum P F,You J,et al.Assessing bioavailability and toxicity of permethrin and DDT in sediment using matrix solid phase microextraction[J].Ecotoxicology,2013,22:109-117
• [39]徐斐,赵洁,曹慧,等.拟除虫菊酯类农药的酶水解研究进展[J].东北农业大学学报,2012,43(7):1-5Xu F,Zhao J,Cao H,et al.Degradation of pyrethroid insecticides catalyzed by enzymes[J].Journal of Northeast Agricultural University,2012,43(7):1-5(in Chinese)
• [40]You J,Landrum P F,Lydy M J.Comparison of Chemical approaches for assessing bioavailability of sedimentassociated contaminants[J].Environmental Science&Technology,2006,40(20):6348-6353