碲化镉量子点(CdTe QDs)对肝细胞的毒性效应及线粒体介导的毒性机制研究Study on the Toxicity and Mitochondrial-Mediated Mechanism of Two Hepatocytes Induced by CdTe QDs
陆杰;姚影;汪岩;臧一腾;瞿靖;何克宇;吴添舒;梁雪;魏婷婷;熊丽林;张婷;唐萌;
摘要(Abstract):
本文探讨了碲化镉量子点(CdTe QDs)对肝细胞的毒性效应及其影响因素,为探索量子点的肝毒性机制提供一定依据。采用人肝癌细胞(Hep G2)和人正常肝细胞(L02)为细胞模型,设置0、25、50和100μmol·L-14个浓度组,采用CCK-8法检测细胞生存率,石墨炉法检测细胞内镉元素含量,采用流式细胞术,装载荧光探针DCFH-DA检测细胞内活性氧水平,采用FITC/PI检测细胞凋亡以及JC-1检测细胞ATP水平。研究结果显示:CdTe QDs诱导2种肝细胞生存率降低,细胞凋亡率升高,细胞对QDs的摄入水平具有时间依赖性,细胞内活性氧水平显著升高,线粒体膜电位降低和ATP含量显著减少,且2种肝细胞比较发现L02细胞损伤程度更为严重。CdTe QDs对2种肝细胞造成损伤,对L02细胞损伤更明显,其原因是L02细胞对CdTe QDs摄取更多,导致进入细胞的QDs引发更为严重的损伤效应。
关键词(KeyWords): 碲化镉量子点;肝细胞;线粒体损伤;细胞凋亡;活性氧
基金项目(Foundation): 国家自然科学基金项目(No.31671034,81673218,81473003,81302461,81502783,81573186);; 国家重大科学研究计划项目(No.2011CB933404)
作者(Author): 陆杰;姚影;汪岩;臧一腾;瞿靖;何克宇;吴添舒;梁雪;魏婷婷;熊丽林;张婷;唐萌;
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