陆杰;姚影;汪岩;臧一腾;瞿靖;何克宇;吴添舒;梁雪;魏婷婷;熊丽林;张婷;唐萌;

• 1:环境医学工程教育部重点实验室东南大学公共卫生学院苏州纳米科技协同创新中心
• 2:江苏省生物材料与器件重点实验室东南大学

摘要(Abstract)：

本文探讨了碲化镉量子点(CdTe QDs)对肝细胞的毒性效应及其影响因素,为探索量子点的肝毒性机制提供一定依据。采用人肝癌细胞(Hep G2)和人正常肝细胞(L02)为细胞模型,设置0、25、50和100μmol·L-14个浓度组,采用CCK-8法检测细胞生存率,石墨炉法检测细胞内镉元素含量,采用流式细胞术,装载荧光探针DCFH-DA检测细胞内活性氧水平,采用FITC/PI检测细胞凋亡以及JC-1检测细胞ATP水平。研究结果显示:CdTe QDs诱导2种肝细胞生存率降低,细胞凋亡率升高,细胞对QDs的摄入水平具有时间依赖性,细胞内活性氧水平显著升高,线粒体膜电位降低和ATP含量显著减少,且2种肝细胞比较发现L02细胞损伤程度更为严重。CdTe QDs对2种肝细胞造成损伤,对L02细胞损伤更明显,其原因是L02细胞对CdTe QDs摄取更多,导致进入细胞的QDs引发更为严重的损伤效应。

关键词(KeyWords)： 碲化镉量子点;肝细胞;线粒体损伤;细胞凋亡;活性氧

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.31671034,81673218,81473003,81302461,81502783,81573186);; 国家重大科学研究计划项目(No.2011CB933404)

作者(Author): 陆杰;姚影;汪岩;臧一腾;瞿靖;何克宇;吴添舒;梁雪;魏婷婷;熊丽林;张婷;唐萌;

Email:

DOI:

参考文献(References)：

• [1]Bruchez M J,Moronne M,Gin P,et al.Semiconductor nanocrystals as fluorescent biological labels[J].Science,1998,281(5385):2013-2016
• [2]Leutwyler W K,Bürgi S L,Burgl H.Semiconductor clusters,nanocrystals,and quantum dots[J].Science,1996,271(5251):933-937
• [3]Manshian B B,Soenen S J,Al-Ali A,et al.Cell type-dependent changes in Cd Se/Zn S quantum dot uptake and toxic e Endpoints[J].Toxicology Science,2015,144(2):246-258
• [4]Brunetti V,Chibli H,Fiammengo R,et al.In P/Zn S as a safer alternative to Cd Se/Zn S core/shell quantum dots:In vitro and in vivo toxicity assessment[J].Nanoscale,2013,5:307-317
• [5]Chong Y,Maa Y F,Shen H,et al.The in vitro and in vivo toxicity of graphene quantum dots[J].Biomaterials,2014,35:5041-5048
• [6]Barroso M M.Quantum dots in cell biology[J].Journal of Histochemistry and Cytochemistry,2011,59(3):237-251
• [7]Fitzpatrick J A J,Andreko S K,Ernst L A,et al.Longterm persistence and spectral blue shifting of quantum dots in vivo[J].Nano Letters,2009,9(7):2736-2741
• [8]Hauck T S,Anderson R E,Fischer H C,et al.In vivo quantum-dot toxicity assessment[J].Small,2010,6(1):138-144
• [9]Su Y,Peng F,Jiang Z,et al.In vivo distribution,pharmacokinetics,and toxicity of aqueous synthesized cadmiumcontaining quantum dots[J].Biomaterials,2011,32(25):5855-5862
• [10]Liu W,Zhang S,Wang L,et al.Cd Se quantum dot(QD)-induced morphological and functional impairments to liver in mice[J].PLo S ONE,2011,6(9):e24406
• [11]Zhang T,Hu Y,Tang M,et al.Liver toxicity of cadmium telluride quantum dots(Cd Te QDs)due to oxidative stress in vitro and in vivo[J].International Journal of Molecular Sciences,2015,16(10):23279-23299
• [12]Ye L,Yong K T,Liu L,et al.A pilot study in non-human primates shows no adverse response to intravenous injection of quantum dots[J].Nature Nanotechnology,2012,7(7):453-458
• [13]陆杰,张婷,唐萌,等.碲化镉量子点细胞毒性研究进展[J].生态毒理学报,2016,11(5):24-31Lu J,Zhang T,Tang M,et al.Research advances in cytotoxicity of Cd Te quantum dots[J].Asian Journal of Ecotoxicology,2016,11(5):24-31(in Chinese)
• [14]Nguyen K C,Willmore W G,Tayabali A F.Cadmium telluride quantum dots cause oxidative stress leading to extrinsic and intrinsic apoptosis in hepatocellular carcinoma Hep G2 cells[J].Toxicology,2013,306:114-123
• [15]Lu Y H,Xu S C,Chen H Y,et al.Cd Se/Zn S quantum dots induce hepatocyte pyroptosis and liver inflammation via NLRP3 inflammasome activation[J].Biomaterials,2016,90:27-39
• [16]Zhan Q L,Tang M,Zhang T,et al.Effects of Cd Te@MPA quantum dots on RAW264.7 cells viability[J].Journal of Environment Health,2014,31(2):111-113
• [17]Breunig M,Bauer S,Goepferich A,et al.Polymers and nanoparticles:Inteligent tools for intracellular targeting[J].European Journal of Pharmaceutics and Biopharmaceutices,2008,68(1):112-128
• [18]Lin C H,Yang M H,Louis W,et al.Cd/Se/Te-based quantum dot 705 modulated redox homeostasis with hep-atotoxicity in mice[J].Nanotoxicology,2011,5(4):650-663
• [19]Wang M M,Wang J L,Sun H B,et al.Time-dependent toxicity of cadmium telluride quantum dots on liver and kidneys in mice:Histopathological changes with elevated free cadmium ions and hydroxyl radicals[J].International Journal of Nanomedicine,2016,11:2319-2328
• [20]Fan J J,Sun Y,Wang S F,et al.Inhibition of autophagy overcomes the nanotoxicity elicited by cadmium-based quantum dots[J].Biomaterials,2016,78:102-114
• [21]Su Y Y,Hub M,Fan C H,et al.The cytotoxicity of Cd Te quantum dots and the relative contributions from released cadmium ions and nanoparticle properties[J].Biomaterials,2010,31:4829-4834
• [22]Chen N,He Y,Su Y Y,et al.The cytotoxicity of cadmium-based quantum dots[J].Biomaterials,2012,33:1238-1244
• [23]Lemarie A,Lagadic G D,Morzadec C,et al.Cadium induces caspase-independent apoptosis in liver Hep3B cells:Role for calcium in signaling oxidative stress-relatived impairment of mitochondria and relocation of endonuclease G and apoptosis-inducing factor[J].Free Radical Biology Medicine,2004,36(12):1517-1531
• [24]Peng L,He M,Chen B B,et al.Cellular uptake,elimination and toxicity of Cd Se/Zn S quantum dots in Hep G2cells[J].Biomaterials,2013,34:9545-9558
• [25]Paesano L,Perotti A,Buschini A,et al.Markers for toxicity to Hep G2 exposed to cadmium sulphide quantum dots;damage to mitochondria[J].Toxicology,2016,374:18-28
• [26]Li Y B,Zhang H X,Guo C X,et al.Induction of thioglycolic acid-capped cadmium selenide quantum dots on hepatotoxicity and DNA damage in human hepatic cells[J].Journal of Jilin University:Medicine Edition,2012,38(3):495-500
• [27]Nguyen K C,Rippstein P,Azam F,et al.Mitochondrial toxicity of cadmium telluride quantum dot nanoparticles in mammalian hepatocytes[J].Toxicology Science,2015,146(1):31-42