陈晔;陈荣;莫正平;

• 1:厦门大学环境与生态学院近海海洋环境科学国家重点实验室

摘要(Abstract)：

为了解重金属污染对海洋鱼类热休克蛋白(HSPs)基因表达的影响,将褐菖鲉(Sebastiscus marmoratus)分别暴露于1.6、8、40、200、500μg·L~(-1)Cd、Pb溶液中,用环介导等温扩增技术(loop-mediated isothermal amplification,LAM P)定量检测褐菖鲉肝脏HSP60、HSP70、HSP90、HSC70 mRNA表达量。结果表明:Pb仅在40μg·L-1时显著抑制HSP60、HSP90、HSC70 mRNA表达量,8μg·L~(-1)时即可显著抑制HSP70 m RNA表达量,并在40μg·L~(-1)时达到最小值;Cd对HSP60、HSP90、HSC70的诱导不明显,但能显著诱导HSP70,并在500μg·L~(-1)时达到最大值。相比之下,褐菖鲉肝HSP70基因对重金属Cd、Pb污染较为敏感,有潜力成为监测海洋重金属污染的预警分子。

关键词(KeyWords)： Cd;Pb;褐菖鲉;肝脏;热休克蛋白;HSPs;环介导等温扩增

Abstract:

Keywords:

基金项目(Foundation): 海洋公益性项目"海洋污染生物效应快速监测与评价技术应用示范"子任务-海洋污染效应生物标志物技术集成与研发Ⅱ(201005016)

作者(Author): 陈晔;陈荣;莫正平;

Email:

DOI:

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