朱友芳;洪万树;林金忠;

• 1:福建莆田市水产科学研究所
• 2:厦门大学海洋与环境学院

摘要(Abstract)：

为给中国花鲈(Lateolabrax maculatus)养殖提供污染生物学的理论数据,研究了中国花鲈幼鱼的铜离子中毒症状和半致死浓度(LC50);检测了幼鱼肝脏的谷胱甘肽(GSH)和丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷丙转氨酶(GPT)和谷草转氨酶(GOT)活力;分析了铜离子胁迫对幼鱼的红细胞总核异率和微核率的影响。结果表明:铜离子对幼鱼24、48、72和96h的LC50分别为1.85、1.66、1.48和0.86mg·L-1。濒死的幼鱼肝脏组织溶解性坏死,鳃部组织出现大量的血窦,幼鱼肝脏的GSH和MDA含量及SOD、GPT和GOT活力对铜离子胁迫反应敏感,而CAT活力则反应不敏感。在铜离子浓度为0.42mg·L-1的浓度组中暴露96h,幼鱼的总核异率和微核率显著高于对照组(p<0.05)。以上结果说明铜离子胁迫损害了中国花鲈幼鱼肝脏的抗氧化系统、肝脏组织和鳃部组织,并具有一定的遗传毒性。

关键词(KeyWords)： 中国花鲈;铜离子;半致死浓度;肝脏组织;酶活力;微核测定

Abstract:

Keywords:

基金项目(Foundation): 福建省莆田市科技局项目[2009N15(5)]

作者(Author): 朱友芳;洪万树;林金忠;

Email:

DOI:

参考文献(References)：

• [1]姚志峰,章龙珍,庄平,等.铜对中华鲟幼鱼的急性毒性及对肝脏抗氧化酶活性的影响[J].中国水产科学,2010,17(4):731-738Yao Z F,Zhang L Z,Zhuang P,et al.Effects of antioxidant enzyme in liver and acute toxicity of Cu2+on juvenile Chinese sturgeon[J].Journal of Fishery Sciences of China,2010,17(4):731-738(in Chinese)
• [2]王利,汪开毓.鲤鱼亚急性铜中毒的病理组织学观察[J].中国兽医科学,2008,38(12):1089-1092Wang L,Wang K Y.Histopathological observation of Cyprinus carpio poisoned subacutely with copper[J].Chinese Veterinary Science,2008,38(12):10891092(in Chinese)
• [3]王利,汪开毓.硫酸铜诱发鲤鱼红细胞微核和核异常的研究[J].湖北农业科学,2008,47(11):1336-1337Wang L,Wang K Y.Effects of CuSO4on micronuclei and nuclear anomalies in Cyprinus carpio[J].Hubei Agricultural Sciences,2008,47(11):1336-1337(in Chinese)
• [4]金彩杏,丁跃平,刘士忠,等.三唑磷农药对鲈鱼等鱼类的急性毒性试验[J].水产科技情报,2002,29(4):156-158
• [5]郑伟刚,张兆琪,张美昭,等.盐度与碱度对花鲈幼鱼的毒性研究[J].中国生态农业学报,2005,13(3):116-118Zheng W G,Zhang Z Q,Zhang M Z,et al.Toxicity of salinity and alkalinity to Lateolabrax japonicus fingerlings[J].Chinese Journal of Eco-Agriculture,2005,13(3):116-118(in Chinese)
• [6]刘海燕,张伟,薛敏,等.三聚氰氨对花鲈的急性毒性实验研究[J].水生生物学学报,2009,33(2):157-163Liu H Y,Zhang W,Xue M,et al.Acute toxicity study for melamine on Japanese sea bass(Lateolabrax japonicus)[J].Acta Hydrobiologica Sinica,2009,33(2):157-163(in Chinese)
• [7]陈仪取,冯涛,郭祥群.3种农药对花鲈抗氧化酶和乙酰胆碱酯酶影响的研究[J].厦门大学学报(自然科学版),2004,43(6):828-832Chen Y Q,Feng T,Guo X Q.Effects of three kinds of pesticides on antioxidant enzymes and acetylcholinesterase activities of Lateolabrax japonicus[J].Journal of Xiamen University(Natural Science),2004,43(6):828-832(in Chinese)
• [8]Wu J F,Yu Z M,Song X X,et al.Comparative researches on effects of sodium dodecylbenzene sulfonate and sodium dodecyl sulfate upon Lateolabrax japonicus biomarker system[J].Environmental Toxicology and Pharmacology,2005,20(3):465-470
• [9]Wu J F,Yu Z M,Song X X,et al.Response of integrated biomarkers of fish(Lateolabrax japonicus)exposed to benzo[a]pyrene and sodium dodecylbenzene sulfonate[J].Ecotoxicology and Environmental Safety,2006,65(2):230-236
• [10]高蓉,张守刚,肖杭.脱氧鬼臼毒素对斑马鱼(Danio rerio)代谢酶和抗氧酶的活性影响[J].生态毒理学报,2009,4(5):700-704Gao R,Zhang S H,Xiao H.Effects of deoxypodophyllotoxin on activities of metabolic enzymes and antioxidant enzymes of Danio rerio[J].Asian Journal of Ecotoxicology,2009,4(5):700-704(in Chinese)
• [11]周永欣,章宗涉.水生生物毒性试验方法[M].北京:农业农业出版社,1989
• [12]王重刚,余群,郁昂,等.苯并(a)芘和芘暴露对梭鱼肝脏超氧化物歧化酶活性的影响[J].海洋环境科学,2002,21(4):10-13Wang C G,Yu Q,Yu A,et al.Effect of benzo(a)pyrene and pyrene exposure on hepatic superoxide dismutase in Mugilso-iuy[J].Marine Environmental Science,2002,21(4):10-13(in Chinese)
• [13]赵元凤,吕景才,宋晓阳,等.镉污染对鲢鱼超氧化物歧化酶和过氧化氢酶活性的影响[J].农业生物技术学报,2002,(10):267-271Zhao Y F,Lu J C,Song X Y,et al.Effect of cadmium on activities of superoxide dismutase and catalase in Aristchthys nobilis[J].Journal of Agricultural Biotechnology,2002,(10):267-271(in Chinese)
• [14]简乐,胡迪生.二硫化碳接触者血清超氧化物歧化酶与丙二醛水平观察[J].工业卫生与职业病,1999,25(3):142-144Jian L,Hu D S.Serum cuprozinc superoxide dismutase and malonyldialdehyde levels in carbon disulfide exposed workers[J].Industrial Health and Occupational Diseases,1999,25(3):142-144(in Chinese)
• [15]李赟,汝少国.久效磷对金鱼肝脏和血浆谷丙、谷草转氨酶活性的影响[J].安全与环境学报,2008,8(5):9-13Li Y,Ru S G.Effects of monocrotophos on GPTand GOT activities in the goldfish(Carassius auratus)livers and serum[J].Journal of Safety and Environment,2008,8(5):9-13(in Chinese)
• [16]De la Torre F R,Salibian A,Ferrari L.Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium[J].Environmental Pollution,2000,109(2):277-282
• [17]Ramaswamy M,Thangavel P,Selvam NP.Glutamic oxaloacetic transaminase(GOT)and glutamic pyruvic transaminase(GPT)enzyme activities in different tissues of Sarotherodon mossambicus(Peters)exposed to a carbamate pesticide,carbaryl[J].Pasticide Science,1999,55(12):1217-1221
• [18]刘红玲,于红霞,许铁莲,等.联苯胺类化合物对锦鲤肝脏转氨酶影响的初探[J].农业环境科学学报2005,24(6):1069-1074Liu H L,Yu H X,Xu T L,et al.Effects of benzidine congeners on two aminopherases in goldfish liver[J].Journal of Agro-Environment Science,2005,24(6):1069-1074(in Chinese)
• [19]Schmid W.The micronucleus test[J].Mutation Research,1975,31(1):9-15
• [20]龙静,张迎梅,朱丽娜,等.利用微核试验和彗星电泳试验评价黄河兰州段水质致遗传毒性作用[J].应用与环境生物学报,2006,12(1):59-63Long J,Zhang Y M,Zhu L N,et al.Evaluating genotoxicity of water quality in lanzhou section of the yellowriver using micronucleus test and comet assay[J].Chinese Journal of Applied&Environmental Biology,2006,12(1):59-63(in Chinese)
• [21]谢炎福,祖恩普.硫酸铜引起鱼类中毒原因的分析及对策[J].水利渔业,2005,25(6):98-99