铜离子对中国花鲈幼鱼的毒性研究Toxicity of Cu~(2+) to Juvenile Perch Lateolabrax maculatus
朱友芳;洪万树;林金忠;
摘要(Abstract):
为给中国花鲈(Lateolabrax maculatus)养殖提供污染生物学的理论数据,研究了中国花鲈幼鱼的铜离子中毒症状和半致死浓度(LC50);检测了幼鱼肝脏的谷胱甘肽(GSH)和丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷丙转氨酶(GPT)和谷草转氨酶(GOT)活力;分析了铜离子胁迫对幼鱼的红细胞总核异率和微核率的影响。结果表明:铜离子对幼鱼24、48、72和96h的LC50分别为1.85、1.66、1.48和0.86mg·L-1。濒死的幼鱼肝脏组织溶解性坏死,鳃部组织出现大量的血窦,幼鱼肝脏的GSH和MDA含量及SOD、GPT和GOT活力对铜离子胁迫反应敏感,而CAT活力则反应不敏感。在铜离子浓度为0.42mg·L-1的浓度组中暴露96h,幼鱼的总核异率和微核率显著高于对照组(p<0.05)。以上结果说明铜离子胁迫损害了中国花鲈幼鱼肝脏的抗氧化系统、肝脏组织和鳃部组织,并具有一定的遗传毒性。
关键词(KeyWords): 中国花鲈;铜离子;半致死浓度;肝脏组织;酶活力;微核测定
基金项目(Foundation): 福建省莆田市科技局项目[2009N15(5)]
作者(Author): 朱友芳;洪万树;林金忠;
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DOI:
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