铜镉复合胁迫下硫素对小麦幼苗铜镉吸收、亚细胞分布及毒性的影响Influence of Sulfur on Subcellular Distribution,Uptake and Toxicity of Cu and Cd to Wheat Seadlings
翁南燕;周东美;汪鹏;汪登俊;褚灵阳;
摘要(Abstract)：
采用水培的方法,研究了不同硫素供应水平对铜镉复合胁迫(Cu+Cd:10μmol.L-1+10μmol.L-1)下,小麦幼苗铜镉吸收、亚细胞分布及生理毒性的影响。结果表明,缺硫和高硫处理均降低了小麦幼苗根系和叶片中铜和镉的含量,不同硫水平处理对小麦幼苗根系亚细胞各组分中镉的分配比例没有产生影响,而对铜亚细胞组分的分配比例则产生了显著影响,表现为添加硫的处理细胞质组分分配比例增加,细胞壁组分的分配比例降低,缺硫处理则相反。铜镉复合胁迫对小麦幼苗产生明显的毒性作用,使其生长受到抑制、叶绿素含量降低、小麦幼苗MDA含量增加、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性降低以及还原性谷胱甘肽(GSH)含量降低。与适中硫处理相比,高硫处理能明显缓解铜镉复合胁迫对小麦的毒害,可以促进小麦幼苗生长,增加幼苗中叶绿素含量,提高SOD和CAT的活性,降低MDA在根系中的积累,提高根系GSH的含量,而缺硫处理则相反。
关键词(KeyWords)： 小麦;硫;亚细胞分布;复合胁迫
基金项目(Foundation): 国家自然科学基金项目(No.40871115;40671095)
作者(Author): 翁南燕;周东美;汪鹏;汪登俊;褚灵阳;
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