重金属积累对土壤酶活性的影响Effects of Accumulation of Heavy Metals in Soils on Enzyme Activities
高秀丽;邢维芹;冉永亮;周卫东;李立平;
摘要(Abstract):
研究了华北平原某铅冶炼厂附近农田33个土壤样品中重金属积累对土壤酶活性的影响。结果表明,样品中Pb和Cd全量的平均值分别为144和5.59mg·kg-1,DTPA态Pb和Cd含量平均值分别为54.1和0.964mg·kg-1,均超过了未污染农田潮土的正常范围,而Cu、Ni和Zn的有效性和全量与未污染土壤接近;土壤过氧化氢酶活性与DTPA态Pb和Cd含量、全Pb含量均呈显著的负线性关系(P<0.01);与磷酸酶和脲酶相比,土壤脱氢酶活性更易受到土壤中Pb和Cd积累的影响;随DTPA-Ni含量增加,土壤蛋白酶和碱性磷酸酶活性增加(P<0.1);土壤脲酶活性与重金属全量或有效态重金属含量无显著相关性(P>0.1)。以上结果说明,利用土壤过氧化氢酶和脱氢酶活性能够表征基本性质较为一致的土壤中重金属污染程度;与重金属全量相比,有效态重金属对土壤酶活性影响更大。
关键词(KeyWords): 酶活性;土壤;重金属;积累;农田;铅冶炼
基金项目(Foundation): 河南省教育厅自然科学研究计划项目(2011A610006)
作者(Author): 高秀丽;邢维芹;冉永亮;周卫东;李立平;
Email:
DOI:
参考文献(References):
- [1]Brookes P C.The use of microbial parameters in monitoring soil pollution by heavy metals[J].Biology and Fertility of Soils,1995,19(4):269-279
- [2]Gülser F,Erdogan E.The effects of heavy metal pollution on enzyme activities and basal soil respiration of roadside soils[J].Environmental Monitoring and Assessment,2008,145(1-3):127-133
- [3]Bastida F,Zsolnay A,Hernández T,et al.Past,present and future of soil quality indices:A biological perspective[J].Geoderma,2008,147(3-4):159-171
- [4]Lebrun J D,Trinsoutrot-Gattin I,Vinceslas-Akpa M,et al.Assessing impacts of copper on soil enzyme activities in regard to their natural spatio-temporal variation under long-term different land uses[J].Soil Biology and Biochemistry,2012,49:150-156
- [5]冉永亮,邢维芹,梁爽,等.华北平原地区某铅冶炼厂附近土壤重金属有效性研究[J].生态毒理学报,2010,5(4):592-598Ran Y L,Xing W Q,Liang S,et al.Heavy metal availability in soil near a lead smelter in the North China Plian[J].Asian Journal of Ecotoxicology,2010,5(4):592-598(in Chinese)
- [6]Amacher M C.Nickel,cadmium,and lead[M]//Methods of Soil Analysis,Part3.Chemical Methods.Madison,Wisconsin,USA:Soil Science Society of America Inc,1996:739-768
- [7]Tabatabai M A.Soil Enzymes[M]//Methods of Soil Analysis,Part2.Chemical and Microbiological Properties.Madison,Wisconsin,USA:Soil Science Society of America Inc,1982:903-948
- [8]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:123-339
- [9]贾琳,杨林生,欧阳竹,等.典型农业区农田土壤重金属潜在生态风险评价[J].农业环境科学学报,2009,28(11):2270-2276Jia L,Yang L S,Ouyang Z,et al.Assessment of the potential ecological risk of heavy metals in the farmland soils in Yucheng City,Shandong Province[J].Journal of Agro-Environment Science,2009,28(11):2270-2276(in Chinese)
- [10]谭长银,吴龙华,骆永明,等.典型潮土剖面主要性质和微量金属垂直分布特征[J].土壤学报,2009,46(5):817-824Tan C Y,Wu L H,Luo Y M,et al.Vertical distribution characteristics of selected soil properties and trace metals in typical calcareous fluvo-aquic soil profile[J].Acta Pedologica Sinica,2009,46(5):817-824(in Chinese)
- [11]李红伟,李立平,邢维芹.不同小尺度下潮土重金属有效性空间变异研究[J].土壤,2006,38(6):782-789Li H W,Li L P,Xing W Q.Spatial distribution of heavy metal availability in fluvo-aquic soil under different smallscales[J].Soils,2006,38(6):782-789(in Chinese)
- [12]Wang Y,Shi J,Wang H,et al.The influence of soil heavy metals pollution on soil microbial biomass,enzyme activity,and community composition near a copper smelter[J].Ecotoxicology and Environmental Safety,2007,67(1):75-81
- [13]胡学玉,孙宏发,陈德林.大冶矿区土壤重金属积累对土壤酶活性的影响[J].生态环境,2007,16(5):1421-1423Hu X Y,Sun H F,Chen D L.Influence of heavy metals pollution on the activities of soil enzyme in Daye mineral district in Hubei Province,China[J].Ecology and Environment,2007,16(5):1421-1423(in Chinese)
- [14]陈苏,孙丽娜,晁雷,等.基于土壤酶活性变化的铅污染土壤修复基准[J].生态环境学报,2010,19(7):1659-1662Chen S,Sun L N,Chao L,et al.Probabilistic remediation criterions of contaminated soil for lead derived from soil enzyme activities[J].Ecology and Environmental Sciences,2010,19(7):1659-1662(in Chinese)
- [15]洪春来,贾彦博,王润屹,等.铅污染对土壤重金属及酶活性的影响[J].中国农学通报,2008,24(12):304-307Hong C L,Jia Y B,Wang R Y,et al.The effect of soil microbe and enzymes of lead pollution[J].Chinese Agricultural Science Bulletin,2008,24(12):304-307(in Chinese)
- [16]Gao Y,Zhou P,Mao L,et al.Assessment of effects of heavy metals combined pollution on soil enzyme activities and microbial community structure:Modified ecological dose response model and PCR-RAPD[J].Environmental Earth Sciences,2010,60(3):603-612
- [17]Pan J,Yu L.Effects of Cd or/and Pb on soil enzyme activities and microbial community structure[J].Ecological Engineering,2011,37(11):1889-1894
- [18]Xie W,Zhou J,Wang H,et al.Short-term effects of copper,cadmium and cypermethrin on dehydrogenase activity and microbial functional diversity in soils after longterm mineral or organic fertilization[J].Agriculture Ecosystems and Environment,2009,129(4):450-456
- [19]Kandeler E,Tscherko D,Bruce K D,et al.Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil[J].Biology and Fertility of Soils,2000,32(5):390-400
- [20]Majer B J,Tscherko D,Paschke A,et al.Effects of heavy metal contamination of soils on micronucleus induction in tradescantia and on microbial enzyme activities:A comparative investigation[J].Mutation Research,2002,515(1-2):111-124
- [21]Mikanova O.Effects of heavy metals on some soil biological parameters[J].Journal of Geochemical Exploration,2006,88(1-3):220-223
- [22]Brookes P C,McGrath S P,Klein D A,et al.Effects of heavy metals on microbial activity and biomass in field soils treated with sewage sludge.In:Environmental contamination[M].Edinburgh:CEP Consultants Ltd,1984:574-583
- [23]王涵,高树芳,罗丹,等.Cd Pb污染土壤中蛋白酶酸性磷酸酶脱氢酶活性的变化[J].农业环境科学学报,2010,29(3):500-505Wang H,Gao S F,Luo D,et al.Changes of protease,acid phosphomonoesterase and dehydrogenase activities in Cd-Pb polluted agricultural soils[J].Journal of Agro Environment Science,2010,29(3):500-505(in Chinese)
- [24]Lee S,Kim E,Hyun S,et al.Metal availability in heavy metal-contaminated open burning and open detonation soil:Assessment using soil enzymes,earthworms,and chemical extractions[J].Journal of Hazardous Materials,2009,170(1):382-388
- [25]王涵,王果,林清强,等.Cu Cd Pb Zn对酸性耕作土壤3种酶活性的影响[J].农业环境科学学报,2009,28(7):1427-1433Wang H,Wang G,Lin Q Q,et al.The effects of Cu,Cd,Pb and Zn on the activities of urease,protease and peroxidase in acid cultivated soil[J].Journal of Agro-Environment Science,2009,28(7):1427-1433(in Chinese)
- [26]王玉军,周东美,孙瑞娟,等.土壤中草甘膦与镉的交互作用对3种土壤酶活性的影响[J].生态毒理学报,2006,1(1):58-63Wang Y J,Zhou D M,Sun R J,et al.Effects of glyphosate and Cd interaction on the activities of several soil enzymes[J].Asian Journal of Ecotoxicology,2006,1(1):58-63(in Chinese)
- [27]土壤环境质量标准(GB15618-1995)[S].北京:中国标准出版社,1995