2,3,7,8-四氯二苯并对二噁英对大鼠卵巢颗粒细胞雌二醇和孕酮分泌的影响Effects of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Estradiol and Progestone Secretion of Ovarian Granulosa Cells in Rats in Vitro
许振成;许虹;张素坤;邢福祺;
摘要(Abstract)：
为研究2,3,7,8-四氯二苯并对二噁英(TCDD)对体外原代培养大鼠卵巢颗粒细胞活力和雌二醇(Estradiol,E2)、孕酮(Progestone,P)分泌的影响,探索TCDD对卵巢颗粒细胞的毒性作用阈值和程度,对未成熟SD大鼠卵巢颗粒细胞进行了原代培养,并设2组体外染毒实验:终浓度分别为0.1、1、10、100nmol·L-1TCDD染毒24h和10nmol·L-1TCDD染毒1、3、6、18、24h.染毒结束后采用MTT法检测细胞活力,RIA法检测收集培养液中的E2和P含量.结果表明,1、10、100nmol·L-1TCDD可显著抑制大鼠卵巢颗粒细胞活力和E2、P的分泌,与阴性对照、0.1%DMSO组、0.1nmol·L-1TCDD组比较差异均有显著性(p<0.05);10nmol·L-1TCDD染毒6、18、24h可明显抑制细胞活力和E2、P的分泌,与对照组和短期染毒组(1h、3h)差异均有显著性(p<0.05).痕量的TCDD可显著降低大鼠卵巢颗粒细胞活力并抑制细胞E2和P分泌,对大鼠卵巢颗粒细胞的毒性作用阈值可能为1nmol·L-1或更低.TCDD的生殖毒性作用可能与直接对卵巢颗粒细胞的毒性作用和抑制甾体激素的生物合成以及分泌有关.
关键词(KeyWords)： TCDD;颗粒细胞;细胞活力;雌二醇;孕酮
基金项目(Foundation): 公益性科研院所基本科研业务费专项资金(No.zx_200809_21)
作者(Author): 许振成;许虹;张素坤;邢福祺;
Email:
DOI:
参考文献(References)：
- Dasmahapatra A K,Wimpee B A,Trewin A L,Wimpee C F,Ghorai J K,Hutz R J.2000.Demonstration of2,3,7,8-tetrachlorodibenzo-p-dioxin attenuation of P450steroidogenicenzyme mRNAs in rat granulosa cell in vitro by competitivereverse transcriptase-polymerase chain reaction assay[J].Molecularand Cellular Endocrinology,164:5-18
- Guo Y L,Hsu P C,Hsu C C,Lambert G H.2000.Semenquality after prenatal exposure to polychlorinated biphenyls anddibenzofurans[J].Lancet,356:1240-1241
- Heimler I,Rawlins R G,Owen H,Hutz R J.1998.Dioxinperturbs,in a dose-and time-dependent fashion,steroid secretion,and induces apoptosis of human luteinized granulose cells[J].Endocrinology,139:4373-4379
- Ho H M,Ohshima K,Watanabe G,Taya K,Strawn E Y,HutzR J.2006.TCDD increases inhibin A production by humanlureinized granulosa cells in vitro[J].Journal of Reproductiveand Development,52:523-528
- Le T N,Johansson A.2001.Impact of chemical warfare withagent orange on women’s reproductive lives in Vietnam:a pilotstudy[J].Reproductive Health Matters,9:156-164
- Li B,Liu H Y,Dai L J,Lu J C,Yang Z M,Huang L.2006.The early embryo loss caused by2,3,7,8-tetrachlorodibenzo-p-dioxin may be related to the accumulation of this compound inthe uterus[J].Reproductive Toxicology,21:301-306
- Morán F M,Enan E,Vandervoort C A,Stewart D R,Conley AJ,Overstreet J W,Lasley B L.1997.2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)effects on steroidogenesis of human luteinizedgranulosa cells in vitro[J].Society for the Study of Reproduction,56:99
- Morán F M,Lohstroh P,VandeVoort C A,Chen J,Overstreet JW,Conley A J,Lasley B L.2003.Exogenous steroid substratemodifies the effect of2,3,7,8-tetrachlorodibenzo-p-dioxin onestradiol production of human luteinized granulosa cells in vitro[J].Biology of Reproduction,68:244-251
- Wu Q,Ohsako S,Baba T,Miyamoto K,Tohyama C.2002.Effects of2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)onpreimplantation mouse embryos[J].Toxicology,174:119-129
- Zhao L J,Wang J,Liu Y H,Wang X J,Tang N J.2007.Effect of2,3,7,8-tetrachlorodibenzo-p-dioxin on the secretion ofovarian granulose cell in rats[J].Chinese Journal of IndustrialMedicine,20:45-46(in Chinese)
- 赵力军,王静,刘英华,王晓军,汤乃军.2007.二噁英对大鼠卵巢颗粒细胞分泌功能的影响[J].中国工业医学杂志,20:45-46