纳米水滑石与Hela细胞的生物相容性研究Study on Biocompatibility of Nano-Layered Double Hydroxides with Hela Cells
刘丹丹;付聪;李岩;袭著革;杨旭;
摘要(Abstract)：
为了探讨纳米水滑石与Hela细胞的生物相容性,采用单细胞凝胶电泳法检测了纳米水滑石对Hela细胞DNA的损伤,并采用H2DCF-DA荧光法检测了纳米水滑石对Hela细胞活性氧簇的影响.结果发现,随着纳米水滑石浓度的升高(0~800μg·mL-1),Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量均呈逐渐升高趋势;较低浓度(50、100μg·mL-1)纳米水滑石处理后,Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组无显著性差异(p>0.05),而较高浓度(200、400、800μg·mL-1)纳米水滑石处理后,Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组差异显著(p<0.05,p<0.01).以上结果表明,较高浓度(≥200μg·mL-1)的纳米水滑石可对Hela细胞DNA产生损伤,而较低浓度(≤100μg·mL-1)的纳米水滑石则具有较好的生物相容性,有可能作为载体应用于医药领域.
关键词(KeyWords)： 纳米水滑石;生物相容性;DNA损伤;Hela细胞
基金项目(Foundation): 国家高技术研究发展规划(863)项目(No. 2006AA03Z330)
作者(Author): 刘丹丹;付聪;李岩;袭著革;杨旭;
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