波尔多液对土壤过氧化氢酶活性与构象的影响Effects of Bordeaux Mixture on Soil Catalase Activity and Conformation Characteristics
傅丽君;钟开新;李华亮;
摘要(Abstract)：
为初步探讨波尔多液对土壤过氧化氢酶的作用机理及变化规律,采用人工模拟和荧光光谱法研究了波尔多液对土壤过氧化氢酶活性特征及构象的影响.结果表明:1)不同浓度波尔多液对土壤过氧化氢酶活性总体呈现低浓度激活、高浓度抑制作用.不同配制方式波尔多液对土壤过氧化氢酶活性抑制效应顺序为多倍量式>倍量式>半量式>等量式.浓度为200mg·kg-1左右的等量式波尔多液处理对土壤过氧化氢酶活性的影响最小.2)24h培养时间下,随波尔多液浓度的增加,酶促反应米氏常数Km和最大反应速率Vmax均呈先减小后增大趋势.统计分析表明,Km和Vmax均与波尔多液浓度呈显著正相关(p<0.01),土壤过氧化氢酶Km值可表征波尔多液对土壤过氧化氢酶活性的影响程度,其作用机理为反竞争性抑制类型.3)波尔多液对离体过氧化氢酶的内源荧光有较强的猝灭作用,Cu(Ⅱ)和离体过氧化氢酶的结合常数K及结合位点数n分别为69.1831mol·L-1和0.6878.
关键词(KeyWords)： 过氧化氢酶;波尔多液;动力学参数;酶活性;荧光光谱
基金项目(Foundation): 福建省科技厅项目(No.JK2009030);; 福建省高校服务海西重点项目(No.2008HX02;No.2008HX05);; 莆田市科技项目(No.2008N10(2))
作者(Author): 傅丽君;钟开新;李华亮;
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