波尔多液对土壤过氧化氢酶活性与构象的影响Effects of Bordeaux Mixture on Soil Catalase Activity and Conformation Characteristics
傅丽君;钟开新;李华亮;
摘要(Abstract)：
为初步探讨波尔多液对土壤过氧化氢酶的作用机理及变化规律,采用人工模拟和荧光光谱法研究了波尔多液对土壤过氧化氢酶活性特征及构象的影响.结果表明:1)不同浓度波尔多液对土壤过氧化氢酶活性总体呈现低浓度激活、高浓度抑制作用.不同配制方式波尔多液对土壤过氧化氢酶活性抑制效应顺序为多倍量式>倍量式>半量式>等量式.浓度为200mg·kg-1左右的等量式波尔多液处理对土壤过氧化氢酶活性的影响最小.2)24h培养时间下,随波尔多液浓度的增加,酶促反应米氏常数Km和最大反应速率Vmax均呈先减小后增大趋势.统计分析表明,Km和Vmax均与波尔多液浓度呈显著正相关(p<0.01),土壤过氧化氢酶Km值可表征波尔多液对土壤过氧化氢酶活性的影响程度,其作用机理为反竞争性抑制类型.3)波尔多液对离体过氧化氢酶的内源荧光有较强的猝灭作用,Cu(Ⅱ)和离体过氧化氢酶的结合常数K及结合位点数n分别为69.1831mol·L-1和0.6878.
关键词(KeyWords)： 过氧化氢酶;波尔多液;动力学参数;酶活性;荧光光谱
基金项目(Foundation): 福建省科技厅项目(No.JK2009030);; 福建省高校服务海西重点项目(No.2008HX02;No.2008HX05);; 莆田市科技项目(No.2008N10(2))
作者(Author): 傅丽君;钟开新;李华亮;
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参考文献(References)：
- Aliyev S A,Gadjiyev D A,Mikailov F D.1984.Kinetic andthermodynamic characteristics of enzyme-invertase and urease inAzerbaijan soils[J].JIOYBOB(,11):55-66
- Dalal R C.1985.Distribution,salinity,kinetic and thermodynamiccharacteristics of urease activity in a vertisol profile[J].Australian Journal of Soil Research,23(4):49-60
- Fan H,Yuan Y J.2006.Effect of Ce4+on conformation of catalase[J].Journal of the Chinese Rare Earth Society,24(6):734-738(in Chinese)
- Feng X Z,Bai C L,Lin Z,Wang N X,Wang C.1998.Theinteraction between acridine orange and bovine serum albumin[J].Chinese Journal of Analytieal Chemistry,26(2):154-157(in Chinese)
- Fu L J,Li H L,Zhong K X.2009.Effects of fenvalerate onsoil catalase activity[J].Asian Journal of Ecotoxicology,4(2):265-270(in Chinese)
- Fu L J,Yang W J,Wei Y Y.2009.Effects of copper pollutionon the activity of soil invertase and urease in loquat orchards[J].Chinese Journal of Geochemistry,28(1):76-80
- Fu L J,Zhao S X,Wang H,Yang W J.2005.Effects of fourpesticides on catalase activity in soil and soil respiration[J].Journal of Fujian Agricultural and Forestry University(NaturalScience Edition),34(4):441-445(in Chinese)
- Gao Z M.1983.Encyclopedia of China,Environmental ScienceVolume[M].Beijing:China Encyclopedia Publishing Company(in Chinese)
- He W X,Zhu M E.1997.Study on relation between urease andfertility of soils in Shaanxi II.Soil urease kinetic characteristics[J].Acta Pedologica Sinica,34(1):42-52(in Chinese)
- Hu X J,Yang W C,Liu W P.2004.Influence of atrazine oncatalase activity in soil[J].Environmental Pollution&Control,26(2):87-89(in Chinese)
- Lima J S.1994.Copper balances in cocoa agrarian ecosystems:Effects of differential use of cupric fungicides[J].AgricultureEcosystems and Environment,48(1):19-25
- Ministry of Environmental Protection of the People’s Republic ofChina.1995.GB15618-1995Environmental Quality Standard forSoils[S].Beijing:Standards Press of China(in Chinese)
- Roberts A H,Cameron K C,Bolan N L.1996.Contaminationof the Soil and Environment in New Zealand[M].TheNetherlands:Kluwer Academic Publisher
- Yang C L,Sun T H,He W X,Chen S.2007a.Effects of Hgon soil enzyme activity[J].Chinese Journal of Applied Ecology,18(3):620-624(in Chinese)
- Yang C L,Sun T H,He W X,Song X Y.2007b.Effect oftemperature on kinetic of soil urease inhibited by Hg[J].Environmental Science,28(2):278-282(in Chinese)
- Yang C L,Sun T H,He W X,Zhou Q X,Chen S.2007.Single and joint effects of pesticides and mercury on soil urease[J].Journal of Environmental Sciences,19(2):210-216
- Yang W C,Wang Q Q,Liu W P.2000.Adsorption ofherbicide atrazine on soils[J].Chinese Journal of EnvironmentalScience,21(4):94-97(in Chinese)
- Yi P G,Yu Q S,Hu X G,Shang Z C,Mei M H,Lin R S.2000.Activity and conformation of catalase in water-ethanolmixtures[J].Acta Chimica Sinica,58(6):652-655(in Chinese)
- Zhao Y H,Yuan X Y,Guo D S.2004.Effect of Cu2+and Cd2+on activity and fluorescence spectrum of catalase[J].Journal ofAgro-Environmental Science,23(6):1086-1088(in Chinese)
- 范寰,元英进.2006.Ce4+对过氧化氢酶构象的影响[J].中国稀土学报,24(6):734-738
- 冯喜增,白春礼,林璋,王乃新,王琛.1998.吖啶橙与牛血清白蛋白的相互结合反应[J].分析化学,26(2):154-157
- 傅丽君,李华亮,钟开新.2009.杀灭菊酯对土壤过氧化氢酶活性的影响[J].生态毒理学报,4(2):265-270
- 傅丽君,赵士熙,王海,杨文金.2005.4种农药对土壤微生物呼吸及过氧化氢酶活性的影响[J].福建农林大学学报(自然科学版),34(4):441-445
- 高拯民.1983.中国百科全书环境科学卷[M].北京:中国大百科出版社
- 国家环境保护总局.1995.GB15618-1995土壤环境质量标准[S].北京:中国标准出版社
- 和文祥,朱铭莪.1997.陕西土壤脲酶活性与土壤肥力关系研究Ⅱ.土壤脲酶的动力学特征[J].土壤学报,34(1):42-52
- 胡晓捷,杨炜春,刘维屏.2004.莠去津对土壤过氧化氢酶活性的影响研究[J].环境污染与防冶,26(2):87-89
- 杨春璐,孙铁珩,和文祥,陈苏.2007a.汞对土壤酶活性的影响[J].应用生态学报,18(3):620-624
- 杨春璐,孙铁珩,和文祥,宋雪英.2007b.温度对汞抑制土壤脲酶动力学影响研究[J].环境科学,28(2):278-282
- 杨炜春,王琪全,刘维屏.2000.除草剂莠去津(atrazine)在土壤-水环境中的吸附及其机理[J].环境科学,21(4):94-97
- 易平贵,俞庆森,胡新根,商志才,梅明辉,林瑞森.2000.水-乙醇混合溶剂中过氧化氢酶活性与构象的研究[J].化学学报,58(6):652-655
- 赵艳红,袁小英,郭栋生.2004.Cu2+、Cd2+对过氧化氢酶活性的影响及其荧光光谱研究[J].农业环境科学学报,23(6):1086-1088