余苗苗;江敏;吴昊;吴丹;金若晨;孙世玉;姚丹;王旭娜;

• 1:上海海洋大学海洋生态与环境学院
• 2:上海市水产养殖工程技术研究中心
• 3:上海海洋大学水产与生命学院

摘要(Abstract)：

通过气质联用仪和液质联用仪的非靶向检测,结合主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)等方法,对日本沼虾肝胰腺进行代谢物分析,以研究在非致死剂量的亚硝酸盐或氨胁迫下,日本沼虾肝胰腺新陈代谢的变化。经亚硝酸盐(0.5 mg·L~(-1),以N计)胁迫2 d后,鉴定到具有显著差异的代谢化合物46个,涉及氨基酸代谢、脂肪酸代谢和甘油磷脂代谢等; p H=9.0时,氨(1.0 mg·L~(-1),以N计,即非离子氨氮为0.378 mg·L~(-1))胁迫后,鉴定出显著差异性代谢化合物52个,涉及三羧酸循环、氨基酸代谢和甘油磷脂等代谢通路。选择p H=9.0氨(1.0 mg·L~(-1))胁迫组对日本沼虾肝胰腺中的三羧酸循环进行定量验证实验,结果显示,日本沼虾肝胰腺中草酰乙酸含量与对照组相比在12 h显著升高,24 h和48 h后则与对照组无显著差异。α-酮戊二酸含量与对照组相比在12 h即有显著增加,24 h含量与对照组相比显著下降,48 h后与对照组相比无明显差异。推测p H=9.0时氨氮(1.0 mg·L~(-1))胁迫12 h即会对三羧酸循环产生影响。

关键词(KeyWords)： 亚硝酸盐;氨;日本沼虾;肝胰腺;三羧酸循环;代谢组学

Abstract:

Keywords:

基金项目(Foundation): 上海市产业技术体系建设项目(沪农科产字(2014)第5号);; 上海市教委重点学科建设项目(No.J50701);; 上海市高校知识服务平台项目(No.ZF1206)

作者(Author): 余苗苗;江敏;吴昊;吴丹;金若晨;孙世玉;姚丹;王旭娜;

Email:

DOI:

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