汪庆;罗义;崔玉晓;齐丽英;周烁磊;杨光;

• 1:河北工程大学能源与环境工程学院河北省水污染控制与水生态修复工程技术研究中心
• 2:环境污染过程与基准教育部重点实验室南开大学

摘要(Abstract)：

环境中广泛存在的抗生素和抗生素抗性基因会导致很严重的人类健康风险。在我们前期研究中发现,离子液体1-丁基-3-甲基咪唑六氟磷酸盐([BMIm][PF6])作为环境选择性压力可以促进抗生素抗性基因的水平转移。本研究以E.coli DH5α(RP4)和同属的E.coli HB101,以及E.coli DH5α(RP4)和跨属的Salmonella enterica之间的纯菌接合转移体系为考察对象,从mRNA基因表达调控水平角度阐明离子液体[BMIm][PF6](0.001~2.5 g·L-1)影响质粒RP4接合转移的机理。结果表明,离子液体[BMIm][PF6]通过抑制基因kor A,kor B和trb A的mRNA表达水平来提高接合和跨膜转运基因trb Bp和trf Ap的表达;增强水平转移基因tra F的mRNA表达水平,并通过增强负调控基因kil A和kil B的mRNA表达水平抑制质粒的垂直传递过程;通过抑制基因trb K的mRNA表达水平降低接合转移体系的排斥效果,从而促进质粒RP4的接合转移。该结果有助于为抗生素抗性基因在环境中水平转移扩散的机理研究提供理论依据。

关键词(KeyWords)： 离子液体;抗生素抗性基因;质粒RP4;接合转移;mRNA表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41473085);; 环境污染过程与基准教育部重点实验室开放基金(KL-PPEC-2016-1);; 河北省自然科学基金(No.D2017402109);; 河北省教育厅青年基金(No.QN2017035);; 邯郸市科技计划项目(1621212047)

作者(Author): 汪庆;罗义;崔玉晓;齐丽英;周烁磊;杨光;

Email:

DOI:

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