甲醛所致DNA-蛋白质交联修复的研究Study on the Repair of DNA-Protein Crosslinks Induced by Formaldehyde
彭光银;赵玮;鲁志松;刘英帅;杨旭;
摘要(Abstract)：
为了探讨机体对甲醛所致DNA-蛋白质交联的修复能力,采用KCl-SDS沉淀法检测甲醛染毒后不同时间段HepG2细胞和小鼠肝细胞中DNA-蛋白质交联的修复情况.实验结果表明,采用75μmol·L-1甲醛染毒HepG2细胞,在染毒18h和24h后,细胞内的DPC水平较染毒结束时发生了显著下降(p<0.01),且与空白对照组相比无显著差异;由3.0mg·m-3气态甲醛产生的DNA-蛋白质交联在12h内可以得到明显修复(p<0.01),并在24h内恢复到空白对照水平;经20mg·kg-1液态甲醛染毒后,18h时DPC含量与空白组相比有显著性升高(p<0.01),在24h时DPC含量与18h时相比有显著的下降(p<0.05),且与空白组相比无显著差异.以上结果显示:甲醛所致肝细胞DNA-蛋白质交联能够得到修复,且24h内能够修复完全.
关键词(KeyWords)： 甲醛;体内实验;体外实验;DNA-蛋白质交联;修复作用
基金项目(Foundation): 国家自然基金面上项目(No.30570799)
作者(Author): 彭光银;赵玮;鲁志松;刘英帅;杨旭;
Email:
DOI:
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