水体中石油污染土壤对鲫鱼及其肝脏抗氧化系统的毒性效应The Toxicity of Oil Polluted Soil in Aquatic Environment on Carassius auratus and Its Hepatic Antioxidant Defense System
王媛媛;周启星;
摘要(Abstract):
在室内模拟条件下,研究了水体中不同浓度石油污染土壤暴露20d对鲫鱼(Carassius auratus)幼体死亡率和肝脏抗氧化系统的影响.结果表明,鲫鱼死亡率随其暴露浓度的变化明显分为3个部分:低浓度(0.5~5.0g·L-1)摄食死亡,中等浓度(5.0~25.0g·L-1)吸收死亡,高浓度(25.0~50.0g·L-1)胁迫死亡.1.0g·L-1浓度组死亡率最高,死亡速率最快;50.0g·L-1浓度组在暴露后期死亡速率迅速升高.鲫鱼肝脏中谷胱甘肽硫转移酶(GST)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性可被显著诱导,具体表现为:在所设浓度范围内,幼体鲫鱼肝脏GST活性均受到显著激活,0.5g·L-1浓度下,GST活性被最大程度诱导,达到对照组的606%;SOD活性先升高后降低,10.0g·L-1时酶活性最强,50.0g·L-1浓度下活性被显著抑制;CAT活性于0.5g·L-1就被显著诱导,2.5g·L-1浓度是对照组的4.86倍.可以认为,鲫鱼肝脏SOD和CAT,尤其GST活性对水体中石油污染土壤较敏感,均可作为水生生态系统中石油污染存在的早期检测指标.
关键词(KeyWords): 鲫鱼;石油污染土壤;生态毒性;抗氧化系统;水环境
基金项目(Foundation): 国家自然科学基金面上项目(No.20777040);; 国家高技术研究发展计划(863)重点项目(No.2007AA061201)
作者(Author): 王媛媛;周启星;
Email:
DOI:
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