易先亮;孙桦奇;张可可;张明乾;

• 1:大连理工大学食品与环境学院

摘要(Abstract)：

在我国近岸海域环境中,三苯基锡分布十分广泛。本研究以假微型海链藻(Thalassiosira pseudonana)为目标物种,研究了4个不同温度条件下(10℃、15℃、25℃与30℃)三苯基锡暴露对其细胞生长以及光合作用参数(Fv/Fm与ФII)的影响。结果表明,假微型海链藻的细胞生长速率在高温与低温条件下均显著下降,而光合作用参数仅在30℃条件下受到显著影响。在暴露温度为10℃、15℃、25℃与30℃时,三苯基锡(triphenyltin,TPT)对假微型海链藻的生长抑制IC50值分别为1.81、1.69、1.09与0.73μg·L-1。TPT对假微型海链藻光合作用抑制的IC50随着温度的升高呈降低趋势。Two-way ANOVA分析结果显示,温度与三苯基锡的相互作用显著影响假微型海链藻的光合作用。上述研究结果可以为了解不同温度下三苯基锡对海洋微藻的毒性效应提供科学依据。

关键词(KeyWords)： 三苯基锡;假微型海链藻;生长抑制;光合作用

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金(No.41606131);; 大连理工大学基本科研业务费(DUT15RC(3)069);; 工业生态与环境工程教育部重点实验室(KLIEEE-15-09)

作者(Author): 易先亮;孙桦奇;张可可;张明乾;

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DOI:

参考文献(References)：

• [1]Yi X,Leung K M Y,Lam M H W,et al.Review of measured concentrations of triphenyltin compounds in marine ecosystems and meta-analysis of their risks to humans and the environment[J].Chemosphere,2012,89(9):1015-1025
• [2]Ho K K Y,Leung K M Y.Organotin contamination in seafood and its implication for human health risk in Hong Kong[J].Marine Pollution Bulletin,2014,85(2):634-640
• [3]Armbrust E V.The life of diatoms in the world’s oceans[J].Nature,2009,459:185-192
• [4]Armbrust E V,Berges J A,Bowler C,et al.The genome of the diatom Thalassiosira pseudonana:Ecology,evolution,and metabolism[J].Science,2004,306(5693):79-86
• [5]Yi A X,Leung P T Y,Leung K M Y.Photosynthetic and molecular responses of the marine diatom Thalassiosira pseudonana to triphenyltin exposure[J].Aquatic Toxicology,2014,154:48-57
• [6]钱振明,邢荣莲,吴春雪,等.温度对8种底栖硅藻生长及其理化成分的影响[J].烟台大学学报,2009,22(1):30-34Qian Z,Xing R,Wu C,et al.Effect of temperature on growth and physiological biochemical compositions of eight benthic diatoms[J].Journal of Yantai University,2009,22(1):30-34(in Chinese)
• [7]刘杨平,黄迎春,王鹤立.浅谈环境因子对硅藻生长的影响[J].科技信息,2009(33):725-726Liu Y,Huang Y,Wang H.A brief discussion on the effect of environmental factors on the growth of diatom[J].Sciene and Technology Information,2009(33):725-726(in Chinese)
• [8]梁英,陈书秀,田传远,等.温度对三角褐指藻叶绿素荧光特性及生长的影响[J].中国海洋大学学报:自然科学版,2008,38(3):377-383Liang Y,Chen S,Tian C,et al.Effects of temperature on the chlorophyll fluorescence parameters and growth of Phaeodactylum tricornutum[J].Periodical of Ocean University of China,2008,38(3):377-383(in Chinese)
• [9]Wong S W Y.Ecotoxicological effects of selected engineered nano-materials to aquatic organisms in relation to their physicochemical characteristics[D].Hong Kong:The University of Hong Kong Press,2011:133-171
• [10]Yi X,Han J,Lee J-S,et al.Ecotoxicity of triphenyltin on the marine copepod Tigriopus japonicus at various biological organisations:From molecular to population-level effects[J].Ecotoxicology,2014,23(7):1314-1325
• [11]Yi X,Han J,Lee J-S,et al.Toxicity of triphenyltin chloride to the rotifer Brachionus koreanus across different levels of biological organization[J].Environmental Toxicology,2016,31(1):13-23
• [12]Li A J,Leung P T Y,Bao V W W,et al.Temperature-dependent toxicities of four common chemical pollutants to the marine medaka fish,copepod and rotifer[J].Ecotoxicology,2014,23(8):1564-1573
• [13]Bao V W W,Leng K M Y,Qiu J W,et al.Acute toxicities of five commonly used antifouling booster biocides to selected subtropical and cosmopolitan marine species[J].Marine Pollution Bulletin,2011,62(5):1147-1151
• [14]Lau E T C,Yung M M N,Karraker N E,et al.Is an assessment factor of 10 appropriate to account for the varia-tion in chemical toxicity to freshwater ectotherms under different thermal conditions[J].Environmental Science and Pollution Research,2014,21:95-104
• [15]Osterauer R,K9hler H R.Temperature-dependent effects of the pesticides thiacloprid and diazinon on the embryonic development of zebrafish(Danio rerio)[J].Aquatic Toxicology,2008,86(4):485-494
• [16]Deane E E,Woo N Y S.Cloning and characterization of the hsp70 multigene family from silver sea bream:Modulated gene expression between warm and cold temperature acclimation[J].Biochemical and Biophysical Research Communications,2005,330:776-783
• [17]Heugens E H W,Hendriks A J,Dekker T,et al.A review of the effects of multiple stressors on aquatic organisms and analysis of uncertainty factors for use in risk assessment[J].Critical Review in Toxicology,2001,31:247-284
• [18]Raisuddin S,Kwok K W H,Leung K M Y,et al.The copepod Tigriopus:A promising marine model organism for ecotoxicology and environmental genomics[J].Aquatic Toxicology,2007,83(3):161-173