王雅娟;马清萍;于洋;宋春磊;程逸飞;史珺怡;林志芬;

• 1:同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室
• 2:上海市化学品分析风险评估与控制重点实验室
• 3:上海污染控制与生态安全研究院
• 4:上海海洋大学海洋生态与环境学院

摘要(Abstract)：

抗生素的滥用使细菌耐药性问题日益突出,给许多疾病的预防与控制增加了难度。基因突变和质粒接合转移是细菌获得抗生素抗性基因的主要方式,许多研究围绕抗性基因来展开,但是关于群体感应对于抗性基因产生和传播的影响鲜有报道。本文以大肠杆菌(Escherichia coli)为模式生物,群体感应信号分子N-(β-酮己酰)-L-高丝氨酸内酯(3-oxo-C6-HSL,C6)和3种磺胺类抗生素(磺胺嘧啶、磺胺甲恶唑、磺胺氯哒嗪)为研究对象,测定了其对大肠杆菌生长效应、突变效应及接合转移效应的影响。结果表明:C6不影响磺胺对大肠杆菌的生长抑制率,但能够削弱磺胺对大肠杆菌突变的促进作用,并且能增强磺胺对大肠杆菌R388质粒接合转移的抑制作用。本文为从群体感应角度研究大肠杆菌耐药性的产生与传播提供新思路。

关键词(KeyWords)： 群体感应信号分子;磺胺;大肠杆菌;突变;接合转移

Abstract:

Keywords:

基金项目(Foundation): 同济大学污染控制与资源化研究国家重点实验室自主研究(重点)项目(PCRRK16007);; 国家自然科学面上基金(21577105,21777123);; 水体污染控制与治理科技重大专项(2018ZX07109-1);; 上海市科学技术委员会科研计划课题(14DZ2261100,17DZ1200103);; 环境化学与生态毒理学国家重点实验室开放基金课题(KF2016-11);; 111工程

作者(Author): 王雅娟;马清萍;于洋;宋春磊;程逸飞;史珺怡;林志芬;

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