四溴联苯醚对小鼠的神经毒性2,2',4,4'-Tetrabromodiphenyl Ether-induced Neurotoxicity in Mice
庄娟;李梦秋;洪法水;胡卫成;张萌萌;周颖君;罗艳;张建梅;郑元林;
摘要(Abstract):
为探讨持久性有机污染物2,2’,4,4’-四溴联苯醚(BDE-47)对小鼠海马组织的神经毒性,将小鼠每日灌胃25或50 mg·kg-1剂量的BDE-47,6周后检测其记忆能力、海马组织病理学变化、蛋白激酶C(PKC)表达量和半胱氨酸天冬氨酸蛋白酶-3(caspase-3)活性。结果显示,BDE-47损伤了小鼠被动回避实验的记忆保持能力,导致海马安蒙角(CA1)区锥体细胞排列紊乱,神经元胞体体积变小,形态不规则。BDE-47对小鼠海马组织PKCα、β、γ和ζ的表达无影响,但显著上调了PKCδ和PKCλ的表达,促进了caspase-3活性。这些结果提示,BDE-47导致的海马神经毒性可能与PKCδ和PKCλ表达异常及caspase-3活性升高有关。本实验结果为进一步了解BDE-47神经毒作用机制提供了实验依据。
关键词(KeyWords): BDE-47;海马组织;神经毒性;PKC;caspase-3
基金项目(Foundation): 江苏高校品牌专业建设工程资助项目(PPZY2015A018);; 国家自然科学基金(NO:81571055,31671033,31600281);; 江苏省高校自然科学基金(NO:18KJB330001)
作者(Author): 庄娟;李梦秋;洪法水;胡卫成;张萌萌;周颖君;罗艳;张建梅;郑元林;
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