徐红梅;宣怡红;江云;任娜;丁运生;吕丹;张毅帆;李小雪;周剑;许凯兵;

• 1:合肥工业大学医学工程学院 制药工程系
• 2:合肥工业大学安徽省先进功能材料与设备重点实验室
• 3:合肥工业大学化学工程学院

摘要(Abstract)：

研究离子液体溴化1-十四烷基-3-甲基咪唑盐([C14mim]Br)对小鼠的肝脏毒性及其作用机制。实验设立3个剂量的染毒组(1/16 LD50、1/8 LD50、1/4 LD50)和1个空白对照组,考察昆明种小鼠染毒14 d后,[C14mim]Br对小鼠血清生化指标、肝脏抗氧化酶活性及脂质过氧化产物的影响,并观察肝脏组织的病理变化。与对照组相比,小鼠染毒后,血清中ALT、AST、DBIL和γ-GT明显升高;肝组织受到不同程度的损伤,HSI增大,蛋白质含量降低。当染毒剂量为1/4 LD50时,肝脏中SOD活性和GSHPx活性显著降低,MDA的含量则明显增加。实验结果表明,[C14mim]Br可损伤小鼠的肝脏功能,破坏机体的抗氧化防御系统,造成氧化损伤和脂质过氧化。

关键词(KeyWords)： 离子液体;溴化1-十四烷基-3-甲基咪唑盐;肝毒性;小鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(50973025);; 合肥工业大学大学生创新创业实验(2012CXCY475)

作者(Author): 徐红梅;宣怡红;江云;任娜;丁运生;吕丹;张毅帆;李小雪;周剑;许凯兵;

Email:

参考文献(References)：

• [1]Docherty K M,Kulpa C F.Toxicity and antimicrobial activity of imidazolium and pyridinium ionic liquids[J].Green Chemistry,2005,7(4):185-189
• [2]Couling D J,Bernot R J,Docherty K M,et al.Assessing the factors responsible for ionic liquid toxicity to aquatic organisms via quantitative structure property relationship modeling[J].Green Chemistry,2006,8(1):82-90
• [3]Pernak J,Goc I,Mirska I.Anti-microbial activities of protic ionic liquids with lactate anion[J].Green Chemistry,2004,6(7):323-329
• [4]Jastorff B,Mlter K,Behrend P,et al.Progress in evaluation of risk potential of ionic liquids-basis for an eco-design of sustainable products[J].Green Chemistry,2005,7(5):362-372
• [5]Chen D C,Ye H Q,Wu H.A more deficient synthetic process of N-arylphthalimides in ionic liquid[bmim][BF4][J].Catalysis Communications,2007,8(10):1527-1530
• [6]Blaszczyk I,Gniewek A,Trzeciak A M.Monomeric triphenylphosphite palladacycles with N-imidazole ligands as catalysts of suzuki-miyaura and sonogashira reactions[J].Journal of Organometallic Chemistry,2011,696(23):3601-3604
• [7]Gonzalez E J,Calvar N,Gomez E,et al.Application of[Emim][ESO4]ionic liquid as solvent in the extraction of toluene from cycloalkanes:Study of liquid-liquid equilibria at T=298.15K[J].Fluid Phase Equilibria,2011,303(2):174-179
• [8]Zadegan S,Hosainalipour M,Rezaie H R,et al.Synthesis and biocompatibility evaluation of cellulose hydroxyapatite nanocomposite scaffold in 1-n-allyl-3-methylimidazolium chloride[J].Materials Science and Engineering:C,2011,31(5):954-961
• [9]Kulacki K J,Lamberti G A.Toxicity of imidazolium ionic liquids to freshwater algae[J].Green Chemistry,2008,10(1):104-110
• [10]Pretti C,Chiappe C,Pieraccin D,et al.Acute toxicity of ionic liquids to the zebrafish(Danio rerio)[J].Green Chemistry,2006,8(3):238-240
• [11]徐红梅,田芳,靳丹萍,等.咪唑型离子液体对小鼠的急性毒性[J].环境科学学报,2011,31(2):420-423Xu H M,Tian F,Jin D P,et al.Acute toxicity of imidazolium ionic liquid to mice[J].Acta Scientiae Circumstantiate,2011,31(2):420-423(in Chinese)
• [12]Frade R F M,Matias A,Branco L C,et al.Effect of ionic liquids on human colon carcinoma HT-29 and CaCo-2 cell lines[J].Green Chemistry,2007,9(8):873-877
• [13]Luo Y R,Wang S H,Li X Y,et al.Toxicity of ionic liquids on the growth,reproductive ability,and ATPase activity of earthworm[J].Ecotoxicology and Environmental Safety,2010,73(5):1046-1050
• [14]Li X Y,Zeng S H,Dong X Y,et al.Acute toxicity and responses of antioxidant systems to 1-methyl-3-octylimidazolium bromide at different developmental stages of goldfish[J].Ecotoxicology,2012,21(1):253-259
• [15]马剑敏,蔡林林,胡灵卫.溴化1-己基-3-甲基咪唑对斜生栅藻谷胱甘肽及其代谢酶的影响[J].水生生物学报,2009,33(4):696-701Ma J M,Cai L L,Hu L W,et al.Responses of glutahione cycle enzymes and gluta thione metabolism to 1-hexyl-3-methylimidazolium stress in Scenedesmus obliquus[J].Acta Hydroblogica Sinica,2009,33(4):696-701(in Chinese)
• [16]李效宇,皇培培,王春雨.早期胚胎发育期暴露1-辛基-3-甲基咪唑离子液体后对金鱼仔鱼的氧化损伤[J].生态毒理学报,2010,5(1):100-104Li X Y,Huang P P,Wang C Y.Oxidative stress of the ionic liquid 1-octyl-3-methylimidazolium bromide on the goldfish larva Carassius auratus hatching from the IL-treated embryos[J].Asian Journal of Ecotoxicology,2010,5(1):100-104(in Chinese)
• [17]Jeong S,Ha S H,Han S H,et al.Elucidation of molecular interactions between lipid membranes and ionic liquids using model cell membranes[J].Soft Matter,2012,8(20):5501-5506
• [18]Li X Y,Luo Y R,Yun M X,et al.Effects of 1-methyl-3-octylimidazolium bromide on the anti-oxidant system of earthworm[J].Chemosphere,2010,78(7):853-858
• [19]Kumar M,Trivedi N,Reddy C R K,et al.Toxic effects of imidazolium ionic liquids on the green seaweed Ulna lactuca:Oxidative stress and DNA damage[J].Chemical Research in Toxicology,2012,24(11):1882-1890
• [20]徐红梅,任娜,宣怡红,等.四氟硼酸化1-烷基-3-甲基咪唑离子液体对小鼠的急性毒性[J].环境科学学报,2013,33(1):298-303Xu H M,Ren N,Xuan Y H,et al.Acute oral toxicity of ionic liquid 1-alkyl-3-methylimidazolium tetrafluoborate to mice[J].Acta Scientiae Circumstantiae,2013,33(1):298-303(in Chinese)
• [21]Sheldon R.Catalytic reactions in ionic liquids[J].Chemical Communication,2001,1(23):2399-2407
• [22]Sónia P M V,Ana M M G,Tnia S,et al.Designing ionic liquids:The chemical structure role in the toxicity[J].Ecotoxicology,2013,22(1):1-12
• [23]Gal N,Malferarri D,Kolusheva S,et al.Membrane interactions of ionic liquids:Possible determinants for biological activity and toxicity[J].Biochimica et Biophysica Acta,2012,1818(12):2967-2974
• [24]Al-Rejaie S S,Aleisa A M,Al-Yahya A A,et al.Progression of diethylnitrosamine-induced hepatic carcinogenesis in carnitine-depleted rats[J].World Journal of Gastroenterology,2009,15(11):1373-1380
• [25]Gravato C,Teles M,Oliveira M,et al.Oxidative stress,liver biotransformation and genotoxic effects induced by copper in Anguilla anguilla L.——The influence of preexposure to beta-naphthoflavone[J].Chemosphere,2006,65(10):1821-1830
• [26]Gutteridge J M C.Lipid peroxidation,oxygen radicals,cell damage,and antioxidant therapy[J].Lancet,1984,6(23):1396-1397
• [27]Lesgards J F,Gauthier C,Iovanna J,et al.Effect of reactive oxygen and carbonyl species on crucial cellular antioxidant enzymes[J].Chemico-Biological Interactions,2011,190(1):28-34
• [28]周娜磊,李玥,高媛,等.大鼠肝脏过氧化氢酶的分离纯化及性质[J].氨基酸和生物资源,2009,31(2):56-68Zhou N L,Li Y,Gao Y,et al.Purification and some properties of catalase from rat liver[J].Amino Acids&Biotic Resources,2009,31(2):56-68(in Chinese)
• [29]徐冬梅,李婵丹,王艳花.全氟辛酸(PFOA)对蚯蚓的毒性作用[J].生态毒理学报,2012,7(5):532-536Xu D M,Li C D,Wang Y H.Toxicity of perfluorooctanoic acid to earthworms(Eisenia foetida)[J].Asian Journal of Ecotoxicology,2012,7(5):532-536(in Chinese)
• [30]侯东颖,冯佳,谢树莲.纳米二氧化钛胁迫对普生轮藻的毒性效应[J].环境科学学报,2012,32(6):1481-1486Hou D Y,Feng J,Xie S L.Toxic effects of nanoparticle TiO2stress on Chara vulgaris L[J].Acta Scientiae Circumstantiae,2012,32(6):1481-1486(in Chinese)
• [31]董玉瑛,杨璋梅,王晶,等.低剂量甲基毒死蜱对鲫鱼肝脏主要酶活性的影响[J].武汉大学学报:理学版,2012,58(4):322-326Dong Y Y,Yang Z M,Wang J,et al.Effects of methyl chlorpyrifosat low dose on major enzymatic activity in crucian's liver[J].Journal of Wuhan University:Natural Science Edition,2012,58(4):322-326(in Chinese)
• [32]Miao Y,Li S M,Li X Y,et al.Acute effects of 1-octyl-3-methylimidazolium bromide ionic liquid on the antioxidant enzyme system of mouse liver[J].Ecotoxicology and Environmental Safety,2008,71(3):903-908
• [32]Zhang J E,Yu J Y,Ouyang Y,et al.Responses of earthworm to aluminum toxicity in latosol[J].Environmental Science and Pollution Research,2013,20(2):1135-1141
• [34]Mallikarjuna K,Nishanth K,Reddy K S.Hepatic glutathione mediated antioxidant system in ethanol treated rats:Decline with age[J].Pathophysiology,2007,14(1):17-21
• [35]Xiang J,Zhu W X,Li Z X,et al.Effect of juice and fermented vinegar from Hovenia dulcispeduncles on chronically alcohol-induced liver damage in mice[J].Food Function,2012,3(6):628-634
• [36]汪美贞,贾秀英,董爱华,等.镉长期暴露对黑斑蛙的氧化胁迫和抗氧化能力的影响[J].生态学报,2006,26(6):1718-1724Wang M Z,Jia X Y,Dong A H,et al.Effects of cadmiumon oxidative stress and antioxidative ability in the frog Rana nigromaculata under a long-term exposure[J].Acta Ecologica Sinica,2006,26(6):1718-1724(in Chinese)
• [37]王莉,郭靖,韩冰,等.邻苯二甲酸二乙基己酯(DEHP)对小鼠的氧化损伤及黄豆异黄酮对其保护作用的研究[J].生态毒理学报,2012,7(3):268-276Wang L,Guo J,Han B,et al.Oxidative damage induced by di-(2-ethylhexyl)phthalate and protective effects of soybean isoflavones in mice[J].Asian Journal of Ecotoxicology,2012,7(3):268-276(in Chinese)
• [38]Lv Y J,Liu Z J,Tian Y J,et al.Effect on morphology,oxidative stress and energy metabolism enzymes in the testes of mice after a 13-week oral administration of melamine and cyanuric acid combination[J].Regulatory Toxicology and Pharmacology,2013,65(2):183-188