零价纳米铁对大肠杆菌的毒性效应Toxicity Effects of Nano-Fe~0 on Escherichia coli
王学;李勇超;李铁龙;金朝晖;
摘要(Abstract)：
以大肠杆菌为研究对象,通过检测尺寸为20nm的零价纳米铁暴露下大肠杆菌形貌、生长曲线和细胞内酶活性的变化,研究了零价纳米铁对大肠杆菌的毒性效应,并探讨了其可能的毒性机制。用透射电镜(TEM)观察零价纳米铁与大肠杆菌(JM109)接触后细胞的形态变化;用0、112、560和1120mg·L-1的零价纳米铁染毒大肠杆菌细胞,测定大肠杆菌的生长曲线变化;并测定零价纳米铁染毒24h后细胞培养液上清中乳酸脱氢酶(LDH)活性、细胞内超氧化物歧化酶(SOD)活性、丙二醛(MDA)的含量,同时观察加入抗氧化剂N-乙酰半胱氨酸(NAC)后细胞的生长变化。结果表明,零价纳米铁能够破坏细胞完整性,造成细胞损伤;抑制大肠杆菌的细胞生长,缩短大肠杆菌的对数期,延长稳定期;零价纳米铁浓度越高,大肠杆菌的稳定期越长。零价纳米铁还可导致细胞培养上清液中LDH活性显著升高,细胞内SOD活性显著下降,MDA含量显著升高,且MDA含量变化与零价纳米铁浓度存在剂量-效应关系。加入抗氧化剂NAC后,加NAC的实验组细胞数大于没加NAC的实验组。以上结果表明零价纳米铁的毒性机制为氧化损伤。
关键词(KeyWords)： 零价纳米铁;大肠杆菌;生长曲线;酶活性;氧化损伤
基金项目(Foundation): 国家青年科学基金项目(20907023);; 国家自然科学基金项目(40971254)
作者(Author): 王学;李勇超;李铁龙;金朝晖;
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