王学;李勇超;李铁龙;金朝晖;

• 1:南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室
• 2:天津市城市生态环境修复与污染防治重点实验室

摘要(Abstract)：

以大肠杆菌为研究对象,通过检测尺寸为20nm的零价纳米铁暴露下大肠杆菌形貌、生长曲线和细胞内酶活性的变化,研究了零价纳米铁对大肠杆菌的毒性效应,并探讨了其可能的毒性机制。用透射电镜(TEM)观察零价纳米铁与大肠杆菌(JM109)接触后细胞的形态变化;用0、112、560和1120mg·L-1的零价纳米铁染毒大肠杆菌细胞,测定大肠杆菌的生长曲线变化;并测定零价纳米铁染毒24h后细胞培养液上清中乳酸脱氢酶(LDH)活性、细胞内超氧化物歧化酶(SOD)活性、丙二醛(MDA)的含量,同时观察加入抗氧化剂N-乙酰半胱氨酸(NAC)后细胞的生长变化。结果表明,零价纳米铁能够破坏细胞完整性,造成细胞损伤;抑制大肠杆菌的细胞生长,缩短大肠杆菌的对数期,延长稳定期;零价纳米铁浓度越高,大肠杆菌的稳定期越长。零价纳米铁还可导致细胞培养上清液中LDH活性显著升高,细胞内SOD活性显著下降,MDA含量显著升高,且MDA含量变化与零价纳米铁浓度存在剂量-效应关系。加入抗氧化剂NAC后,加NAC的实验组细胞数大于没加NAC的实验组。以上结果表明零价纳米铁的毒性机制为氧化损伤。

关键词(KeyWords)： 零价纳米铁;大肠杆菌;生长曲线;酶活性;氧化损伤

Abstract:

Keywords:

基金项目(Foundation): 国家青年科学基金项目(20907023);; 国家自然科学基金项目(40971254)

作者(Author): 王学;李勇超;李铁龙;金朝晖;

Email:

DOI:

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