纳米氧化铜(CuO NPs)对紫花苜蓿幼苗根系活性氧(ROS)、抗氧化酶活性和根系活力的影响Effects of CuO NPs on ROS,Antioxidant Enzyme Activity and Root Activity of the Alfalfa Seedlings
刘振国;王天慧;王伟;
摘要(Abstract):
根系通常是植物直接受到外界环境物质(如重金属离子和纳米金属氧化物)毒害的主要器官。本研究以紫花苜蓿的幼苗为实验材料,探讨了CuO NPs胁迫对紫花苜蓿幼苗根系的活性氧(ROS)积累、抗氧化酶活性和根系活力的影响。研究结果表明:(1)不同浓度的CuO NPs对紫花苜蓿幼苗根系H_2O_2和O-2·含量具有显著影响。随CuO NPs浓度增大,紫花苜蓿幼苗根系中H_2O_2和O-2·整体上呈现先增大后减少的趋势,除了0.00125 mol·L~(-1)CuO NPs浓度下紫花苜蓿幼苗根系中H_2O_2含量比对照减少外,其他浓度下的H_2O_2和O-2·的含量都比对照有所增加,并且H_2O_2和O-2·的含量都是在0.0125 mol·L~(-1)CuO NPs浓度下达到最大值。(2)不同浓度的CuO NPs对紫花苜蓿幼苗根系抗氧化酶活性具有显著影响。随CuO NPs浓度增大,紫花苜蓿幼苗根系中SOD、POD、APX和CAT酶的活性都呈现先增大后减少的趋势,其中,SOD和POD、APX、CAT酶的活性分别是在0.00625、0.0125、0.0625 mol·L~(-1)CuO NPs处理下达到最大,表明这些抗氧化酶在清除根系中的活性氧方面表现出较强的协同性和补偿性。(3)不同浓度的CuO NPs对紫花苜蓿幼苗根系活力具有显著的影响。随着CuO NPs浓度的增加,紫花苜蓿幼苗根系活力逐步升高,这是一种生物适应性应激响应的体现。
关键词(KeyWords): CuO NPs;紫花苜蓿;活性氧;抗氧化酶;根系活力
基金项目(Foundation): 国家自然科学基金面上项目(21677093)
作者(Author): 刘振国;王天慧;王伟;
Email:
DOI:
参考文献(References):
- [1]白春礼.纳米科技及其发展前景[J].科学通报,2001,46(2):89-92Bai C L.Nano-technology and its developing prospect[J].Chinese Science Bulletin(Chinese Version),2001,46(2):89-92(in Chinese)
- [2]汪冰,丰伟悦,赵宇亮,等.纳米材料生物效应及其毒理学研究进展[J].中国科学B辑:化学,2005,35(1):1-10Wang B,Feng W Y,Zhao Y L,et al.Biological effects and progress in toxicology of nanomaterials[J].Science in China Series B:Chemistry,2005,35(1):1-10(in Chinese)
- [3]Masciangioli T,Zhang W X.Environmental technologies at the nanoscale[J].Environmental Science&Technology,2003,37(5):102A-108A
- [4]Klaine S J,Alvarez P J J,Batley G E,et al.Nanomaterials in the environment:Behavior,fate,bioavailability,and effects[J].Environmental Toxicology and Chemistry,2008,27:1825-1851
- [5]Saison C,Perreault F,Daigle J C,et al.Effect of coreshell copper oxide nanoparticles on cell culture morphology and photosynthesis(photosystem II energy distribution)in green alga,Chlamydomonas reinhardtii[J].Aquatic Toxicology,2010,96:109-114
- [6]Bhatt I,Tripathi B N.Interaction of engineered nanoparticles with various components of the environment and possible strategies for their risk assessment[J].Chemosphere,2011,82(3):308-317
- [7]Scown T M,van Aerle R,Tyler C R.Review:Do engineered nanoparticles pose a significant threat to the aquatic environment?[J]Critical Reviews in Toxicology,2010,40(7):653-670
- [8]Fahmy B,Cormier S A.Copper oxide nanoparticles induce oxidative stress and cytotoxicity in airway epithelial cells[J].Toxicology in Vitro,2009,23(7):1365-1371
- [9]Thit A,Selck H,Bjerregaard H F.Toxicity of Cu O nanoparticles and Cu ions to tight epithelial cells from Xenopuslaevis(A6):Effects on proliferation,cell cycle progression and cell death[J].Toxicology in Vitro,2013,27:1596-1601
- [10]Rodhe Y,Skoglund S,Wallinder I O,et al.Copperbased nanoparticles induce high toxicity in leukemic HL60 cells[J].Toxicology in Vitro,2015,29(7):1711-1719
- [11]陆其聪,方涛,戴和平,等.纳米氧化铜对酿酒酵母的细胞毒性机制研究[J].环境科学学报,2013,33(8):2341-2348Lu Q C,Fang T,Dai H P,et al.The cytotoxicity mechanism of Cu O nanoparticles to yeast Saccharomyces cerevisiae BY4741[J].Acta Scientiae Circumstantiae,2013,33(8):2341-2348(in Chinese)
- [12]Gunawan C,Teoh W Y,Marquis C P,et al.Cytotoxic origin of copper(II)oxide nanoparticles:Comparative studies with micron-sized particles,leachate,and metal salts[J].ACS Nano,2011,5(9):7214-7225
- [13]Shang E X,Li Y,Niu J F,et al.Effect of aqueous media on the copper-ion-mediated phototoxicity of Cu O nanoparticles toward green fluorescent protein-expressing Escherichia coli[J].Ecotoxicology and Environmental Safety,2015,122:238-244
- [14]Karlsson H L,Gustafsson J,Cronholm P,et al.Sizedependent toxicity of metal oxide particles—A comparison between nano-and micrometer size[J].Toxicology Letters,2009,188:112-118
- [15]Meng H,Chen Z,Xing G M,et al.Ultrahigh reactivity provokes nanotoxicity:Explanation of oral toxicity of nano-copper particles[J].Toxicology Letters,2007,175:102-110
- [16]Wang Z,Li J,Zhao J,et al.Toxicity and internalization of Cu O nanoparticles to prokaryotic alga Microcystis aeruginosa as affected by dissolved organic matter[J].Environmental Science&Technology,2011,45(14):6032-6040
- [17]Blinova I,Ivask A,Heinlaan M,et al.Ecotoxicity of nanoparticles of Cu O and Zn O in natural water[J].Environmental Pollution,2010,158(1):41-47
- [18]Aruoja V,Dubourgule H C,Kasemets K,et al.Toxicity of nanoparticles of Cu O,Zn O and Ti O2to microalgae Pseudokirchneriella subcapitata[J].Science of the Total Environment,2009,407:1461-1468
- [19]崔静,袁旭音,刘泉,等.环境水体中纳米氧化铜对金鱼藻的毒性效应研究[J].农业环境科学学报,2013,32(5):910-915Cui J,Yuan X Y,Liu Q,et al.Toxic effects of low concentration copper oxide nanoparticles on Ceratophyllum demersum in the aquatic environment[J].Journal of Agro-Environment Science,2013,32(5):910-915(in Chinese)
- [20]Perreault F,Oukarroum A,Pirastru L,et al.Evaluation of copper oxide nanoparticles toxicity using chlorophyll a fluorescence imaging in Lemna gibba[J].Journal of Botany,2010.doi:10.1155/2010/763142.
- [21]Gomes T,Pereira C G,Cardoso C,et al.Accumulation and toxicity of copper oxide nanoparticles in the digestive gland of Mytilus galloprovincialis[J].Aquatic Toxicology,2012,118-119:72-79
- [22]Siddiqui S,Goddard R H,Bielmyer-Fraser G K.Comparative effects of dissolved copper and copper oxide nanoparticle exposure to the sea anemone,Exaiptasia pallid[J].Aquatic Toxicology,2015,160:205-213
- [23]向垒,莫测辉,卢锡洪,等.纳米氧化铜对白菜种子发芽的毒害作用研究[J].农业环境科学学报,2011,30(9):1830-1835Xiang L,Mo C H,Lu X H,et al.Toxicity of copper oxide nanoparticles to the seed germination of Chinese cabbage[J].Journal of Agro-Environment Science,2011,30(9):1830-1835(in Chinese)
- [24]金盛杨,王玉军,汪鹏,等.纳米氧化铜对小麦根系生理生化行为的影响[J].土壤,2011,43(4):605-610Jin S Y,Wang Y J,Wang P,et al.Effects of Cu O nanoparticles on physiological and biochemical behaviors of wheat(Triticum aestivum L.)root[J].Soils,2011,43(4):605-610(in Chinese)
- [25]董宽虎.苜蓿产业化生产与加工利用[M].北京:金盾出版社,2002Dong K H.The industrialization of alfalfa production and processing application[M].Beijing:Jindun Publishing House,2002(in Chinese)
- [26]刘振国,王天慧,王伟.纳米氧化铜对紫花苜蓿种子发芽和幼苗生长的毒害作用[J].上海农业学报,2014,30(4):47-51Liu Z G,Wang T H,Wang W.Toxicity of nano-copper oxide on the seed germination and seedling growth of alfalfa[J].Acta Agriculturae Shanghai,2014,30(4):47-51(in Chinese)
- [27]魏志琴,陈志勇,秦蓉,等.Cu2+对拟南芥根的局部毒性及诱导DNA损伤和细胞死亡[J].植物学报,2013,48(3):303-312Wei Z Q,Chen Z Y,Qin R,et al.Cu2+induced local toxicity and DNA damage,cell death in roots of Arabidopsis thaliana[J].Chinese Bulletin of Botany,2013,48(3):303-312(in Chinese)
- [28]Banerjee B D,Seth V,Bhattacharya A,et al.Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers[J].Toxicology Letters,1999,107:33-47
- [29]Vale G,Mehennaoui K,Cambier S,et al.Manufactured nanoparticles in the aquatic environment-biochemical responses on freshwater organisms:A critical overview[J].Aquatic Toxicology,2016,170:162-174
- [30]He J,Ji Z X,Wang Q Z,et al.Effect of Cu and Pb pollution on the growth and antioxidant enzyme activity of Suaeda heteroptera[J].Ecological Engineering,2016,87:102-109
- [31]李忠光,宋玉泉,龚明.二甲酚橙法用于测定植物组织中的过氧化氢[J].云南师范大学学报,2007,27(3):50-54Li Z G,Song Y Q,Gong M.Xylenol orange method used for the measurement of hydrogen peroxide in plant tissue[J].Journal of Yunnan Normal University,2007,27(3):50-54(in Chinese)
- [32]李忠光,龚明.植物中超氧阴离子自由基测定方法的改进[J].云南植物研究,2005,27(2):211-216Li Z G,Gong M.Improvement of measurement method for superoxide anion radical in plant[J].Acta Botanica Yunnanica,2005,27(2):211-216(in Chinese)
- [33]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006Li H S.Principles and Techniques of Plant Physiology and Biochemistry[M].Beijing:Higher Education Press,2006(in Chinese)
- [34]Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidases in spinach chloroplasts[J].Plant and Cell Physiology,1981,22:867-880
- [35]张怡,路铁刚.植物中的活性氧研究概述[J].生物技术进展,2011,1(4):242-248Zhang Y,Lu T G.The research of reactive oxygen species(ROS)in plants[J].Current Biotechnology,2011,1(4):242-248(in Chinese)
- [36]Mittler R.Oxidative stress,antioxidants and stress tolerance[J].Trends in Plant Sciences,2002,7(9):405-410
- [37]林道辉,冀静,田小利,等.纳米材料的环境行为与生物毒性[J].科学通报,2009,54:3590-3604Lin D H,Ji J,Tian X L,et al.Environmental behavior and toxicity of engineered nanomaterials[J].Chinese Science Bulletin(Chinese Version),2009,54:3590-3604(in Chinese)
- [38]Nel A,Xia T,Mdler L,et al.Toxic potential of materials at the nanolevel[J].Science,2006,311(5761):622-627
- [39]Ahmad P,Jaleel C A,Salem M A,et al.Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress[J].Critical Reviews in Biotechnology,2010,30(3):161-175
- [40]Patra J,Lenka M,Panda B B.Tolerance and co-tolerance of the grass Chloris barbata Sw.to mercury,cadmium and zinc[J].New Phytologist,1994,128:165-171
- [41]胡金朝,施国新,丁秉中,等.Cd2+和Cr6+对慈姑(Sagittaria sagittifolia L.)的毒害效应[J].湖泊科学,2006,18(2):157-162Hu J C,Shi G X,Ding B Z,et al.The toxic effects of Cd2+and Cd6+on Sagittaria sagittifolia L.[J].Journal of Lake Sciences,2006,18(2):157-162(in Chinese)
- [42]Rizhsky L,Hallak-Herr E,van Breusegem F,et al.Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase[J].The Plant Journal,2002,32(3):329-342
- [43]施国新,杜开和,解凯彬,等.汞、镉污染对黑藻叶细胞伤害的超微结构研究[J].植物学报,2000,42(4):373-378Shi G X,Du K H,Xie K B.Ultrastructural study of leaf cells damaged from Hg2+and Cd2+pollution in Hydrilla verticillata[J].Acta Botanica Sinica,2000,42(4):373-378(in Chinese)
- [44]Griffitt R J,Weil R,Hyndman K A,et al.Exposure to copper nanoparticles causes gill injury and acute lethality in zebrafish(Danio rerio)[J].Environmental Science&Technology,2007,41(23):8178-8186