赵旭;叶幸;张燕;宋伟华;姜辉;瞿唯钢;

• 1:农业部农药检定所

摘要(Abstract)：

苏云金芽孢杆菌、球孢白僵菌以及棉铃虫核型多角体病毒是细菌类、真菌类和病毒类微生物农药的典型代表。为了明确微生物农药对家蚕的毒性影响,选取以上3种典型微生物农药,分别探究其对家蚕的毒性。结果显示:苏云金杆菌原药对家蚕LC50值为1.33×105CFU·m L-1;球孢白僵菌原药对家蚕LC50值为1.14×106CFU·m L-1;棉铃虫核型多角体病毒母药对家蚕LC50值大于1.00×108PIB·m L-1。与此同时,通过进一步试验,明确了以上3种微生物农药对家蚕结茧率、死笼率、全茧量、茧层量等关键指标的影响。结果显示:除了棉铃虫核型多角体病毒母药,高浓度苏云金杆菌原药、球孢白僵菌原药对家蚕的结茧率和死笼率均具有显著影响;3种微生物农药对家蚕产茧能力指标全茧量、茧层量以及蛹重均无显著性影响。

关键词(KeyWords)： 微生物农药;家蚕;毒性

Abstract:

Keywords:

基金项目(Foundation): 农业部以《农业部办公厅关于下达2016年农业行业标准制订和修订及农产品质量认证项目的通知》(农办质[2016]28号)下达的《微生物农药环境风险评价试验准则家蚕毒性试验》制定项目

作者(Author): 赵旭;叶幸;张燕;宋伟华;姜辉;瞿唯钢;

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参考文献(References)：

• [1]陈源,卜元卿,单正军.微生物农药研发进展及各国管理现状[J].农药,2012,51(2):83-89Chen Y,Bu Y Q,Shan Z J.Reviews on development and management of microbial pestcide in different countries[J].Agrochemicals,2012,51(2):83-89(in Chinese)
• [2]张化霜.微生物农药研究进展[J].农药科学与管理,2011,32(11):22-25Zhang H S.Research advance of microbial pesticides[J].Pesticides Science and Administration,2011,32(11):22-25(in Chinese)
• [3]段永兰,侯金丽,邢文会.我国微生物农药的研究与展望[J].安徽农业科学,2010,38(8):4135-4138Duan Y L,Hou J L,Xing W H.Research and prospect of microbial pesticide in China[J].Journal of Anhui Agri cultural Science,2010,38(8):4135-4138(in Chinese)
• [4]卜元卿,刘常宏,单正军.微生物农药环境安全性评价技术研究[M].北京:科学出版社,2015:25Bu Y Q,Liu C H,Shan Z J.Research on Technology in Risk Assessment of Microbial Pesticides[M].Beijing:Science Press,2015:25
• [5]Milner R J,Lim R P,Hunter D M.Risks to the aquatic ecosystem from the application of Metarhizium anisopliae for locust control in Australia[J].Pest Management Science,2002,58(6):718-723
• [6]Chatterjee H,Senapai S K.Bio-safety evaluation of some microbial insecticides against spiders and coccinellids[J].Journal of Biopesticides,2010,3(1):74-76
• [7]陈伟国,鲁兴萌.农药对家蚕的毒性和安全性评价研究[J].蚕业科学,2012,38(2):329-336Chen W G,Lu X M.An investigation on toxity and safety evaluation of pesticides to silkworm,Bombyx mori[J].Science of Sericulture,2012,38(2):329-336(in Chinese)
• [8]刘挺,黄可威.蚕病及其防治研究进展[J].蚕业科学,2000,26(S1):60-68Liu T,Huang K W.Recent advancements in silkworm diseases[J].Science of Sericulture,2000,26(S1):60-68(in Chinese)
• [9]金健康.蚕病诊断与防治普及系统[D].苏州:苏州大学,2007:1-69Jing J K.Popularization system for diagnosis and prevention of silkworm disease[D].Suzhou:Suzhou University,2007:1-69(in Chinese)
• [10]吴洪丽,孙波,叶建美,等.家蚕细菌病防治研究进展[J].北方蚕业,2014,35(4):1-3,8Wu H L,Sun B,Ye J M,et al.Research progress on prevention and treatment of bacterial diseases of silkworm[J].North Sericulture,2014,35(4):1-3,8(in Chinese)
• [11]吕鹏,潘晔,王鹏,等.家蚕抗核型多角体病毒的研究进展[J].科学通报,2015,26(14):1285-1297Lv P,Pan Y,Wang P,et al.Progress in understanding resistance of Bombyx mori to nucleopolyhedrovirus[J].Chinese Science Bulletin,2015,26(14):1285-1297(in Chinese)
• [12]关雄,蔡峻.我国苏云金杆菌研究60年[J].微生物学通报,2014,41(3):459-465Guan X,Cai J.Bacillus thuringiensis:Sixty years of research,development and commercial applications in China[J].Microbiology China,2014,41(3):459-465(in Chinese)
• [13]王利平,代林远,李鹏.苏云金芽孢杆菌研究进展[J].中国畜牧兽医,2011,38(9):224-227Wang L P,Dai L Y,Li P.Research advances on Bacillus thuringiensis[J].China Animal Husbandry and Veterinary Medicine,2011,38(9):224-227(in Chinese)
• [14]李国军,韦秉兴,韦绥概,等.Bt农药对“桂蚕一号”家蚕毒性的测定[J].广西蚕业,2008,45(4):15-19Li G J,Wei B X,Wei S G,et al.Measurement of toxicity of Bt preparation“Guican No.1”silkworm variety[J].Guangxi Sericulture,2008,45(4):15-19(in Chinese)
• [15]张燕,杨亚哲,袁善奎,等.几种生物农药对家蚕的急性毒性研究[J].农药科学与管理,2015,36(3):32-35Zhang Y,Yang Y Z,Yuan S K,et al.Study on the acute toxicity of several biological pesticides to silkworm[J].Pesticides Science and Administration,2015,36(3):32-35(in Chinese)
• [16]沈飞.三种典型微生物农药对家蚕、蜜蜂的安全性研究[D].南京:南京大学,2013:1-76Shen F.Safety assessment of three typical microbial pesticides on silkworm and honey bee[D].Nanjing:Nanjing University,2013:1-76(in Chinese)
• [17]陈源.微生物农药对鱼类安全性测试方法及影响研究[D].南京:南京信息工程大学,2013:1-119Chen Y.Study on safety assessment methods and effects of typical microbial pesticides on fish[D].Nanjing:Nanjing University of Information Science and Technology,2013:1-119(in Chinese)
• [18]余素红,曾明森,吴光远.球孢白僵菌的研究应用与展望[J].茶叶科学技术,2009,18(3):8-11Yu S H,Zeng M S,Wu G Y.Research and prospect of Beauveria bassiana[J].Tea Science and Technology,2009,18(3):8-11(in Chinese)
• [19]Huang L L,Cheng T,Xu P,et al.A genome-wide survey for host response of silkworm,Bombyx mori,during pathogen,Bacillus bombyseptieus,infection[J].Plo S one,2009,4(12):1-18
• [20]石美宁,朱方容.广西林用白僵菌生物农药对家蚕的致病性试验[J].广西农业科学,2010,10(1):1131-1133Shi M N,Zhu F R.Pathogellicity test of forest-using Beauveria bassina biotic pesticide to silkworm[J].Guangxi Agricultural Sciences,2010,10(1):1131-1133(in Chinese)
• [21]骆海玉,邓业成,廖永梅,等.不同球孢白僵菌菌株生物学特性与其对家蚕致病力的关系[J].安徽农业科学,2012,40(28):13799-13801,13803Luo H Y,Deng Y C,Liao Y M,et al.Relationship between biological characteristics of Beauveria bassiana Vuill and pathogenicity to Bombyx mori L.[J].Journal of Anhui Agricultural Sciences,2012,40(28):13799-13801,13803(in Chinese)