索丽娟;王宏元;罗磊;高学斌;赵洪峰;

• 1:陕西省动物研究所
• 2:陕西师范大学生命科学学院

摘要(Abstract)：

镉(cadmium,Cd)是一种典型的工业毒素和环境污染物,对两栖类生物具有强烈的毒性作用。为了探讨镉对两栖类生物的毒害作用,选择中华蟾蜍蝌蚪作为受试生物,研究了不同浓度Cd对中华蟾蜍蝌蚪生长、变态和Dios、TRs表达以及肝脏的的影响,结果表明,500μg·L~(~(-1))Cd可以极显著降低蝌蚪的变态率、全长、尾长和体重(P<0.01);Real-time PCR结果显示,在变态高峰期,500μg·L~(~(-1))Cd可以显著下调Dio2、TRα和TRβ mRNA的相对表达量(P<0.05),显著上调Dio3 mRNA的相对表达量(P<0.05);透射电镜试验结果显示Cd可以破坏中华蟾蜍蝌蚪肝脏细胞胞浆中的细胞器和胆管中的微绒毛。综合以上结果,可知,高浓度的Cd可以紊乱甲状腺的内分泌和导致肝脏的病变,进而影响中华蟾蜍蝌蚪的生长和变态。

关键词(KeyWords)： 镉;中华蟾蜍蝌蚪;变态;肝脏

Abstract:

Keywords:

基金项目(Foundation): 陕西省科学院重大项目(2013K-01)资助;; 国家自然科学基金(31201726)

作者(Author): 索丽娟;王宏元;罗磊;高学斌;赵洪峰;

Email:

DOI:

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