赵丽娟;张洪;解静芳;刘瑞卿;李萌;刘佩佳;王雅帅;

• 1:山西大学环境与资源学院
• 2:山西省分析科学研究院

摘要(Abstract)：

通过盆栽试验,评价土壤长残效期除草剂氯磺隆与重金属镉单一污染及复合污染对后茬作物菠菜生长、代谢产物的影响。基于GC-MS技术对3个不同处理组菠菜的代谢产物进行了辨识和分析。主成分分析结果表明,3个处理组的代谢物含量存在显著差异。具体表现为:1)与空白对照相比,氯磺隆诱导菠菜多种氨基酸、与能量代谢相关的3种糖类物质、苹果酸、γ-氨基丁酸、乳酸和腐胺的含量显著上升(*P<0.05);肌醇显著下降(*P<0.05);镉诱导菠菜多种氨基酸、与能量代谢相关的5种糖类物质、参与三羧酸循环的苹果酸和柠檬酸含量显著上升(*P<0.05);肌醇和腐胺含量显著下降(*P<0.05)。2)与单一污染相比较,复合污染诱导菠菜多种氨基酸,多种糖类物质和苹果酸等含量显著下降(*P<0.05)。由此可见,复合污染减弱了氯磺隆和镉单一污染时对氨基酸代谢和能量代谢的上调作用;氯磺隆和镉之间没有协同作用,相反对某些特定的代谢物存在拮抗作用。

关键词(KeyWords)： 氯磺隆;镉;菠菜;代谢物;GC-MS

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.30740037);; 国家公益性行业(农业)科研专项(No.201103024);; 山西省研究生优秀创新项目(011452901009);; 山西省自然科学基金(2014011013-2)

作者(Author): 赵丽娟;张洪;解静芳;刘瑞卿;李萌;刘佩佳;王雅帅;

Email:

参考文献(References)：

• [1]Khan S U.Bound residues in soil and plants[J].Residues Reviews,1982,84:1–25
• [2]IAEA.Quantification,nature and bioavailability of bound14C-pesticide residues in soil,plants and food[R].Vien-na,Austria:International Atomic Energy Agency,1986
• [3]Dec J,Bollag J M.Microbial release and degradation of cathecol and chlorophenols bound to synthetic humic acid[J].Soil Science Society of America Journal,1988,52:1366–1371
• [4]Ye Q F,Wu J M,Sun J H.Bioavailability of bound residue derived from 14C-labeled chlorsulfuron in soil and its mechanism of phytotoxicity[J].Journal of Environmental Sciences(China),2004,16:262–267
• [5]单正军.氯磺隆的环境行为及其对作物的影响[J].农药科学与管理,1998(3):12-14Shan Z J.Reviews on the environmental behavior of chlorsulfuron and its safety to plants[J].Pesticide Science and Adminstration,1998(3):12-14(in Chinese)
• [6]Hollaway K L,Kookana R S,Noy D M,et al.Persistence and leaching of sulfony-lurea herbicides over a 4-year period in the highly alkaline soils of south-eastern Australia[J].Australian Journal of Experimental Agriculture,2006,46:1069–1076
• [7]Giardina M C,Carosi S.Effects of chlorsulfuron on polyamine content in maize seedlings[J].Pesticide Biochemistry and Physiology,1990,36:229–236
• [8]栗献峰.太原市污灌区土壤重金属分布特征及风险评价[J].山西农业科学,2012,40(7):42-46,74Li X F.Distribution characteristics and risk evaluation of heavy metals in soil of sewage irrigation area of Taiyuan City[J].Journal of Shanxi Agricultural Sciences,2012,40(7):42-46,74(in Chinese)
• [9]Wagner G J.Accumulation of cadmium in crop plants and its consequences to human health[J].Advances in Agronomy,1993,1(51):173-212
• [10]Baszynski T,Krupa Z.Some aspects of heavy metals toxicity towards photosynthetic apparatus-direct and indirect effects on light and dark reactions[J].Acta Physiologiae Plantarum,1995,17:177–190
• [11]Das P,Samantaray S,Rout G.Studies on cadmium toxicity in plants:A review[J].Environmental Pollution,1997,98:29–36
• [12]Padmaja K,Prasad D,Prasad A.Inhibition of chlorophyll synthesis in Phaseolus vulgaris L.seedlings by cadmium acetate[J].Photosynthetica,1990,24:399–405
• [13]Pietrini F,Iannelli M A,Pasqualini S,et al.Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis(Cav.)Trin.ex Steudel[J].Plant Physiology,2003,133:829–883
• [14]Baryla A,Carrier P,Franck F,et al.Leaf chlorosis in oil seed rape plants(Brassica napus)grown on cadmium-polluted soil:Causes and consequences for photosynthesis and growth[J].Planta,2001,212:696–709
• [15]Larsson E H,Bornman J F,Asp H.Influence of UV-B radiation and Cd2+on chlorophyll fluorescence,growth and nutrient content in Brassica napus[J].Journal of Experimental Botany,1998,49:1031–1039
• [16]Bddi B,Oravecz A,Lehoczki E.Effect of cadmium on organization and photoreduction of protochlorphyllide in darkgrown leaves and etioplast inner membrane preparations of wheat[J].Photosynthetica(Czech Republic),1995,31:411–420
• [17]International Organization for Standardization.Soil quality–determination of the effects of pollutants on soil flora.Part 2:Effects of contaminated soil on the emergence and early growth of higher plants.BS EN ISO 11269-2[R].Geneva,Switzerland:ISO,2012
• [18]Xie Y,Hu L X,Du Z M,et al.Effects of cadmium exposure on growth and metabolic profile of bermudagrass[Cynodon dactylon(L.)Pers.][J].PLo S One,2014,9(12):e115279
• [19]范志金,钱传范,于维强,等.氯磺隆和苯磺隆对玉米乙酰乳酸合成酶抑制作用的研究[J].中国农业科学,2003,36(2):173-178Fan Z J,Qian C F,Yu W Q,et al.Study on enzymatic inhibition of acetolactate synthase from maize(Zea mays L.)by chlorsulfuron and tribenuron-methyl[J].Scientia Agricultura Snica,2003,36(2):173-178(in Chinese)
• [20]Zabalza A,Orcaray L,Gaston S,et al.Carbohydrate accumulation in leaves of plants treated with the herbicide chlorsulfuron or imazethapyr is due to a decrease in sink strength[J].Journal of Agricultural and Food Chemistry,2004,52:7601–7606
• [21]Zabalza A,Gonzalez E M,Arrese-Igor C,et al.Fermentative metabolism is induced by inhibiting different enzymes of the branched-chain amino acid biosynthesis pathway in pea plants[J].Journal of Agricultural and Food Chemistry,2005,53:7486–7493
• [22]Zhou Q,Liu W,Zhang Y,et al.Action mechanisms of acetolactate synthase-inhibiting herbicides[J].Pesticide Biochemistry and Physiology,2007,89:89–96
• [23]Orcaray L,Igal M,Marino D,et al.The possible role of quinate in the mode of action of glyphosate and acetolactate synthase inhibitors[J].Pest Management Science,2010,66:262–269
• [24]Orcaray L,Igal M,Zabalza A,et al.Role of exogenously supplied ferulic and p-coumaric acids in mimicking the mode of action of acetolactate synthase inhibiting herbicides[J].Journal of Agricultural and Food Chemistry,2011,59:10162–10168
• [25]Zabalza A,Amaia Z,Gil-Monreal M,et al.Branchedchain amino acid biosynthesis inhibitors:Herbicide efficacy is associated with an induced carbon-nitrogen imbalance[J].Journal of Plant Physiology,2013,170:814-821
• [26]Fayeaz K A,Kristen U.The influence of herbicides on the growths and proline content of primary roots and on the ultrastructure of root caps[J].Environmental and Experimental Botany,1996,36:71-81
• [27]Deng F.Effects of glyphosate,chlorsulfuron,and methyl jasmonate on growth and alkaloid biosynthesis of jimsonweed(Datura stramonium L.)[J].Pesticide Biochemistry and Physiology,2005,82:16-26
• [28]Xu J,Sun J,Du L,et al.Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum[J].New Phytologist,2012,196:110–124
• [29]Ho C L,Saito K.Molecular biology of the plastidic phosphorylated serine biosynthetic pathway in Arabidopsis thaliana[J].Amino Acids,2001,20:243–259
• [30]Waditee R,Bhuiyan N H,Hirata E,et al.Metabolic engineering for betaine accumulation in microbes and plants[J].Journal of Biological Chemistry,2007,282:34185–34193
• [31]Benstein R M,Ludewig K,Wulfert S,et al.Arabidopsis phosphoglycerate dehydrogenase1 of the phosphoserine pathway is essential for development and required for ammonium assimilation and tryptophan biosynthesis[J].Plant Cell,2013,25:5011–5029
• [32]刘清岱,朱晔荣,李洪涛,等.紫萍叶状体衰老过程中的内肽酶谱变化和外源L-丝氨酸对内肽酶谱的影响[J].植物生理学通讯,2010(3):241-244Liu Q D,Zhu Y R,Li H T,et al.Changes of endopeptidase isoenzymes in Spirodela polyrrhiza thallus during sene scence and effect of exogenous L-serine on endopeptidase isoenzymes[J].Plant Physiology Communications,2010(3):241-244(in Chinese)
• [33]Moya J,Ros R,Picazo I.Heavy metal-hormone interactions in rice plants:Effects on growth,net photosynthesis,and carbohydrate distribution[J].Journal of Plant Growth Regulation,1995,14:61–67
• [34]Kieffer P,Dommes J,Hoffmann L,et al.Quantitative changes in protein expression of cadmium-exposed poplar plants[J].Proteomics,2008,8:2514–2530
• [35]漆小泉,王玉兰,陈晓亚.主编.植物代谢组学-方法与应用[M].北京:化学工业出版社,2011Qi X Q,Wang Y L,Chen X Y.eds.Plant Metabolomics:Methods and Applications[M].Beijing:Chemical Industry Press,2011(in Chinese)
• [36]汪建飞,沈其荣.有机酸代谢在植物适应养分和铝毒胁迫中的作用[J].应用生态学报,2006,17(11):2210-2216Wang J F,Shen Q R.Roles of organic acid metabolism in plant adaptation to nutrient deficiency and a luminum toxicity stress[J].Chinese Journal of Applied Ecology,2006,17(11):2210-2216(in Chinese)
• [37]施征,史胜青,钟传飞,等.γ-氨基丁酸在植物抗逆生理及调控中的作用[J].生命科学研究,2007,11(4):57-61Shi Z,Shi S Q,Zhong C F,et al.The roles ofγ-aminobutyric acid on physiology and regulation under stress in plants[J].Life Science Research,2007,11(4):57-61(in Chinese)
• [38]王伟.植物激素及铝对小麦根尖有机酸代谢与分泌的影响[D].武汉:华中农业大学,2008Wang W.Effect of plant hormone and aluminum on organic acid metablism and secretion in wheat root tapcies[D].Wuhan:Huazhong Agricultural University,2008(in Chinese)
• [39]Song Y-J,Diao Q-N,Qi H-Y.Research progress on relationship between polyamine metabolism and plant stress resistance[J].China Vegetables,2012(1):36-42
• [40]Giardina M C,Carosi S.Effects of chlorsulfuron on polyamine content in maize seedlings[J].Pesticide Biochemistry and Physiology,1990,36:229-236
• [41]Yu L,Qin G,Zhang J,et al.D-myo-inositol-3-phosphate affects phosphatidylinositol-mediated endomembrane function in Arabidopsis and is essential for auxin-regulated embryogenesis[J].The Plant Cell,2011,23:1352-1372