四环素对微囊藻生长及光合作用的毒性效应机制Toxic Effects of Tetracycline on the Growth and Photosynthesis in Microcystis aeruginosa
董聪聪;张红波;杨燕君;付君珂;刘黎;贺新宇;施军琼;吴忠兴;
摘要(Abstract):
光合作用是蓝藻生长繁殖的生理基础,研究四环素胁迫下蓝藻的生长和光合作用的变化和响应,有助于揭示其作用机制。为探究四环素对微囊藻的毒性效应,对培养在0、0.1、0.2、0.5、1.0、2.0和5.0 mg·L~(-1)共7个浓度下的微囊藻的细胞数、叶绿素a含量、微囊藻光合系统Ⅱ(PSⅡ)快速光响应曲线(RLCs)及快速叶绿素荧光诱导动力学曲线(OJIP)进行了测定。结果表明,四环素处理7 d后,微囊藻的半致死浓度(LC_(50))为(0.571±0.036) mg·L~(-1)。随着四环素浓度的增加,微囊藻生长受到抑制,叶绿素含量减少,PSⅡ中单位反应中心失活。当四环素浓度高于0.5 mg·L~(-1)时,微囊藻Q_A~-到Q_B的电子传递被抑制,造成Q_A~-大量积累,影响PSⅡ的电子受体侧。然而,在低浓度四环素处理中,以吸收光能为基础的性能指数(PI_(ABS))、用于电子传递的量子产额(φ_(Eo))和最大光化学效率(φ_(Po))显著升高,用于热耗散的量子比率(φ_(Do))显著降低。这些结果表明,低浓度四环素处理时,微囊藻能通过自身调节改变PSⅡ中能量配置,改变电子传递速率,提高光合效率,从而应对低浓度四环素胁迫,而当四环素浓度较高时,微囊藻的光合作用显著降低,生长受到抑制。
关键词(KeyWords): 四环素;铜绿微囊藻;光合作用;毒理效应
基金项目(Foundation): 中央高校基本业务费专项资金项目(XDJK2019C044);; 西南大学博士基金项目(SWU110065)
作者(Author): 董聪聪;张红波;杨燕君;付君珂;刘黎;贺新宇;施军琼;吴忠兴;
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参考文献(References):
- [1]王敏,路福平,王建玲,等.四环素生产菌诱变育种与发酵性能的研究[J].中国抗生素杂志, 2000, 25(2):97-99Wang M, Lu F P, Wang J L, et al. Study on the breeding of high tetracycline producing strains and their fermentation characteristics[J]. Chinese Journal of Antibiotics,2000, 25(2):97-99(in Chinese)
- [2]李伟明,鲍艳宇,周启星.四环素类抗生素降解途径及其主要降解产物研究进展[J].应用生态学报, 2012, 23(8):2300-2308Li W M, Bao Y Y, Zhou Q X. Degradation pathways and main degradation products of tetracycline antibiotics:Research progress[J]. Chinese Journal of Applied Ecology,2012, 23(8):2300-2308(in Chinese)
- [3]Ye J, Du Y, Wang L, et al. Toxin release of cyanobacterium Microcystis aeruginosa after exposure to typical tetracycline antibiotic contaminants[J]. Toxins, 2017, 9(2):53-67
- [4]Schnappinger D, Hillen W. Tetracyclines:Antibiotic action, uptake, and resistance mechanisms[J]. Archives of Microbiology, 1996, 165(6):359-369
- [5]徐冬梅,王艳花,饶桂维.四环素类抗生素对淡水绿藻的毒性作用[J].环境科学, 2013, 34(9):3386-3390Xu D M, Wang Y H, Rao G W. Cellular response of freshwater green algae to the toxicity of tetracycline antibiotics[J]. Environmental Science, 2013, 34(9):3386-3390(in Chinese)
- [6]Xie X J, Zhou Q X, He Z C, et al. Physiological and potential genetic toxicity of chlortetracycline as an emerging pollutant in wheat(Triticum aestivum L.)[J]. Environmental Toxicology and Chemistry, 2010, 29(4):922-928
- [7]Gu C, Karthikeyan K G. Interaction of tetracycline with aluminum and iron hydrous oxides[J]. Environmental Science and Technology, 2005, 39(8):2660-2667
- [8]Sarmah A K, Meyer M T, Boxall A B. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics(VAs)in the environment[J]. Chemosphere, 2006, 65(5):725-759
- [9]Farkas M H, Berry J O, Aga D S. Chlortetracycline detoxification in maize via induction of glutathione S-transferases after antibiotic exposure[J]. Environmental Science and Technology, 2007, 41(4):1450-1456
- [10]Hamscher G, Sczesny S, H?per H, et al. Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry[J]. Analytical Chemistry, 2002, 74(7):1509-1518
- [11]Miao X S, Bishay F, Chen M, et al. Occurrence of antimicrobials in the final effluents of wastewater treatment plants in Canada[J]. Environmental Science and Technology, 2004, 38(13):3533-3541
- [12]那广水,陈彤,张月梅,等.中国北方地区水体中四环素族抗生素残留现状分析[J].中国环境监测, 2009, 25(6):78-80Na G S, Chen T, Zhang Y M, et al. Analysis terracyclines antibiotics residues status in water of north China[J]. Environmental Monitoring in China, 2009, 25(6):78-80(in Chinese)
- [13]国彬,姚丽贤,刘忠珍,等.广州市兽用抗生素的环境残留研究[J].农业环境科学学报, 2011, 30(5):938-945Guo B, Yao L X, Liu Z Z, et al. Environmental residues of veterinary antibiotics in Guangzhou City, China[J].Journal of Agro-Environment Science, 2011, 30(5):938-945(in Chinese)
- [14]姜蕾,陈书怡,杨蓉,等.长江三角洲地区典型废水中抗生素的初步分析[J].环境化学, 2008, 27(3):371-374Jang L, Chen S Y, Yang R, et al. Occurrence of antibiotics in the aquatic environment of the Changjiang Delta, China[J]. Environmental Chemistry, 2008, 27(3):371-374(in Chinese)
- [15]焦少俊,窦艳艳,陈良燕,等.四环素对苦草(Vallisneria natans)生长及细胞超微结构的影响[J].环境化学,2008, 27(3):335-338Jiao S J, Dou Y Y, Chen L Y, et al. Effect of aqueous tetracycline on Vallisneria natans in growth and ultrastructure[J]. Environmental Chemistry, 2008, 27(3):335-338(in Chinese)
- [16]张浩,罗义,周启星.四环素类抗生素生态毒性研究进展[J].农业环境科学学报, 2008, 27(2):407-413Zhang H, Luo Y, Zhou Q X. Research advancement of eco-toxicity of tetracycline antibiotics[J]. Journal of Agro-Environment Science, 2008, 27(2):407-413(in Chinese)
- [17]刘锋,陶然,应光国,等.抗生素的环境归宿与生态效应研究进展[J].生态学报, 2010, 30(16):4503-4511Liu F, Tao R, Ying G G, et al. Advance in environmental fate and ecological effects of antibiotics[J]. Acta Ecologica Sinica, 2010, 30(16):4503-4511(in Chinese)
- [18]Chalew T E, Halden R U. Environmental exposure of aquatic and terrestrial biota to triclosan and triclocarban[J].Journal of the American Water Resources Association,2009, 45(1):4-13
- [19]Yang W, Tang Z, Zhou F, et al. Toxicity studies of tetracycline on Microcystis aeruginosa, and Selenastrum capricornutum[J]. Environmental Toxicology and Pharmacology, 2013, 35(2):320-324
- [20]姜蕾,陈书怡,尹大强.四环素对铜绿微囊藻光合作用和抗氧化酶活性的影响[J].生态与农村环境学报,2010, 26(6):564-567Jiang L, Chen S Y, Yin D Q. Effects of tetracycline on photosynthesis and antioxidant enzymes of Microcystis aeruginosa[J]. Journal of Ecology and Rural Environment, 2010, 26(6):564-567(in Chinese)
- [21]王丽红,孙静雯,王雯,等.酸雨对植物光合作用影响的研究进展[J].安全与环境学报, 2017, 17(2):775-780Wang L H, Sun J W, Wang W, et al. Research advances in effects of acid rain on plant photosynthesis[J]. Journal of Safety and Environment, 2017, 17(2):775-780(in Chinese)
- [22]Ichimura T. Isolation and Culture Methods of Algae[M]//Nishizawa K, Chihara M. Eds. Methods in Phycological Studies. Tokyo:Kyoritsu Shuppan, 1979:294-305
- [23]Nush E. Comparison of different methods for chlorophyll and phaeopigment determination[J]. Archiv für Hydrobiologie-Beiheft Ergebniss der Limnologie, 1980, 14:14-36
- [24]雷玉新,刘耀兴,席银,等.水体中铁离子和氮磷比对藻类生长影响研究[J].生态科学, 2016, 35(1):75-78Lei Y X, Liu Y X, Xi Y, et al. Study on effects of iron ion and nitrogen-phosphorus ratio on the growth of algae[J].Ecological Science, 2016, 35(1):75-78(in Chinese)
- [25]周绪申,马帅领,尚志鸣,等.分光光度法测定铜绿微囊藻细胞密度研究[J].水利技术监督, 2016, 24(5):50-51Zhou X S, Ma S L, Shang Z M, et al. Determination of cell density of Microcystis aeruginosa by spectrophotometry[J]. Technical Supervision in Water Resources, 2016,24(5):50-51(in Chinese)
- [26]李翠萍,吴民耀,王宏元. 3种半数致死浓度计算方法之比较[J].动物医学进展, 2012, 33(9):89-92Li C P, Wu M Y, Wang H Y. LC50caculated by Kochi,probit analysis and linear regression methods[J]. Progress in Veterinary Medicine, 2012, 33(9):89-92(in Chinese)
- [27]Ralph P J, Gademann R. Rapid light curves:A powerful tool to assess photosynthetic activity[J]. Aquatic Botany,2005, 82(3):222-237
- [28]Platt T, Gallegos C L, Harrison W G. Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton[J]. Journal of Marine Research, 1980, 38(57):341-345
- [29]Liu R, Ran X F, Bai F, et al. Use of chlorophyll a fluorescence to elucidate the toxicity target of N-phenyl-2-naphthylamine on photosynthetic system of Cylindrospermopsis raciborskii(Cyanobacteria)[J]. Phycologia, 2015, 4(1):12-19
- [30]Strasser B J, Strasser R J. Measuring fast fluorescence transients to address environmental questions:The JIP-test[J]. Photochemistry Photobiology, 1995, 61:32-42
- [31]郭晓,李国良,刘孝利,等.梅江流域沉积物中四环素类抗生素的空间分布特征及其迁移转化规律[J].环境科学学报, 2015, 35(10):3202-3209Guo X, Li G L, Liu X L, et al. Spatial distribution, transportation and transformation of tetracyclines antibiotics in Meijiang River catchment[J]. Acta Scientiae Circumstantiae, 2015, 35(10):3202-3209(in Chinese)
- [32]赵素芬,曹日波.土霉素、氯霉素对湛江等鞭金藻细胞增殖和光合色素含量的影响[J].广东海洋大学学报,2004, 24(3):19-23Zhao S F, Cao R B. Effects of terramycin and ramphenicol on the growth of Isochrysis zhanjiangensis[J]. Journal of Zhanjiang Ocean University, 2004, 24(3):19-23(in Chinese)
- [33]周旭东.四环素类、磺胺类抗生素对铜绿微囊藻和小球衣藻的影响研究[D].杨凌:西北农林科技大学,2017:10-24Zhu X D. Effects of tetracyclines and sulfonamides on Microcystis aeruginosa and Chlamydomonas microsphaera[D]. Yangling:Northweat A&F University, 2017:10-24(in Chinese)
- [34]Kühne M, Ihnen D, M?ller G, et al. Stability of tetracycline in water and liquid manure[J]. Journal of Veterinary Medicine A, Physiology Pathology Clinical Medicine,2000, 47(6):379-384
- [35]姜思,刘莹莹,佟少明. 4种常用抗生素对莱茵衣藻生长及光化学活性的影响[J].生物加工过程, 2017, 15(2):13-20Jiang S, Liu Y Y, Tong S M. Effects of four antibiotics on growth and photochemical activities of Chlamydomonas reinhardtii[J]. Chinese Journal of Bioprocess Engineering,2017, 15(2):13-20(in Chinese)
- [36]Wei Z, Chen H, Guo L, et al. Effects of linear alkylbenzene sulfonate(LAS)on the interspecific competition between Microcystis, and Scenedesmus[J]. Environmental Science and Pollution Research International, 2016, 23(16):16194-16200
- [37]Qian H, Chen W, Li J, et al. The effect of exogenous nitric oxide on alleviating herbicide damage in Chlorella vulgaris[J]. Aquatic Toxicology, 2009, 92(4):250-257
- [38]Baumann H A, Morrison L, Stengel D B. Metal accumulation and toxicity measured by PAM—Chlorophyll fluorescence in seven species of marine macroalgae[J]. Ecotoxicology and Environmental Safety, 2009, 72(4):1063-1075
- [39]李华丽,唐永金,曾峰.铀和重金属复合污染对菊苣叶绿素荧光特性的影响[J].湖南师范大学自然科学学报,2015, 38(2):17-23Li H L, Tang Y J, Zeng F. Effects of multiple contamination of uranium and heavy metals on chlorophyll fluorescence characteristics of Cichorium intybus L.[J]. Journal of Natural Science of Hunan Normal University, 2015, 38(2):17-23(in Chinese)
- [40]邓培雁,刘威,韩博平.宝山堇菜(Viola baoshanensis)镉胁迫下的光合作用[J].生态学报, 2007, 27(5):1858-1862Deng P Y, Liu W, Han B P. Photosynthesis of Viola baoshanensis under Cd stress[J]. Acta Ecologica Sinica, 2007,27(5):1858-1862(in Chinese)
- [41]高培军.氮素施肥对毛竹光合能力与光谱特性的影响[D].北京:北京林业大学, 2013:56-67Gao P J. Effects nitrogen fertilization on photosynthetic capacity and spectral properties of Phyllostachys pubescens[D]. Beijing:Beijing Forestry University, 2013:56-67(in Chinese)
- [42]Maxwell K, Johnson G N. Chlorophyll fluorescence—A practical guide[J]. Journal of Experimental Botany, 2000,51(345):659-668
- [43]Krause G H, Weis E. Chlorophyll fluorescence and photosynthesis:The basics[J]. Annual Review of Plant Physiology, 1991, 42:313-349
- [44]张继旭,申国明,孔凡玉,等.四环素对烤烟生长发育及光合作用的影响研究[J].农业环境科学学报, 2017,36(1):48-56Zhang J X, Shen G M, Kong F Y, et al. Effect of tetracycline on the growth and photosynthesis of flue-cured tobacco[J]. Journal of Agro-Environment Science, 2017, 36(1):48-56(in Chinese)
- [45]Zhang D, Pan X, Mu G, et al. Toxic effects of antimony on photosystemⅡof Synechocystis sp. as probed by in vivo chlorophyll fluorescence[J]. Journal of Applied Phycology, 2010, 22(4):479-488
- [46]Müller P, Li X P, Niyogi K K. Non-photochemical quenching:A response to excess light energy[J]. Plant Physiology, 2001, 125(4):1558-1566
- [47]Force L, Critchley C, van Rensen J J S. New fluorescence parameters for monitoring photosynthesis in plants[J].Photosynthesis Research, 2003, 78(1):17-33
- [48]Krüger G H J, Tsimilli-Micheal M, Strasser R J. Light stress provokes plastic and elastic modifications in structure and function of photosystemⅡin camellia leaves[J]. Physiologia Plantarum, 1997, 101(2):265-277
- [49]王玉魁,郭慧媛,阎艳霞,等.酸雨胁迫对毛竹叶片光合速率和叶绿素荧光参数的影响[J].生态环境学报,2015, 24(9):1425-1433Wang Y K, Guo H Y, Yan Y X, et al. Effect of acid rain stress on photosynthetic rate and chlorophyll fluorescence parameters in leaves of Phyllostachys pubescens[J]. Ecology and Environmental Sciences, 2015, 24(9):1425-1433(in Chinese)
- [50]苏秀荣,王秀峰,杨凤娟,等.硝酸根胁迫对黄瓜幼苗叶片光合速率、PSⅡ光化学效率及光能分配的影响[J].应用生态学报, 2007, 18(7):1441-1446Su X R, Wang X F, Yang F J, et al. Effects of NO3-stress on photosynthetic rate, photochemical efficiency of PSⅡand light energy allocation in cucumber seedling leaves[J]. Chinese Journal of Applied Ecology, 2007, 18(7):1441-1446(in Chinese)
- [51]李鹏民,高辉远, Strasser R J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报, 2005, 31(6):559-566Li P M, Gao H Y, Strasser R J. Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study[J]. Journal of Plant Physiology and Molecular Biology, 2005, 31(6):559-566(in Chinese)
- [52]Xia J, Li Y, Zou D. Effects of salinity stress on PSⅡin Ulva lactuca, as probed by chlorophyll fluorescence measurements[J]. Aquatic Botany, 2004, 80(2):129-137
- [53]夏建荣,邹定辉.利用OJIP叶绿素a荧光评估干出对石莼(Ulva lactuca)光系统Ⅱ的影响[J].海洋通报, 2007,26(4):50-55Xia J R, Zou D H. Effects of desiccation on PSⅡperformance in Ulva lactuca assessed with OJIP chlorophyll a fluorescence transients[J]. Marine Science Bulletin,2007, 26(4):50-55(in Chinese)
- [54]Strasserf R J, Srivastava A. Polyphasic chlorophyll a, fluorescence transient in plants and cyanobacteria[J]. Photochemistry and Photobiology, 1995, 61(1):32-42
- [55]Dong H, Wei J M. Low concentrations of tetracycline enhance the photophosphorylation and P/O ratio of chloroplasts[J]. Photosynthesis Research, 2004, 79(2):201-208