樊磊;刘顺;包艳英;张英杰;刘颖;屈超;张治然;袭荣刚;王晓波;

• 1:中国人民解放军第210医院药学部
• 2:大连市环境监测中心

摘要(Abstract)：

采集大连城区大气中可吸入性细颗粒物(PM_(2.5)),研究其对肺癌A549细胞迁移、黏附和侵袭力的影响,利用明胶酶谱法检测细胞分泌的基质金属蛋白酶活性,利用Western blot法测定A549细胞中转移相关蛋白的表达。结果表明,在无明显细胞毒性浓度下,PM_(2.5)可增加A549细胞的迁移速率;细胞与细胞外基质的黏附率增加;细胞侵袭实验结果表明PM_(2.5)可增加A549细胞穿透基底膜的能力;用明胶酶谱法检测发现PM_(2.5)可使A549细胞分泌的基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)活性增强。转移相关蛋白——细胞钙粘蛋白-N(N-cadherin)的表达量升高,而钙粘蛋白-E(E-cadherin)的表达量下降,实验结果表明,PM_(2.5)可增强A549细胞的侵袭性,大气中的可吸入性颗粒可能导致肺癌转移发生率增加。

关键词(KeyWords)： PM_(2.5);肺癌细胞;细胞迁移;细胞侵袭;体外实验

Abstract:

Keywords:

基金项目(Foundation): 中国博士后科学基金面上项目(2015M572801)

作者(Author): 樊磊;刘顺;包艳英;张英杰;刘颖;屈超;张治然;袭荣刚;王晓波;

Email:

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