樊磊;刘顺;包艳英;张英杰;刘颖;屈超;张治然;袭荣刚;王晓波;

• 1:中国人民解放军第210医院药学部
• 2:大连市环境监测中心

摘要(Abstract)：

采集大连城区大气中可吸入性细颗粒物(PM_(2.5)),研究其对肺癌A549细胞迁移、黏附和侵袭力的影响,利用明胶酶谱法检测细胞分泌的基质金属蛋白酶活性,利用Western blot法测定A549细胞中转移相关蛋白的表达。结果表明,在无明显细胞毒性浓度下,PM_(2.5)可增加A549细胞的迁移速率;细胞与细胞外基质的黏附率增加;细胞侵袭实验结果表明PM_(2.5)可增加A549细胞穿透基底膜的能力;用明胶酶谱法检测发现PM_(2.5)可使A549细胞分泌的基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)活性增强。转移相关蛋白——细胞钙粘蛋白-N(N-cadherin)的表达量升高,而钙粘蛋白-E(E-cadherin)的表达量下降,实验结果表明,PM_(2.5)可增强A549细胞的侵袭性,大气中的可吸入性颗粒可能导致肺癌转移发生率增加。

关键词(KeyWords)： PM_(2.5);肺癌细胞;细胞迁移;细胞侵袭;体外实验

Abstract:

Keywords:

基金项目(Foundation): 中国博士后科学基金面上项目(2015M572801)

作者(Author): 樊磊;刘顺;包艳英;张英杰;刘颖;屈超;张治然;袭荣刚;王晓波;

Email:

DOI:

参考文献(References)：

• [1]Chen W,Zheng R,Baade P D,et al.Cancer statistics in China,2015[J].CA:A Cancer Journal for Clinicians,2016,66(2):115-132
• [2]张晓,杨琼英,林国桢,等.大气污染与居民肺癌发病及死亡灰色关联分析[J].中国公共卫生,2014,30(2):165-171Zhang X,Yang Q Y,Lin G Z,et al.Grey relational analysis on association between urban air pollution and lung cancer in China[J].Chinese Journal of Public Health,2014,30(2):165-171(in Chinese)
• [3]Anenberg S C,Horowitz L W,Tong D Q,et al.An estimate of the global burden of anthropogenic ozone and fine particulate matter on premature human mortality using atmospheric modeling[J].Environmental Health Perspectives,2010,118(9):1189-1195
• [4]Pope C A,Burnett R T,Thun M J,et al.Lung cancer,cardiopulmonary mortality,and long-term exposure to fine particulate air pollution[J].The Journal of the American Medical Association,2002,287(9):1132-1141
• [5]董雪玲.大气可吸入颗粒物对环境和人体健康的危害[J].资源产业,2004,6(5):50-53Dong X L.Impact of inhalable particles in atmosphere on environment and human health[J].Resources and Industries,2004,6(5):50-53(in Chinese)
• [6]Ying Z,Xie X,Bai Y,et al.Exposure to concentrated ambient particulate matter induces reversible increase of heart weight in spontaneously hypertensive rats[J].Particle and Fibre Toxicology,2015,12:15
• [7]Kim J Y,Lee E Y,Choi I,et al.Effects of the particulate matter 2.5(PM2.5)on lipoprotein metabolism,uptake and degradation,and embryo toxicity[J].Molecules and Cells,2015,38(12):1096-1104
• [8]楼煜清,韩宝惠.PM2.5对呼吸系统肿瘤发生发展的影响[J].临床内科杂志,2015,32(4):234-237Lou Y Q,Han B H.The effects of PM2.5on the occurrence of tumor development in respiratory system[J].Journal of Clinical Internal Medicine,2015,32(4):234-237(in Chinese)
• [9]Dai L,Mehta A,Mordukhovich I,et al.Differential DNA methylation and PM2.5species in a 450K epigenome-wide association study[J].Epigenetics,2017,12(2):139-148
• [10]Chu J H,Hart J E,Chhabra D,et al.Gene expression network analyses in response to air pollution exposures in the trucking industry[J].Environmental Health:A Global Access Science Source,2016,15(1):101-111
• [11]Frazier J L,Garonzik I M,Rhines L D.Metastatic Lung Cancer[M].Blackwell Publishing,Inc.,2008:199-220
• [12]吴鸿章,方来,麻庆乐,等.大气细颗粒物PM2.5对肺癌血管新生的影响[J].浙江中医药大学学报,2016,40(10):723-734Wu H Z,Fang L,Ma Q L,et al.Effect of PM2.5induced angiogenesis in lung cancer[J].Journal of Zhejiang Chinese Medical University,2016,40(10):723-734(in Chinese)
• [13]Fan L,Li Y,Sun Y,et al.Paris Saponin VII inhibits the migration and invasion in human A549 lung cancer cells[J].Phytotherapy Research,2015,29(9):1366-1372
• [14]Friedl P,Wolf K.Tumour-cell invasion and migration:Diversity and escape mechanisms[J].Nature Reviews Cancer,2003,3(5):362-374
• [15]Yang B,Chen D,Zhao H,et al.The effects for PM2.5exposure on non-small-cell lung cancer induced motility and proliferation[J].Springer Plus,2016,5(1):2059-2068
• [16]Chen L,Shi M,Gao S,et al.Assessment of population exposure to PM2.5for mortality in China and its public health benefit based on Ben MAP[J].Environmental Pollution,2017,221:311-317
• [17]Badyda A J,Grellier J,Dadrowiecki P.Ambient PM2.5exposure and mortality due to lung cancer and cardiopulmonary diseases in Polish cities[J].Advances in Experimental Medicine and Biology,2017,944:9-17
• [18]Wells A,Grahovac J,Wheeler S,et al.Targeting tumor cell motility as a strategy against invasion and metastasis[J].Trends in Pharmacological Sciences,2013,34(5):283-289
• [19]Seyfried T N,Huysentruyt L C.On the origin of cancer metastasis[J].Critical Reviews in Oncogenesis,2013,18(1-2):43-73
• [20]Shen H J,Sun Y H,Zhang S J,et al.Cigarette smokeinduced alveolar epithelial-mesenchymal transition is mediated by Rac1 activation[J].Biochimica et Biophysica Acta,2014,1840(6):1838-1849