SO_2衍生物对C57BL/6小鼠肝脏T细胞亚群CD4+/CD8+的影响Effect of SO_2 Derivatives on Liver T Lymphocytes Subsets CD4+/CD8+ of C57BL/6 Mice
武冬梅;李素清;狄晓艳;白剑英;
摘要(Abstract)：
为探讨二氧化硫(SO2)的肝脏免疫毒理效应,利用流式细胞仪(FACS)检测技术,分析了SO2体内代谢衍生物——亚硫酸钠(Na2SO3)与亚硫酸氢钠(NaHSO3)混合液(两者摩尔比为3:1)腹腔注射染毒对C57BL/6小鼠肝脏淋巴细胞T细胞亚群CD4+和CD8+的百分数以及CD4+/CD8+细胞比值的影响.实验组剂量分别为25、100、400mg·kg-1(body weight),染毒一周后,制备肝脏淋巴细胞悬液,经特异性荧光标记的CD4(FITC)、CD8(PE)单克隆抗体染色后,采用FACS流式细胞仪检测T淋巴细胞亚群百分数.研究发现:1)染毒后所有处理组肝脏CD4+T细胞所占的百分数显著升高(p<0.05);2)CD8+T细胞所占的百分数在100mg·kg-1、400mg·kg-1染毒组显著降低(p<0.05);3)CD4+/CD8+的比值在100mg·kg-1、400mg·kg-1染毒组显著升高(p<0.01).研究结果显示:SO2体内衍生物亚硫酸钠和亚硫酸氢钠可使肝脏CD4+/CD8+T细胞的比值显著升高,即SO2衍生物可使肝脏CD4和CD8淋巴细胞比例严重失调而使机体产生免疫紊乱.
关键词(KeyWords)： SO2衍生物;T淋巴细胞亚群;CD4+/CD8+;肝脏
基金项目(Foundation): 山西省自然科学基金项目(No.2009011039-2)
作者(Author): 武冬梅;李素清;狄晓艳;白剑英;
Email:
参考文献(References)：
- Andersson E,Nilsson T,Persson B,Wingren G,Torén K.1998.Mortality from asthma and cancer among sulfite mill workers[J].Scandinavian Journal of Work,Environment&Health,24(1):12-17
- Bai J Y,Meng Z Q.2002.Study of hepatic toxicity of short-term inhalation of sulfur dioxide[J].Chinese Hepatology,7(4):243-245(in Chinese)
- Bai J Y,Meng Z Q.2004.Sulfur dioxide-induced liver pathology[J].Chinese Journal of Pathology,33(2):74-76(in Chinese)
- Crapo J D.2003.Oxidative stress as an initiator of cytokinerelease and cell damage[J].European Respiratory Journal,22(Supplement44):4-6
- Dong Z J,Wei H M,Wang J,Luo D M,Zhang L,Chen Q F,Tian Z G.2002.Isolation and characteristics of murine hepaticinnate lymphocytes[J].Academic Journal of Second MilitaryMedical University,23(10):1095-1098(in Chinese)
- Haddad J.2002.Antioxidant and preoxidant mechanisms in theregulation of redox(Y)-sensitive transcription factors[J].CellularSignalling,14(11):879-897
- Hu R R.2001.Control and determination of antioxidant inamino acid injection[J].Academic Journal of Guangdong Collegeof pharmacy,17(4):283-284(in Chinese)
- Matanoski G,Kanchanaraksa S,Lees P,Tao X,Royall R,Francis M,Lantry D.1998.Industry-wide study of mortality ofpulp and paper mill workers[J].American Journal of IndustrialMedicine,33(4):354-365
- Meng Z Q.2003.Oxidative damage of sulfur dioxide on variousorgans of mice:sulfur dioxide is a systemic oxidative damageagent[J].Inhalation Toxicology,15(2):181-195
- Meng Z Q,Qin G H,Zhang B,Bai J L.2004.DNA damagingeffects of sulfur dioxide derivatives in cells from various organsof mice[J].Mutagenesis,19(6):465-468
- Meng Z Q,Li R J,Zhang X.2005a.Levels of sulfite in threeorgans from mice exposed to sulfur dioxide[J].InhalationToxicology,17(6):309-313
- Meng Z Q,Liu Y X,Wu D M.2005b.Effect of sulfur dioxideinhalation on cytokine levels in lungs and serum of mice[J].Inhalation Toxicology,17(6):303-307
- Meng Z Q,Qin G H,Zhang B.2005c.DNA damage in micetreated with sulfur dioxide by inhalation[J].Environmental andMolecular Mutagenesis,46(3):150-155
- Meng Z Q,Qin G H,Bai J L,Zhang J B,Yang Z H,Zhang X.2006.Alterations of genome expression profiles in rat lungexposed to sulfur dioxide:A evidence on the expression unsteadygenome to environmental pollutants[J].Journal of ShanxiUniversity(Natural Science Edition),29(3):225-236(in Chinese)
- Reus R,Houben G,Stam M,Debois A.2000.Food additives asa cause of medical symptoms:relationship shown between sulfitesand asthma and anaphylaxis;results of a literature review[J].Nederlands Tijdschrift Voor Geneeskunde,144(38):1836-1839
- Shapiro R.1977.Genetic effects of bisulfite(sulfur dioxide)[J].Mutatation Research Mutation Research/Reviews in GeneticToxicology,39(2):149-175
- Wu D M,Meng Z Q,Geng H,Bai W.2003.Effects ofsulphur dioxide inhalation on glutathione redox system in miceorgans[J].Chinese Journal of Applied&Environmental Biology,9(2):163-166(in Chinese)
- 白剑英,孟紫强.2002.短期二氧化硫吸入的肝脏毒性研究[J].肝脏,7(4):243-245
- 白剑英,孟紫强.2004.二氧化硫对肝脏组织学结构的影响[J].中华病理学杂志,33(2):74-76
- 董忠军,魏海明,王静,罗德明,张亮,陈清烽,田志刚.2002.小鼠肝脏天然免疫淋巴细胞分离及其特性研究[J].第二军医大学学报,23(10):1095-1098
- 胡容融.2001.氨基酸输液中抗氧化剂亚硫酸盐的控制与测定[J].广东药学院学报,17(4):283-284
- 孟紫强,秦国华,白居利,张建彪,杨振华,张欣.2006.二氧化硫对大鼠肺基因组表达谱的影响-环境表达不稳定基因组存在的证据[J].山西大学学报(自然科学版),29(3):225-236
- 武冬梅,孟紫强,耿红,白伟.2003.SO2吸入对小鼠谷胱甘肽氧化还原系统的影响[J].应用与环境生物学报,9(2):163-166