茚虫威对斑马鱼的急性毒性及遗传毒性Acute and Genetic Toxicity of Indoxacarb to Zebrafish(Brachydanio rerio)
冯青;赖柯华;黄伟康;刘禹杉;李江;章程辉;范咏梅;
摘要(Abstract):
茚虫威属噁二嗪类新型广谱高效杀虫剂,在农业生产中有着重要作用,近年来随着用量的增加,由此产生的对水生生物的影响越来越受到重视。使用斑马鱼进行茚虫威的急性毒性作用实验,并采用微核试验、彗星试验、Annexin V-FITC/PI双染色检测观察染毒后的斑马鱼是否被诱导产生遗传毒性。结果表明:在24 h、48 h、72 h、96 h,茚虫威LC50值分别为2.263mg·L-1、2.184 mg·L-1、2.184 mg·L-1、2.133 mg·L-1。茚虫威对斑马鱼急性毒性属中等毒性。96 h茚虫威浓度为1.98 mg·L-1和2.16 mg·L-1时,斑马鱼的微核率与对照组相比达差异极显著水平(P<0.01)。采用彗星试验检测斑马鱼肝脏DNA损伤,结果显示斑马鱼暴露于高浓度1.98 mg·L-1和2.16 mg·L-1茚虫威后,与对照组相比,斑马鱼的DNA损伤均表现为极显著差异(P<0.01)。采用Annexin V-FITC/PI双染色流式细胞仪法检测,结果表明染毒后24 h斑马鱼肝细胞出现细胞凋亡现象。实验结果进一步表明,茚虫威对斑马鱼短期染毒后诱导细胞凋亡,表现出遗传毒性。
关键词(KeyWords): 茚虫威;斑马鱼;急性毒性;遗传毒性;凋亡;微核试验;彗星试验;Annexin V-FITC/PI
基金项目(Foundation): 国家科技支撑计划项目(2012BAK01B05);; 海南省标准化项目(2012-004);; 海南省研究生创新科研课题(Hys2014-12)
作者(Author): 冯青;赖柯华;黄伟康;刘禹杉;李江;章程辉;范咏梅;
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