李晓东;袁思亮;李俊;刘春生;

• 1:华中农业大学水产学院

摘要(Abstract)：

17β-雌二醇(17β-estradiol,E2)是一种环境内分泌干扰物质,相关研究表明,E2对脊椎动物的生长和发育能够产生不良影响,但是E2对无脊椎动物,如大型溞(Daphnia magna)的毒性研究较少,其具体的毒性效应和致毒机制有待阐明。以E2为研究对象,以大型溞(<24 h)为实验动物,按照经济合作与发展组织(Economic Co-operation and Development,OECD)规定的标准试验程序开展为期21 d的标准暴露实验,评估不同浓度E2暴露对大型溞发育、生殖以及相关基因转录的影响。研究发现,1 360μg·L~(-1)E2暴露对大型溞产生了显著的致死效应,降低了大型溞蜕皮个数,增加了大型溞的产溞量,而其他低浓度的E2(1.36、13.6和136μg·L~(-1))暴露没有对大型溞产生显著的毒性效应。荧光定量PCR结果显示,1 360μg·L~(-1)E2暴露显著下调了大型溞蜕皮激素代谢相关基因细胞色素P450基因18a1(cytochrome P450 18a1,cyp18a1)和蜕皮激素受体基因(hormone receptor 3,hr3)的转录,说明E2可能是通过抑制大型溞的蜕皮进而引起了发育和生殖毒性。

关键词(KeyWords)： 雌二醇;大型溞;生殖毒性;发育毒性

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划资助项目(2017YFF0211203)

作者(Author): 李晓东;袁思亮;李俊;刘春生;

Email:

DOI:

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