莫正平;陈荣;黄火清;左正宏;

• 1:厦门大学环境与生态学院
• 2:近海海洋环境科学国家重点实验室
• 3:厦门大学生命科学学院

摘要(Abstract)：

从褐菖鲉肝脏中克隆了热休克蛋白HSC70基因,利用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)建立HSC70基因的定量检测方法。为检测该方法的可行性,将褐菖鲉分别暴露于石油水溶性成分(water-soluble fraction,WSF)20、60、180μg·L-11 d后,利用real-time PCR及LAMP技术同时测定褐菖鲉肝HSC70 mRNA表达量,两种方法测定结果基本一致,证实LAMP技术可用于褐菖鲉肝HSC70基因的定量。为更细致了解石油WSF影响褐菖鲉肝脏HSC70基因表达的剂量-效应关系,将褐菖鲉分别暴露于25、50、75、100、125、150、175μg·L-1WSF中,5 d后采样,用LAMP技术定量检测HSC70 mRNA。结果表明,HSC70 mRNA表达量在50μg·L-1浓度组即被显著诱导,在75μg·L-1浓度下达到最大值,这说明褐菖鲉肝HSC70基因对石油污染较敏感,有潜力作为海洋石油污染的生物标志物。

关键词(KeyWords)： 环介导等温扩增;褐菖鲉;HSC70;石油水溶性成分;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation): 海洋公益性项目"海洋污染生物效应快速监测与评价技术应用示范"子任务-海洋污染效应生物标志物技术集成与研发II(201005016)

作者(Author): 莫正平;陈荣;黄火清;左正宏;

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DOI:

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