氰戊菊酯对小白菜抗氧化酶活性及丙二醛(MDA)含量的影响Effects of Fenvalerate on the Activities of Antioxidant Enzymes,and Contents of Malondiadehyde (MDA) in Pakchoi
张清智;陈振德;刘红玉;
摘要(Abstract):
为探讨菊酯类农药胁迫对蔬菜生理生化过程的影响,以小白菜为研究对象,采用大田试验研究了不同浓度氰戊菊酯(20%乳油,推荐用量:稀释1500倍;加倍用量:稀释750倍)对小白菜抗氧化酶活性及丙二醛(MDA)含量的影响.结果表明:喷施推荐用量及喷施加倍用量氰戊菊酯对小白菜抗氧化酶活性均有不同程度的影响.超氧化物歧化酶(SOD)活性在喷施氰戊菊酯后3d内明显受到抑制,之后明显增加,第21d又受到抑制;过氧化氢酶(CAT)活性在喷药后小幅增加,第21d与对照基本一致;过氧化物酶(POD)活性在喷药后明显受到抑制.喷施推荐用量及喷施加倍用量氰戊菊酯均可引起小白菜MDA含量显著增加,且喷药浓度越高MDA含量越大,存在一定的剂量效应关系,表明氰戊菊酯胁迫促进了小白菜的膜脂过氧化作用.氰戊菊酯可对小白菜产生一定的氧化损伤,导致SOD、CAT、POD活性发生不同程度的变化.氰戊菊酯胁迫对SOD和POD活性以及MDA含量的影响相对较大,其中对MDA的影响最大,MDA含量可作为植物受到氰戊菊酯胁迫时的标记物.
关键词(KeyWords): 农药胁迫;小白菜;氰戊菊酯;抗氧化酶;丙二醛
基金项目(Foundation): 青岛市自然科学基金项目(No.06-2-2-16-jch)
作者(Author): 张清智;陈振德;刘红玉;
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DOI:
参考文献(References):
- Chen Z D,Chen X H,Feng M X,Yuan Y W.2005.Residuedynamics of chlorpyrifos in spinach[J].Journal of Agro-Environmental Science,24:728-731(in Chinese)
- Chen Z D,Yuan Y W,Chen X H,Feng M X,Lian Z X.2006.Residual dynamics of chlorpyrifos in Allium tuberosum[J].Journal of Safety and Environment,6:41-43(in Chinese)
- Chen Z D,Feng M X,Yuan Y W,Chen Y L,Lian Z X.2007.Research on residue dynamics laden in some commonlyused pesticides in spinach[J].Journal of Safety and Environment,7:1-4(in Chinese)
- Feng M X,Chen Z D,Yuan Y W,Chen X H,Lian Z X.2006.Residue degradation dynamics of several insecticides inonion[J].Agrochemicals,5:337-338,341(in Chinese)
- Feng M X,Chen Z D,Yuan Y W,Chen X H,Lian Z X.2007.Residue dynamics of pesticides in ridge cucumber[J].Pesticide Science and Administration,28:17-20(in Chinese)
- Feng X M,Lou S S,Hu J W,Wu J C,Wang Z G,Ma F.2003.Effect of pesticides on MDA and chlorophyll content ofrice leaves[J].Acta Agriculturae Nucleatae Sinica,16:481-484(in Chinese)
- Gao D X,Hao J C,Li Z F,Ma Y,Liu H F.2008.Effects ofHg stress on growth and physiological and biochemical characteristicsof rice seedlings[J].Journal of Agro-Environment Science,27:58-61(in Chinese)
- Gao Y W.2007.Analysis and countermeasure of pesticide residueand its environment pollution[J].Anhui Agricultural ScienceBulletin,13:130-131(in Chinese)
- Heng X M,Ma L,Heng H X,Zhao X L.2008.Evaluation ofthe efficacy and countermeasures about several acaricide totetranychus viennensis Zacher[J].Agrochemicals,47:68-69(inChinese)
- Huckaba R M,Cable H D,Van Duyn J W.1988.Joint effectsof acifluorfen applications and soybean thrips(Sericothripsvariabilis)feeding on soybean(Glycine max)[J].Weed Science,36:667-670
- Ji H F,Song R Q,Yang Q.2007.Effects of extraction fromLactarius vellereus fermenting liquor on the activity of protectiveenzymes,content of MDA and conductivity ratio in Alternariaalternata(Fr.)Keissler[J].Journal of Beijing ForestryUniversity,29:156-160(in Chinese)
- Jiang Y,Meng L,Zhao H J.2007.Influences of high temperaturewith soil drought on physiological character of tomato seedlings[J].Northern Horiticulture,(1):1-5(in Chinese)
- Li H S.2004.Principles and Techniques of Plant PhysiologicalBiochemical Experiment[M].Beijing:Higher Education Press(in Chinese)
- Li J W,Wang D.2008.The effect of water stress on theactivity of antioxidant enzymes of Solanum tuberosum L.[J].Northern Horticulture,(1):7-9(in Chinese)
- Liu H X,Zeng S G,Wang Y R,Li P,Chen D F.Guo J Y.1988.The effect of low temperature on superoxide dismutase invarious organelles of cucumber seedling cotyledon with differentcold tolerance[J].Acta Photophysiologica Sinica,11:48-57(inChinese)
- Liu J L,Yu J F,Yin J L,Wu J C.2006.Research progresson the effect of chemical pesticides on plant physiology andbiochemistry[J].Agrochemicals,45:511-514(in Chinese)
- Liu Y,Wang L H,Wang Y S.2007.Removal the impact ofpesticides on soil environment[J].Energy and Environment,6:67-70(in Chinese)
- Liu Z Q,Yang Y H,Shen J Z.1994.The effect of photo-dependent herbicides on plant SOD activity[J].Acta AgriculturaeUniversitatis Pekinensis,20:157-159(in Chinese)
- Shen Y,Feng C C,Li S,Guo W S,Zhu X K,Peng Y X.2007.The effect of pesticides under the stress of drought onwheat physiological and biochemical characteristics[J].JiangsuAgricultural Sciences,3:16-19(in Chinese)
- Sun W J,Gao F,Wei S M,Guan J S,Yu W Q.2004.Effectof jasmine pollution on the amount of pesticide residue injasmine tea[J].Journal of Fujian Agriculture and ForestryUniversity(Natural Science Edition),33:343-347(in Chinese)
- Tang H F,Sheng X M,Xiong L,Luan C Y,Wang Y,Liu XP.2006.The impact of organophosphates pesticide to solubleproteins and several oxidant enzymes in vegetable[J].Journal ofCentral China Normal University(Natural Sciences Edition),40:82-85(in Chinese)
- Tang X M,Wang W Q,Zhang H Y,Li W D.2008.Effectson the leaf physiology traits of Glycyrrhiza uralensis underdifferent concentration NaCl processing[J].Chinese AgriculturalScience Bulletin,24:229-233(in Chinese)
- Wu J C,Dong B,Li D H,Qiu H M,Yang G Q.2004.Effects of four pesticides on grain growth parameters of rice[J].Scientia Agricultura Sinica,37:376-381(in Chinese)
- Wu J C,Xu J X,Yuan S Z,Liu J L,Jiang Y H,Xu J F.2001.Pesticide-induced susceptibility of rice to brown planthopperNilaparvata lugens[J].Entomologia Experimentalis et Applicata,100:119-126
- Xu D M,Ma Z Q,Feng J T,Fu J H,Wei H,Zhang X.2004.Effects of chlorpyrifos and podophyllotoxin on anti-oxidative enzymes and the MDA of vegetable(Brassica rapa L.)[J].Journal of Agro-Environmental Science,23:1089-1092(inChinese)
- Xue G X,Gao H Y,Li P M,Zou Q.2004.Effects of chitosantreatment on physiological and biochemical characteristics incucumber seedlings under low temperature[J].Acta Photophysi-ologica Sinica,30:441-448(in Chinese)
- Zhang Y L,Han D,Li X,Pang H Y.2008.Four kinds ofpesticides on the pine shoot borer control spot[J].Journal ofMudanjiang Teachers’College(Natural Sciences Edition),62:44-45(in Chinese)
- Zhang Y P,Lin X Y,Zhang Y S,Li G,Yang Y J.2006.Effect of nitrogen forms on the growth and antioxidative enzymeactivities of spinach[J].Journal of Zhejiang University(Agricultureand Life Sciences),32:139-144(in Chinese)
- Zhang Y X,Li X L.2003.Toxicity of three organophosphoruspesticides on hordeum vulgare seedling[J].Journal of Agro-Environmental Science,22:754-757(in Chinese)
- Zhao S J,Xu C C,Zou Q,Meng Q W.1994.Improvementsof method for measurement of malondialdehyde in plant tissues[J].Plant Physiology Communications,30:207-210(in Chinese)
- Zhao W X,Sun Z Q,Zhang N,Gao H Y.2007.Effect ofselected organophosphorus pesticides on allicin content in garlicand its reasons[J].Journal of Safety and Environment,7:13-15(in Chinese)
- 陈振德,陈雪辉,冯明祥,袁玉伟.2005.毒死蜱在菠菜中的残留动态研究[J].农业环境科学学报,24:728-731
- 陈振德,冯明祥,袁玉伟,陈元林,连之新.2007.几种常用农药在菠菜中的残留动态研究[J].安全与环境学报,7:1-4
- 陈振德,袁玉伟,陈雪辉,冯明祥,连之新.2006.毒死蜱在韭菜中的残留动态研究[J].安全与环境学报,6:41-43
- 冯明祥,陈振德,袁玉伟,陈雪辉,连之新.2006.几种常用杀虫剂在洋葱上的残留降解动态研究[J].农药,5:337-338,341
- 冯明祥,陈振德,袁玉伟,陈雪辉,连之新.2007.几种农药在露地黄瓜上的残留降解动态检测研究[J].农药科学与管理,28(5):17-20
- 冯绪猛,罗时石,胡建伟,吴进才,王泽港,马飞.2003.农药对水稻叶片丙二醛及叶绿素含量的影响[J].核农学报,16:481-484
- 高大翔,郝建朝,李子芳,马勇,刘惠芬.2008.汞胁迫对水稻生长及幼苗生理生化的影响[J].农业环境科学学报,27:58-61
- 高艳卫.2007.农药残留及其环境污染分析研究与对策[J].安徽农学通报,13:130-131
- 衡雪梅,马丽,衡红霞,赵学礼.2008.几种杀螨剂对山楂叶螨的防效评价及防治[J].农药,47:68-69
- 计红芳,宋瑞清,杨谦.2007.绒白乳菇发酵液提取物对杨树叶枯病菌保护酶活性、丙二醛含量及电导率的影响[J].北京林业大学学报,29:156-160
- 蒋燕,孟玲,赵会杰.2007.高温干旱处理对番茄不同品种幼苗生长的影响[J].北方园艺,(1):1-5
- 李合生.2004.植物生理生化实验原理和技术[M].北京:高等教育出版社
- 李建武,王蒂.2008.水分胁迫对马铃薯试管苗抗氧化酶活性的影响[J].北方园艺,(1):7-9
- 刘鸿先,曾韶国,王以柔,李平,陈德峰,郭俊彦.1985.低温对不同耐寒力的黄瓜(Cucumis sativus)幼苗子叶各细胞器中超氧化物歧化酶(SOD)的影响[J].植物生理学报,11:48-57
- 刘井兰,于建飞,印建莉,吴进才.2006.化学农药对植物生理生化影响的研究进展[J].农药,45:511-514
- 刘艳,王丽华,王玉灿.2007.农药对土壤环境的影响及其去除[J].能源与环境,6:67-70
- 刘支前,杨艳华,申继忠.1994.需光型除草剂对植物SOD活力的影响[J].北京农业大学学报,20:157-159
- 沈燕,封超年,李邵,郭文善,朱新开,彭永欣.2007.农药对干旱胁迫下小麦幼苗生理生化特性的影响[J].江苏农业科学,3:16-19
- 孙威江,高峰,危赛明,管爵杉,余文权.2004.茉莉花污染对花茶农药残留的影响[J].福建农林大学学报(自然科学版),33:343-347
- 唐红枫,生秀梅,熊丽,栾勇成,王媛,刘喜平.2006.有机磷农药对小白菜中可溶性蛋白质及SOD、Mg2+-ATPase、Ca2+-ATPase和CAT的影响[J].华中师范大学学报(自然科学版),40:82-85
- 唐晓敏,王文全,张红瑞,李卫东.2008.不同浓度NaCl处理对甘草叶片生理特性的影响[J].中国农学通报,24:229-233
- 吴进才,董波,李冬虎,邱慧敏,杨国庆.2004.4种农药对水稻籽粒生长模型参数的影响[J].中国农业科学,37:376-381
- 徐敦明,马志卿,冯俊涛,傅建炜,魏辉,张兴.2004.毒死蜱和鬼臼毒素胁迫对蔬菜上海青抗氧化酶系及丙二醛的影响[J].农业环境科学学报,23:1089-1092
- 薛国希,高辉远,李鹏民,邹琦.2004.低温下壳聚糖处理对黄瓜幼苗生理生化特性的影响[J].植物生理与分子生物学学报,30:441-448
- 张义贤,李晓玲.2003.三种有机磷农药对大麦毒性效应的研究[J].农业环境科学学报,22:754-757
- 张兰英,韩丹,李雪,逄宏扬.2008.4种农药对松梢斑螟的防治[J].牡丹江师范学院学报(自然科学版),62:44-45
- 张英鹏,林咸永,章永松,李刚,杨运娟.2006.不同氮素形态对菠菜生长及体内抗氧化酶活性的影响[J].浙江大学学报(农业与生命科学版),32:139-144
- 赵世杰,许长城,邹琦,孟庆伟.1994.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,30:207-210
- 赵卫星,孙治强,张楠,高玉红.2007.有机磷农药对大蒜素含量的影响及其原因[J].安全与环境学报,7:13-15