早期胚胎发育期暴露1-辛基-3-甲基咪唑离子液体后对金鱼仔鱼的氧化损伤Oxidative Stress of the Ionic Liquid 1-Octyl-3-Methylimidazolium Bromide on the Goldfish Larva Carassius auratus Hatching from the IL-treated Embryos
李效宇;皇培培;王春雨;
摘要(Abstract):
离子液体的生物毒性受到越来越多关注,但目前该方面的研究报道很少.采用溴化1-辛基-3-甲基咪唑离子液体([C8mim]Br)处理金鱼胚胎,研究了金鱼早期胚胎发育期暴露离子液体后对金鱼仔鱼的氧化损伤.通过预实验获得金鱼胚胎(卵裂期)72小时半致死浓度(LC50)为209mg·L-1,根据该结果设计胚胎发育期离子液体的亚慢性暴露浓度为10.45mg·L-1、20.9mg·L-1、41.5mg·L-1、104.5mg·L-1.实验结果表明,与对照组相比,低浓度离子液体处理组(10.45mg·L-1和20.9mg·L-1)超氧化物歧化酶活性显著上升,高浓度处理组(41.55mg·L-1和104.55mg·L-1)极显著下降.谷胱苷肽过氧化物酶活性除10.455mg·L-1处理组为不显著上升外,其余3组均为显著下降.4个处理组丙二醛含量均比对照组显著增高,且呈现明显的剂量-效应关系.该实验结果表明,经过胚胎发育期暴露离子液体后,[C8mim]Br对金鱼仔鱼组织抗氧化系统仍产生较强的氧化损伤并引起脂质过氧化作用.因此,有必要研究离子液体对水生态系统中水生动物种群的影响,并对离子液体的生态安全做出科学评价.
关键词(KeyWords): 离子液体;金鱼;氧化损伤
基金项目(Foundation): 国家自然科学基金(No.20573019;No.20573034);; 教育部留学回国人员科研启动基金(No.[2006]331)
作者(Author): 李效宇;皇培培;王春雨;
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