邻苯二甲酸丁基苄酯致小鼠肥大细胞DNA损伤的初步研究A Preliminary Study on DNA Damages of Mouse Mast Cells Induced by Butyl Benzyl Phthalate
唐蓓;吴凯;吴丹;常青;刘丹丹;袁均林;
摘要(Abstract)：
为探讨邻苯二甲酸丁基苄酯(BBP)对小鼠肥大细胞DNA的损伤作用,采用不同浓度BBP(0、4、20、100μmol·L-1)对体外培养的小鼠肥大细胞染毒60min,应用单细胞凝胶电泳法和KCl-SDS沉淀法检测细胞DNA断裂和DNA-蛋白质交联.结果表明,低、中、高浓度(4、20、100μmol·L-1)的BBP均可引起小鼠肥大细胞DNA损伤,低浓度BBP引起的DNA损伤主要以DNA断裂为主,而中、高浓度BBP引起的DNA损伤主要以DNA-蛋白质交联为主。
关键词(KeyWords)： 邻苯二甲酸丁基苄酯;DNA断裂;DNA-蛋白质交联;小鼠;肥大细胞
基金项目(Foundation): 国家科技部建设部科技支撑计划项目(No.2006BAJ02A10);; 国家科技部卫生部科技支撑计划项目(No.2006BAI19B05)
作者(Author): 唐蓓;吴凯;吴丹;常青;刘丹丹;袁均林;
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