黑泽文;向慧敏;章家恩;梁开明;郅帅杰;赵敏;

• 1:华南农业大学资源环境学院
• 2:农业部华南热带农业环境重点实验室
• 3:广东省现代生态农业与循环农业工程技术研究中心
• 4:广东省生态循环农业重点实验室

摘要(Abstract)：

土壤重金属污染一直以来备受关注,作物能够吸收重金属从而对人体健康产生威胁,利用植物修复土壤重金属污染是当前环境科学和生态学领域的研究热点。本研究以一种水生豆科作物水合欢(Neptunia olerace)为研究对象,采用盆栽实验,研究水合欢对不同浓度Cd (0、50、100、180 mg·kg~(-1))、Pb (0、500、1 000、1 800 mg·kg~(-1))的耐受能力及吸收与富集情况。结果表明,(1)在Cd胁迫下,水合欢表现出一定的耐受性,生物量和植株长度与对照相比均显著降低(P<0.05),可溶性蛋白、叶绿素以及根和茎中的MDA含量与对照均无显著差异,叶片中CAT活性显著提高(P<0.05),但根中CAT、茎中SOD和叶片中POD活性及叶中MDA含量均先降低后升高;(2)在Pb胁迫下,水合欢表现出很强的耐受性,水合欢生物量、植株长度、可溶性蛋白、叶绿素和MDA含量与对照均无显著差异,但叶片中CAT活性显著提高(P<0.05),根和茎中SOD、茎中CAT和叶片中POD活性均先降低后升高(P<0.05);(3) Cd浓度为50、100、180 mg·kg~(-1)时,水合欢的富集系数分别为0.28、0.32和0.29,转运系数分别为0.05、0.06和0.08; Pb浓度为500、1 000、1 800 mg·kg~(-1)时,水合欢的富集系数分别为0.02、0.04和0.02,转运系数分别为0.04、0.08和0.05。水合欢对土壤中Cd、Pb的吸收均未达到超富集植物的标准,但本研究发现水合欢地下部重金属含量远高于地上部重金属含量,且水合欢为直根系植物,根系较浅,容易回收,基于此,可考虑将其与水稻等水生作物进行间套作,这种生产方式既可固氮,又可利用其根系来原位缓解土壤重金属污染对粮食等农产品生产所造成的安全风险。

关键词(KeyWords)： Cd;Pb;水合欢;重金属;酶活性

Abstract:

Keywords:

基金项目(Foundation): 广东省现代农业产业技术体系创新团队项目(2018LM1100,2019KJ105);; 广东省科技计划项目(2015B090903077,2016A020210094,2017A090905030,2019B030301007);; 广州市科技计划项目(201604020062)

作者(Author): 黑泽文;向慧敏;章家恩;梁开明;郅帅杰;赵敏;

Email:

DOI:

参考文献(References)：

• [1] Lin Y F,Aarts M G. The molecular mechanism of zinc and cadmium stress response in plants[J]. Cellular and Molecular Life Sciences,2012,69(16):3187-3206
• [2] Dalcorso G,Farinati S,Furini A. Regulatory networks of cadmium stress in plants[J]. Plant Signaling and Behavior,2010,5(6):663-667
• [3] Fontes M P F,Alleoni L R F. Electrochemical attributes and availability of nutrients,toxic elements,and heavy metals in tropical soils[J]. Scientia Agricola,2006,63(6):589-608
• [4] Przedpeska E,Wierzbicka M. Arabidopsis arenosa(Brassicaceae)from a lead-zinc waste heap in southern Poland—A plant with high tolerance to heavy metals[J]. Plant and Soil,2007,299(1-2):43-53
• [5]尚二萍，张红旗，杨小唤，等．我国南方四省集中连片水稻田土壤重金属污染评估研究[J]．环境科学学报，2017,37(4):1469-1478Shang E P,Zhang H Q,Yang X H,et al. Assessment of soil heavy metal of paddy field in four provinces in southern China[J]. Acta Scientiae Circumstantiae,2017,37(4):1469-1478(in Chinese)
• [6]何兰兰，角媛梅，王李鸿，等．Pb、Zn、Cu、Cd的超富集植物研究进展[J]．环境科学与技术，2009,32(11):120-123He L L,Jiao Y M,Wang L H,et al. Advance in study of Pb,Zn,Cu and Cd hyperaccumulators[J]. Environmental Science and Technology,2009,32(11):120-123(in Chinese)
• [7] Wu M,Luo Q,Zhao Y. Physiological and biochemical mechanisms preventing Cd toxicity in the new hyperaccumulator Abelmoschus manihot[J]. Journal of Plant Growth Regulation,2018,37(3):709-719
• [8] Wu L,Zhou J,Zhou T. Estimating cadmium availability to the hyperaccumulator Sedum plumbizincicola in a wide range of soil types using a piecewise function[J]. Science of the Total Environment,2018,637-638:1342-1350
• [9] Lin L,Chen F,Wang J,et al. Effects of living hyperaccumulator plants and their straws on the growth and cadmium accumulation of Cyphomandra betacea seedlings[J]. Ecotoxicology and Environmental Safety,2018,155:109-116
• [10] Leal-Alvarado D A,Martínez-Hernández A,CalderónVázquez C L,et al. Identification of up-regulated genes from the metal-hyperaccumulator aquatic fern Salvinia minima Baker,in response to lead exposure[J]. Aquatic Toxicology,2017,193:86-96
• [11] Dinh N,van der Ent A,Mulligan D R,et al. Zinc and lead accumulation characteristics and in vivo distribution of Zn2+in the hyperaccumulator Noccaea caerulescens elucidated with fluorescent probes and laser confocal microscopy[J]. Environmental and Experimental Botany,2018,147:1-12
• [12] Yuan H Y,Huang S Z,Han Y L. The growth lead accumulation and oxidative stress response of Iris lactea var.chinensis under lead stress[J]. Fresenius Environmental Bulletin,2013,22(7):1887-1894
• [13] Lan X,Yang B,Yan Y,et al. Resistance mechanisms and their difference between the root and leaf of Microsorum pteropus—A novel potential aquatic cadmium hyperaccumulator[J]. Science of The Total Environment,2018,616-617:480-490
• [14]邢艳帅，乔冬梅，朱桂芬，等．土壤重金属污染及植物修复技术研究进展[J]．中国农学通报，2014,17(30):208-214Xing Y S,Qiao D M,Zhu G F,et al. Research progress of heavy pollution in soil and phytoremediation technology[J]. Chinese Agricultural Science Bulletin,2014,17(30):208-214(in Chinese)
• [15]韦革宏，马占强．根瘤菌-豆科植物共生体系在重金属污染环境修复中的地位、应用及潜力[J]．微生物学报，2010,50(11):1421-1430Wei G H,Ma Z Q. Application of rhizobia-legume symbiosis for remediation of heavy-metal contaminated soils[J]. Acta Microbiologica Sinica,2010,50(11):1421-1430(in Chinese)
• [16]樊妙春．刺槐-根瘤菌共生体系在重金属污染土壤修复过程中对土壤微生物的影响[D]．杨凌:西北农林科技大学，2017:6-9Fan M C. Effect of Robinia pseudoacacia-rhizobia sumbiosis on microbiome in the phytoremediation of heavy metal contaminated soil in mine area[D]. Yangling:Northwest Agriculture and Forestry University,2017:6-9(in Chinese)
• [17]张志权．土壤种子库与矿业废弃地植被恢复研究:定居植物对重金属的吸收和再分配[J]．植物生态学报，2001,25(3):306-311Zhang Z Q. Uptake and translocation of heavy metals in dominant plants of soil seed banks introduced to a lead/zinc mine tailngs pond[J]. Acta Phytoecolo Rica Sinica,2001,25(3):306-311(in Chinese)
• [18]孙伟，王琼，张望，等．湖南省铅锌尾矿污染区植被修复适生植物选择研究[J]．价值工程，2016,35(17):208-211Sun W,Wang Q,Zhang W,et al. Screening of vegetation for ecological restoration around lead-zinc tailings site in Hunan Province[J]. Value Engineering,2016,35(17):208-211(in Chinese)
• [19]张少斌，梁开明，张殷，等．水稻与水合欢间作对作物群体产量、氮素吸收及土壤氮素的影响[J]．生态环境学报，2016,25(11):1856-1864Zhang S S,Liang K M,Zhang Y,et al. Effects of rice and water mimosa intercropping on crop yield,nitrogen uptake and soil nitrogen content[J]. Ecology and Environmental Sciences,2016,25(11):1856-1864(in Chinese)
• [20] Wahab A,Ismail S S,Abidin E Z,et al. Neptunia oleracea(water mimosa)as phytoremediation plant and the risk to human health:A review[J]. Advances in Environmental Biology,2014,8(15):187-194
• [21]章家恩．生态学常用实验研究方法与技术[M]．北京:化学工业出版社，2007:75-78
• [22]中华人民共和国国家质量监督检验检疫总局．GB/T17138—1997土壤质量铜、锌的测定火焰原子吸收分光光度法[S]．北京:中国国家标准化管理委员会，1997
• [23]鲁如坤．土壤农业化学分析方法[M]．北京:中国农业科技出版社，2000:146-195
• [24] Adler A,Karacic A,Weih M. Biomass allocation and nutrient use in fast-growing woody and herbaceous perennials used for phytoremediation[J]. Plant and Soil,2008,305(1-2):189-206
• [25]吕金印，张微，柳玲．Cd2+处理对几种叶菜可食部分Cd含量及品质的影响[J]．核农学报，2010,24(4):856-862Lv J Y,Zhang W,Liu L. Effect of cadmium treatment in Cd absorption and nutrition quality of edible part in different leaf vegetables[J]. Journal of Nuclear Agricultural Sciences,2010,24(4):856-862(in Chinese)
• [26]湛灵芝，铁柏清，曾桂华．Cd2+、乙草胺单一及其复合污染对少根紫萍生理生化特性的影响[J]．安全与环境学报，2014,13(3):9-13Zhan L Z,Tie B Q,Zeng G H. Single and the synthetic effects of Cd2+and the acetochlor on the physiological behaviors of Spirodela oligorrhiza[J]. Journal of Safety and Environment,2014,13(3):9-13(in Chinese)
• [27]龚小敏，刘云国，黄丹莲，等．外源钙对镉胁迫下苎麻生长及生理代谢的影响[J]．环境工程学报，2016,10(7):3866-3870Gong X M,Liu Y G,Huang D L,et al. Effects of exogenous calcium on plant growth and physiological metabolism of Boehmeria nivea(L.)Gaudich under cadmium stress[J]. Chinese Journal of Environmental Engineering,2016,10(7):3866-3870(in Chinese)
• [28] Syuhaida A W A,Norkhadijah S I S,Praveena S M,et al. The comparison of phytoremediation abilities of water mimosa and water hyacinth[J]. ARPN Journal of Science and Technology,2014,4(12):722-731
• [29]徐佳佳，刘宁宁，秦丽，等．镉胁迫对续断菊与玉米间作体系中植物叶片抗氧化酶活性的影响[J]．云南农业大学学报:自然科学，2016,30(2):348-355Xu J J,Liu N N,Qin L,et al. Effects of Cd stress on antioxidant enzymes activity of Souchus asper L. Hill. and Zea mays L. in intercropping system[J]. Journal of Yunnan Agricultural University:Natural Science,2016,30(2):348-355(in Chinese)
• [30] Schutzendubel A,Polle A. Plant responses to abiotic stresses:Heavy metal-induced oxidative stress and protection by mycorrhization[J]. Journal of Experimental Botany,2002,53(372):1351-1365
• [31] Nagaraj M,Sunitha S,Varalakshmi P. Effect of lupeol,a pentacyclic triterpene,on the lipid peroxidation and antioxidant status in rat kidney after chronic cadmium exposure[J]. Journal of Applied Toxicology,2000,20(5):413-417
• [32]王晓维，黄国勤，徐健程，等．铜胁迫和间作对玉米抗氧化酶活性及丙二醛含量的影响[J]．农业环境科学学报，2014,33(10):1890-1896Wang X W,Huang G Q,Xu J C,et al. Effects of copper stresses and intercropping on antioxidant enzyme activities and malondialdehyde contents in maize[J]. Journal of Agro-Environment Science,2014,33(10):1890-1896(in Chinese)
• [33] Georgiadou E C,Kowalska E,Patla K,et al. Influence of heavy metals(Ni,Cu,and Zn)on nitro-oxidative stress responses,proteome regulation and allergen production in basil(Ocimum basilicum L.)plants[J].Frontiers in Plant Science,2018,9. DOI:10. 3389/fpls.2018.00862
• [34] Chowdhary P,Yadav A,Singh R,et al. Stress response of Triticum aestivum L. and Brassica juncea L. against heavy metals growing at distillery and tannery wastewater contaminated site[J]. Chemosphere,2018,206:122-131
• [35]陈海林．水葫芦的抗氧化研究[D]．广州:广东工业大学，2012:17-24Chen H L. Studies on antioxidation of water hyacinth[D]. Guangzhou:Guangdong University of Technology,2012:17-24(in Chinese)
• [36]刘侯俊，李雪平，韩晓日，等．铁镉互作对水稻脂质过氧化及抗氧化酶活性的影响[J]．应用生态学报，2013,24(8):2179-2185Liu H J,Li X P,Han X R,et al. Effects of Fe-Cd interaction on the lipid peroxidation and antioxidative enzyme activities of rice[J]. Chinese Journal of Applied Ecology,2013,24(8):2179-2185(in Chinese)
• [37]张慧君，余晨晨，余静，等．镉对火龙果种子萌发及子叶抗氧化酶活性的影响[J]．分子植物育种，2016,14(11):3172-3176Zhang H J,Yu C C,Yu J,et al. Effects of cadmium on seed germination and antioxidant enzyme activity of cotyledon in pitaya[J]. Molecular Plant Breeding,2016,14(11):3172-3176(in Chinese)
• [38]兰心宇，王军军，阎蕴运，等．水生植物有翅星蕨(Microsorum pteropus)对镉的超富集能力及抗性生理研究[J]．中国科学:生命科学，2017,47:1113-1123Lan X Y,Wang J J,Yan Y Y,et al. Hyperaccumulation capacity and resistance physiology of Microsorum pteropus,an aquatic fern to cadmium[J]. Science in China Series CLife Sciences,2017,47:1113-1123(in Chinese)
• [39] Dong J,Wu F,Zhang G. Influence of cadmium on antioxidant capacity and four microelement concentrations in tomato seedlings(Lycopersicones culentum)[J].Chemosphere,2006,64(10):1659-1666
• [40]刘玥，郭文强．中华结缕草和皱叶酸模对重金属铅和镉胁迫的生理生化响应[J]．土壤通报，2016,47(3):730-737Liu Y,Guo W Q. Response to the stress of heavy metals Pb,Cd on the physiological and biochemical characteristics of Zoysiasinica hance and Rum excrispus[J]. Chinese Journal of Soil Science,2016,47(3):730-737(in Chinese)
• [41]郭世财，杨文权．重金属污染土壤的植物修复技术研究进展[J]．西北林学院学报，2015,30(6):81-87Guo S C,Yang W Q. Research progress of phytoremediation technology to remediate heavy metal contaminated soil[J]. Journal of Northwest Forestry University,2015,30(6):81-87(in Chinese)
• [42] Vázquez S,Agha R,Granado A,et al. Use of white lupin plant for phytostabilization of Cd and As polluted acid soil[J]. Water,Air,and Soil Pollution,2006,177(1-4):349-365
• [43]谷金锋．大兴安岭典型采矿迹地土壤重金属污染分析与生态恢复研究[D]．哈尔滨:东北林业大学，2014:52-53Gu J F. Research on soil heavy metal pollution and ecological restoration in representative abandoned mining land of Da Xing’anling[D]. Harbin:Northeast Forestry University,2014:52-53(in Chinese)
• [44] Rahman M,Khatun S,Ali S,et al. Morpho-physiological diversity of root nodule rhizobia from mimosa(Mimosa pudica L.)and water mimosa(Neptunia oleracea L.)[J].Journal Bacteriology and Mycology,2018,5(1):1061
• [45] Suppadit J. Efficacy of water mimosa(Neptunia oleracea Lour.)in the treatment of wastewater from distillery slops[J]. Philippine Agriculturalentist,2008,91(1):61-68
• [46] Singha L S,Bawari M,Choudhury M D. An overview on Neptunia oleracea Lour.[J]. Assam University Journal of Science&Technology,2010,6(1):155-158