饶海鸥;查金苗;王子健;王卫民;

• 1:华中农业大学水产学院
• 2:中国科学院生态环境研究中心环境水质学国家重点实验室

摘要(Abstract)：

细胞凋亡是调控机体发育和维护内环境稳定的重要机制。在克隆了稀有鮈鲫(Gobiocypris rarus)肝脏组织中凋亡相关的bcl-2、apaf-1、caspase-3和caspase-9基因的部分cDNA片段之后,进行了序列分析。结果表明,caspase-3和caspase-9基因片段与唐鱼(Tanichthys albonubes)相对应的核苷酸序列同源性最高,而bcl-2、apaf-1基因片段则分别与鮈鱼(Gobio gobio)和鲤鱼(Cyprinus carpio)相对应的核苷酸序列同源性最高,为99%和89%。基于稀有鮈鲫和已知物种的caspase-3基因核苷酸序列构建了系统发育树,发现稀有鮈鲫与唐鱼、斑马鱼(Danio rerio)的亲缘关系最近,而与金头鲷(Sparus aurata)、红鳍东方鲀(Takifugu rubripes)的亲缘关系较远。本研究为深入理解外源性化学物质对鱼类的毒理学机制及其生态健康风险评价提供科学数据,同时也为稀有鮈鲫在鲤科鱼类的分类及进化地位的研究提供分子水平的重要参考。

关键词(KeyWords)： 稀有鮈鲫;基因克隆;同源性分析;系统发育树

Abstract:

Keywords:

基金项目(Foundation): 国家高新技术研究发展计划(863计划)(2010AA065105)

作者(Author): 饶海鸥;查金苗;王子健;王卫民;

Email:

DOI:

参考文献(References)：

• [1]Tsujimoto Y,Finger L R,Yunis J,et al.Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18)chromosome translocation[J].Science,1984,226(4678):1097-1099
• [2]Gross A,McDonnell J M,Korsmeyer S J,et al.Bcl-2family members and the mitochondria in apoptosis[J].Genes and Development,1999,13(15):1899-1911
• [3]Zou H,Henzel W J,Liu X,et al.Apaf-1,a human protein homologous to C.elegants CED-4,participates in cytochrome c-dependent activation of caspase-3[J].Cell,1997,90(3):405-413
• [4]Kim H,Jung Y K,Kwon Y K,et al.Assignment of ap-optotic protease activating factor-1gene(APAF1)to human chromosome band12q23by fluorescence in situ hybridization[J].Cytogenetics and Cell Genetics,1999,87(3-4):252-253
• [5]Yonshida H,Kong Y Y,Yoshida R,et al.Apaf1is re-quired for mitochondrial pathways of apoptosis and brain development[J].Cell,1998,94(6):739-750
• [6]Boyce M,Degterev A,Yuan J,et al.Caspases:An ancient cellular sword of damocles[J].Cell Death and Differentiation,2004,11(1):29-37
• [7]曹文宣,王剑伟.稀有鮈鲫:一种新的鱼类实验动物[J].实验动物科学与管理,2003,20(S1):96-99Cao W X,Wang J W.Rare minnoe:A new laboratory animal in China[J].Laboratory Animal Science and Management,2003,20(S1):96-99(in Chinese)
• [8]王剑伟.稀有鮈鲫的繁殖生物学[J].水生生物学报,1992,16(2):165-174Wang J W.Reproductive biology of Gobiocypris rarus[J].Acta Hydrobiologica Sinica,1992,16(2):165-174(in Chinese)
• [9]周永欣,成水平,胡炜,等.稀有鮈鲫——一种新的鱼类毒性试验材料[J].动物学研究,1995,16(1):59-63Zhou Y X,Cheng S P,Hu W,et al.A new toxicity test organism—Gobiocypris rarus[J].Zoological Research,1995,16(1):59-63(in Chinese)
• [10]Zha J M,Sun L W,Spear P A,et al.Comparison of ethinylestradiol and nonylphenol effects on reproduction of Chinese rare minnows(Gobiocypris rarus)[J].Ec-otocicology and Environmental Safety,2008,71(2):390-399
• [11]叶妙荣,傅天佑.鱼丹亚科鱼类——新属新种记述[J].动物分类学报,1983,8(4):434-437Ye M R,Fu T Y.Description of a new genus and species of danioninae from China[J].Acta Zootaxonomica Sin-ica,1983,8(4):434-437(in Chinese)
• [12]何舜平,汪亚平,陈宜瑜,等.五种鲤科鱼类的RAPD分析兼论稀有鮈鲫的系统位置[J].水生生物学报,1997,21(3):262-267He S P,Wang Y P,Chen Y Y.The RAPD analysis for five cyprinid fishes with notes to the systematic position of Gobiocypris rarus[J].Acta Hydrobiologica Sinica,1997,21(3):262-267(in Chinese)
• [13]Vidal M C,Hoole D,Williams G T.Characterisation of cDNAs of key genes involved in apoptosis in common carp(Cyprinus carpio L.)[J].Fish and Shellfish Im-munology,2008,25(5):494-507
• [14]Jin Y X,Zheng S S,Fu Z W.Embryonic exposure to cypermethrin induces apoptosis and immunotoxicity in zebrafish(Danio rerio)[J].Fish and Shellfish Immu-nology,2011,30(4-5):1049-1054